Visium Tissue Preparation Protocol | Flash Freezing Tissue

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  • Опубликовано: 9 сен 2024
  • This series of How-To videos walks you through best practices for preparing tissue that will be used in a Visium Spatial Gene Expression experiment. This first video walks you through the process of flash freezing your fresh tissue, including the supplies you will need and tips for tissue handling. Next step: Embedding Frozen Tissue in OCT.
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Комментарии • 2

  • @shaimaahassan5246
    @shaimaahassan5246 2 года назад +1

    If my tissue is very small embryonic transparent that cannot transfer it by forceps its can be in eppendorf as pellet what should I do:-
    1) put isopentane in container in liquid nitrogen and put eppendof that have the tissue in the isopentane
    2) mix the embryos tissue with isopentane in the eppendorf and then emmerse it in the liquid nitrogen, in that case how to get red of the isopentane if it is necessary