DPPH Radical Scavenging Method-Total Antioxidant Capacity Assessment
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- Опубликовано: 13 янв 2019
- Principle: DPPH(1,1-Diphenyl-2-picrylhydrazyl) is a stable free radial with red color(absorbed at 517nm). If free radials have been scavenged, DPPH will generated it's color to yellow. This assay uses this character to show free radical scavenging activity.
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Your hand pouring technique is incredible bravo.
Wonderful job, systematically created video.
The Good results affirmed your great work .
thank you so much for your work, it is really a great help to me.
very good and detailed described video.. if you have other assays, please share.. will be so useful. Thank you for a such a nice video
Thank you for the informative video. I hope you don't mind me using this to demo to my students. Thumbs up!
Thank you so much!!! This was very useful for my IBDP extended essay research !!!
Sameeeeeeeeeeeee
Thank you. It's very useful🙏🏻🙏🏻❤️❤️❤️
Fabulous video... Plz share FRAP and MCA also in next videos
Great work 💪
So great, but it will better if u also explain all detail. How to make standard curve, how many solution and solution to dissolve to make xx concentration in standard curve. I really need the detail
Can you brief me how to carry out DPPH studies for topical cream
Thank You very much for the video, I don't see you using ascorbic acid and you listed it in your material to use
Thanks a lot for sharing the video. It's verw wll prepared.
What about ascorbic acid..when is it used? And what is the control sample that has been used during spectrophotometric assay??
What is the use of ascorbic acid? It is mentioned in the materials list but didn't appear anywhere else in the video.
Could you mention the refference to the protocol, please?
Can you plz give us the reference of your protocol....it would be of great help
wonderful thank you
haii can I know if we use a sample from a liquid ( fruit juice) that same method or different method ??
Thank you so much, can someone give me the reference please?
great video.looking for ur more videos also i need a video for well diffusion method for antibacterial actvity.
How could we determine how many mL of solvents to put into the sample?
can someone explain for me where concentration in the result table from? thank youu
?plz the color yellow is obligatory te confirm the effect or not
Hello, how did you get 1ml, 2ml, 3ml, 4ml and 5 ml for the solution series?
Can anybody explain why his calculated IC50 value is 25 mg/ml while in the table it shows 65% Inhibition at 20 mg/ml?
Hello, good video, a question, where did you get the reference for the laboratory guide? HELP
are samples soluble in DPPH solution?
What is powdered food sample? What is it in?
the referece of this principle please.
What did you use as your standard?
In earlier video, you write ascorbic acid in materials, but i cant see where or when you use in tutorial.
what is the blank composition
Will you share the research article??
I want cite this vedio, can u give link of this article where it from taken
what is purpose of ascorbic acid please? because you mentioned to ascorbic acid in materials
.
You mentioned acid ascorbic but there isn't in video please can you tell me how you used it? and please here your control was it only dpph +methanol ??
I need the protocol
What is the blank in the spectrometer?
Hi. When is it used ascorbic acid?
great video keep it up
can I know why it needs ascorbic acid for this experiment?
what you take blank solution?
what did you make in sample, and negative control
Which book you can get procedure ..name pls ...
Any other methods?
Great video!
May I ask if you combined the extracts of the 2 samples?
Amazing 👌
Please mention the reference
Any refrence
You don't need a standard ?
Usually for antioxidant we take ascorbic acid standard... I'm new to this and kind of confused. Help me
Sir Please provide me flowchart of dpph assay, please🙏🙏🙏🙏
Good job
DPPH 4 mg is real at that weight??
Reference please?
May i know which food sample you used in your study
where's the paper?
Any other solvent than 99% methanol that can use???
Concentration ka column samja dijiye plz
Reference??
where is ascorbic acid function n method ?
Sir please send dpph data sheet
Where is the standard solution?
Can you please mention the reference for the protocol.
I hv the same question . Did u get the reference ?
Can you please share the %Inhibition for iceberg lettuce using the same dpph assay?
😂😂😂
Reference?? Please.
