Regarding using the antibiotics for primary cells : Does is it necessary to add antibiotics whenever we change the media( let us say every 24 hours) for the first passage P0 , or it is recommended to add antibiotics just for the first 24 hours ?
Please reach out to our customer support team and we'd be happy to help! ms.spr.ly/6053W0NzX (please ensure the correct country is selected in the top right of your screen)
After aspirating the wash media, gently resuspend the remaining cell pellet in a small volume of pre-warmed culture media by pipetting up and down. Suspension volume and flask size is determined by the number of cells that were recovered.
I was told that if you didnt do a second spin down/resuspension that there would be remaining DMSO left over and cause cell damage. Is this true? or is that extra spin causing unneccessary stress
![Noob To Max With DRAGON REWORK In Blox Fruits [FULL MOVIE]](http://i.ytimg.com/vi/LnBMOinoOvA/mqdefault.jpg)
Regarding using the antibiotics for primary cells : Does is it necessary to add antibiotics whenever we change the media( let us say every 24 hours) for the first passage P0 , or it is recommended to add antibiotics just for the first 24 hours ?
I love your product and I am looking for it all the time in our market but most of the time it is unavailble in my country
Please reach out to our customer support team and we'd be happy to help! ms.spr.ly/6053W0NzX (please ensure the correct country is selected in the top right of your screen)
I could not understand what exactly happens after aspirating cells. Please explain.
After aspirating the wash media, gently resuspend the remaining cell pellet in a small volume of pre-warmed culture media by pipetting up and down. Suspension volume and flask size is determined by the number of cells that were recovered.
I was told that if you didnt do a second spin down/resuspension that there would be remaining DMSO left over and cause cell damage. Is this true? or is that extra spin causing unneccessary stress