You are my teacher of the year! I am writting a scientific work about g. mapping, i listen to your lectures and read some literature and surt the internet and I CANT STOP. this is so so intresting and exciting!!
Your best teacher man I am students of zoology from Amravati university I am watching your videos since 1 year but this best one thanks .can upload something about host spot of mutation
isn't it possible that random elimination will effect in complementation ....and makes failure of hybridoma cells(which then no longer be) survival? ....Also whts the real need of hybridoma in this process of mapping?
Hybridoma cell lines are more sustainable than human cell lines and uphold the expression of monoclonal ABs. Go through the reference: 10.1186/s43141-021-00264-6
Why we form hybridoma.. we need to know about relationship between 2 gene by gene mapping..Why we can't run the human chromosome on electrophoresis directly to know about gene mapping..What is the requirement of formation of hybridoma here?
still not getting why would we need a hybridoma in the first place...can't you just take a human cell, take out the genome (which has all the human chromosomes), amplify the chromosomes/genes, run gel, and do all the relative placement thing? and if in the hybridoma case you use 1000s of cell lines, can't you just take thousands of human cells and run the gel? what's the point of making hybridomas anyway? thanks
@Raissa Regina Ng that mean before electrophoresis we must know a marker specific to a chromosome otherwise only microscopical examination was enough? Am I right?
Sir, somatic cell hybridization and somatic cell genetics-an alternative approach to gene mapping are thes both same? Please let me know at the earliest..it will be a great help for me.. Thanks and regards
What kind of two cells can be fused together? Can we fuse every type of different cells together or is there any t&c for two cells to be fused?? Thank you for great effort in that age 🙂 Always a great inspiration for me Thank you.
@@shomusbiologyofficial Sir Your are so talented❤️ ( sir mera b sapna hai zoology me master Banu par mera knowledge itna acha nahi hai😞😞) sir ab mera +3 3rd year( 5th sem.) chal raha hai. Sir mujhe ashirbad de taki Mera bhi MSc. Entrance Clear ho aur ek acha University me addmission kar paun.
3:23 "Having a fused nucleus..." 2:49 "Nucleus never fused with each other" What is happening here ? Do you have any idea of what you say ? Had to dislike the video
may be in case of human and mouse this fusion of nucleus is not possible but once we dissolve the walls with PEG we have three alternatives.... 1.their nucleus fuse along with cytoplasm(Synkaryon) 2.only cytoplasms fuse but not the nucleus(both the nucleus remain in the cytoplasm)(heterokaryon) 3.the cytoplasms fuse but during fusion one of the nucleus dies.(Cybrid) May be sir had to specify"never fuse" only because of the case of mouse and humans.
You are my teacher of the year! I am writting a scientific work about g. mapping, i listen to your lectures and read some literature and surt the internet and I CANT STOP. this is so so intresting and exciting!!
All the very best. carry on.
Suman Bhattacharjee thank you! good luck to you too
Sir what is cell line plz explain it
this teaching methodology can only be delivered by a legend
Thank you so much for appreciating my efforts
@kasaf rasul soo true
This is the best explanation till date. Thank you Sir.
You're welcome. Glad to hear that you're getting benefit from my lectures
Your best teacher man I am students of zoology from Amravati university I am watching your videos since 1 year but this best one thanks .can upload something about host spot of mutation
I had Never imagined it would be so easy to understand n interesting too
Thank you. Glad you liked my lectures
Easy to understand. U are a gift of god
Thank you so much for appreciating my efforts
Thank you so much 😊.. U helped me a lot in my competitive exam...
You're welcome
Happy teacher's day sir...
i was wondering for this concept but you cleared my all douts 🙂you are awesome sir
Thank you so much for appreciating my efforts
Sir , this video is really very nice and conceptual. I understand each and every little concept or reason behind each and every step. 👍👍👍
really informative.,.and easy to understand .,thank u
You're welcome. Glad to hear that you're getting benefit from my lectures
yeah recently i subscribe your channel.,.one thing is very bad for me that i have lost much of my time .,.and now in exam i found u r channel
thank yo so much. you are the best!
You're welcome
isn't it possible that random elimination will effect in complementation ....and makes failure of hybridoma cells(which then no longer be) survival? ....Also whts the real need of hybridoma in this process of mapping?
