Regarding using the antibiotics for primary cells : Does is it necessary to add antibiotics whenever we change the media( let us say every 24 hours) for the first passage P0 , or it is recommended to add antibiotics just for the first 24 hours ?
I was told that if you didnt do a second spin down/resuspension that there would be remaining DMSO left over and cause cell damage. Is this true? or is that extra spin causing unneccessary stress
After aspirating the wash media, gently resuspend the remaining cell pellet in a small volume of pre-warmed culture media by pipetting up and down. Suspension volume and flask size is determined by the number of cells that were recovered.
Regarding using the antibiotics for primary cells : Does is it necessary to add antibiotics whenever we change the media( let us say every 24 hours) for the first passage P0 , or it is recommended to add antibiotics just for the first 24 hours ?
I was told that if you didnt do a second spin down/resuspension that there would be remaining DMSO left over and cause cell damage. Is this true? or is that extra spin causing unneccessary stress
I could not understand what exactly happens after aspirating cells. Please explain.
After aspirating the wash media, gently resuspend the remaining cell pellet in a small volume of pre-warmed culture media by pipetting up and down. Suspension volume and flask size is determined by the number of cells that were recovered.