Which lab is this (location/name)? I really liked your antique centrifuge and test tube stand.
Wayamba university of Sri Lanka 🇱🇰
In table there is a column concentration mg/ml. Whose concentration doest it refers?
I have the same question, please give me answere??
Hlo please provide any reference link
excuse me I have a question,
the sample showing at the beginning, can be in liquid form???? I would be very gratful if you would answer me
yes it can be in liquid form so that eliminates the whole process of extraction and then you just have to work from the making the series dilution process.
Thanks
Hi. Im a bit confused where does extracted solution come from, the one that you used to make dilutions? Thank you
ı think it is the filtered solution from the test tubes.
Filtered solution
@@deenmediarecords4575 the filtered solution is come from one food sample only right?
Great Video! Could I ask for the references of this protocole? I am combined also about the use of ascorbic acid?
a tip: watch movies at flixzone. Been using them for watching a lot of movies recently.
@Elijah Jadiel yup, I've been using flixzone for since december myself :)
when you were making the solution series..you took 1ml, 2ml, 3ml and 4ml of the sample extract..but how much methanol did you add to it?
he completed it to 10 ml (the sign )
how do you calculate the concentration?
Are you preparing 1M or 0.1mM DPPH...I think it is 0.1mM DPPH you are preparing...
Please tell us the scientist name whose methadology you used for doing this experiment... please tell us,its so necessary to do this experiment.please text us the reference.please
Hi did you get the references?
What's the purpose of using methanol?
organic solvent
Thanks for the methodology. However, the results in the table at 7:53 are not the same as in the final graph. If we use the data available, the R^2 of the adjustment is only 0.843. The inhibition values are also incorrects in relation to the absorbance values. Why?
Because he didn't describe his standard preparation. Calculations are based on results from standard curve which we don't know because he didn't show it
%Inhibition curve in not a linear curve as far I know
great
4 mg of DDPH in 100 ml of methanol does not refer to 1M. It is too far from being so.
prepararon una solución a 0,1mM no a 1M..... con eso si toman 4 mg
Hi, can anyone help? just want to ask for this DPPH method, is it a must for using a methanol? I mean , we cant do it directly using only the dpph then adding the sample? #stilllearning
No, it is must for using methanol or ethanol.
@@maiaothanh9480 currently I'm doing my thesis, and yes, it's a must using either methanol or ethanol
no, not necessary
@@Rizuk0 Hello, im currently on my thesis as well, i might ask you what's the difference between using methanol or ethanol (I know methanol gives better performance during the extraction, but whats the use in here in comparison with ethanol)
@@STALKER1807
Hello ahmed, as for the comparison of both, I'll have to suit it up accordingly to the thesis, when it comes to DPPH assessment for some studies both solvent affects the antioxidants, and these would be one of those consideration ; using one of solvent could affects the DPPH environments for reduction, may some solvent makes the DPPH unstable as it does when reacting with the antioxidants vice versa ; solubility of both solvent should be different, thus affecting the absorbance. Hopefully these answer could useful for you.
4:19- sample+methoh
5:22- each sample 1ml mixed with 3ml dpph
6:09- make up with methoh
What is amount of methanol added to the extracted solution
Good
1:43 1mM DPPH ?
0.1mM
What is control? Is it sample abs without dpph or dpph only?
Alip Tanpanama it’s the one with ddph and ethanol without your sample
control is DPPH abs.
Dpph a simple assay/antioxidant a big term
I wanted to ask something.....is 58% a good antioxidant value?
the question is what was the concentration of the sample you tested... later on you can check what is the parallel concentration of a known molecule (like vitamin e for example)
@@MestebanK my sample was 3ml
7:54 Calculate Inhibition% by hand i.e. ((0.301-0.284)/0.301)*100%=5.65% not 5.86%. All values of Inhibition% are incorrect.
Thanks
Reference??
Hi, please did you get the reference??, I'm manasseh from Ghana
@@otabila3830 check out
Blois (1958) and Yamasaki et al. (1994)