Sir this video is really helpful. Could you please tell me which book to follow and which chapter should I read for this topic?
Sir if we want to obtain Human cell then Why we need to fuse Human cell with mouse. We can talk direct human cell
I have the same question right now... Why do we need to have Hybridomas we can consider human cell chromosomes only.
To make random elemention of human chromosomes to make different cell lines 🤔🤔
I have the same question too.
We need rodent (mouse ) cell for the cloning process due to this we need to fuse them
Hybridoma cell lines are more sustainable than human cell lines and uphold the expression of monoclonal ABs. Go through the reference: 10.1186/s43141-021-00264-6
Sir if we cultivate the cell having only human chromosomes than what is importance of fusion..We can simple replicate it via normal subculturing .???
So what is the reason for making hybridoma? If we use only human DNA for mapping
Best explanation ❤️❤️
Thank you so much for appreciating my efforts
Thank u for being life saver
Thank you so much for appreciating my efforts
Thank you! This really helped for my genetics exam :)
Why we form hybridoma.. we need to know about relationship between 2 gene by gene mapping..Why we can't run the human chromosome on electrophoresis directly to know about gene mapping..What is the requirement of formation of hybridoma here?
Sir Why somatic cell hybridization is required
Very clear and helpful, thank you!!
it's really helping , thank you so much
If finally we want human genome only.... Then why we are fusing it with mouse genome???
Please explain
Thnku so much for clearing this topic
how to they identify the genes at electrophoresis to they put a tag ?
still not getting why would we need a hybridoma in the first place...can't you just take a human cell, take out the genome (which has all the human chromosomes), amplify the chromosomes/genes, run gel, and do all the relative placement thing? and if in the hybridoma case you use 1000s of cell lines, can't you just take thousands of human cells and run the gel?
what's the point of making hybridomas anyway?
thanks
To make miniature human rat yeti's. Duhh!
@Raissa Regina Ng that mean before electrophoresis we must know a marker specific to a chromosome otherwise only microscopical examination was enough? Am I right?
To decrease the chromosome number we need hybridoma
also with this work also in diffrentiating between genes of the same tissue type
Nice video i understood it
You're welcome
Thankyou sooo much sir....
You're welcome
Sir, somatic cell hybridization and somatic cell genetics-an alternative approach to gene mapping are thes both same? Please let me know at the earliest..it will be a great help for me..
Thanks and regards
Will u pls tell me are these both same ?
What is cell line?
thank you sir so much
You're welcome
Awesome video sir... it helped me a lot.... :)
It is interesting! Thank you.
What about synkaryon??
What kind of two cells can be fused together?
Can we fuse every type of different cells together or is there any t&c for two cells to be fused??
Thank you for great effort in that age 🙂
Always a great inspiration for me
Thank you.
Sir topics Hindi me nhi h kya Apke pas
it's really interesting!!! Thanks a lot!
sar plass hindi use in lacture
Please ka spelling thik karo. Not all individuals in India understand hindi
@@shomusbiologyofficial ok sar i agree
very intresting
❤️❤️❤️
Thank you
Thank you
Lets find out how to create the next generation of mutants from somatic hybridisation human-mouse XD PLANET of 'HUM-RATS'
Sir Your age before 8years ago🙂
I was in my master's
@@shomusbiologyofficial Sir Your are so talented❤️ ( sir mera b sapna hai zoology me master Banu par mera knowledge itna acha nahi hai😞😞) sir ab mera +3 3rd year( 5th sem.) chal raha hai. Sir mujhe ashirbad de taki Mera bhi MSc. Entrance Clear ho aur ek acha University me addmission kar paun.
Cant understand
Boht complicated hy😥
Ye topic
3:23 "Having a fused nucleus..."
2:49 "Nucleus never fused with each other"
What is happening here ? Do you have any idea of what you say ?
Had to dislike the video
may be in case of human and mouse this fusion of nucleus is not possible but once we dissolve the walls with PEG
we have three alternatives....
1.their nucleus fuse along with cytoplasm(Synkaryon)
2.only cytoplasms fuse but not the nucleus(both the nucleus remain in the cytoplasm)(heterokaryon)
3.the cytoplasms fuse but during fusion one of the nucleus dies.(Cybrid)
May be sir had to specify"never fuse" only because of the case of mouse and humans.