TNP #22 - Zeiss Axioskop 2 MOT LED Retrofit Revisited & Bright/Dark Field, Polarization Microscopy
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- Опубликовано: 7 окт 2022
- In this episode Shahriar returns to the microscope LED upgrade challenge. The highest light density LED is used as a point source to improve Kohler illumination. Both transmission & reflected light sources are upgraded while maintaining the control purely through the microscope controls. The LED used can be found here:
www.digikey.com/en/products/d...
Several images are also presented using bright field, dark field as well as polarization manipulation.
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3:45 Thorlabs MCWHL5-C4
Great video. Thanks!
What an awesome coincidence that this came across my suggested videos-- I'm looking to retrofit a zeiss with an LED source as well! This is such a useful walkthrough of the design considerations you had to think through, thanks for uploading.
Im in exactly the same position and this is nothing like my other YT views. Wonderful and maybe a little creepy.
Same here!
I have a feeling if you tried to get 6000K with a halogen lamp, you would end up with a very short lived plasma arc lamp.
But imagine the glow!
Non Stocked. Two words that strike fear into geeks everywhere.
Nice project by the way :)
I've put the same led in flashlight last year. Its a monster with proper reflector
I love this! I have a new respect for microscopes. Thanks for sharing.
6000K with tungsten filament? ;-D
Great microscope btw, had one at work once, along with Hitachi S4800 SEM, awesome toys!
Yes, they are! Seeing how much life there is in a drop of water I collected from the local pond was amazing.
Congratulations on 100k subscribers. I always love you video. I learned a lot..
This video is the bee's knees! 😉
Oh come on
Ha!
I laughed till I cried - Where's my napkin?
Very nice microscope setup, congrats!
The 3D printed cooling frame is a nice addition and it is welcomed in order to cool the led properly, nice job. 👍
This video like all your videos are always awesome. Much appreciate you sharing your knowledge.
Awesome video, more please !...cheers.
Impressive results
I was waiting for the phase contrast images as well.... Nice microscope you have (yoda says).
If you're looking for suggestions on parts to look at with the new setup, here's a few I've wanted to see teardowns of:
* BUF802 (3.1 GHz JFET amplifier)
* ADL5569 (6.5 GHz dual differential amplifier)
* ADL5205 (1.7 GHz dual differential amplifier, 14-26 dB digitally adjustable gain plus 0-23 dB digitally controlled step attenuator)
+1 on the BUF802
About 10 yrs. ago , I had a chance to buy a Zeiss electron microscope for about $1500 from the U of M , but I had no place to put it .
Told some friends about it that I lamented not being able put some place - they said " what would I do with it " , this is inquiring minds differ - I said what else , I would look at stuff - great fun ;)
Thanks agian
The new laser headlamps have high intensity small point sources of light. A few tear-downs on RUclips as well.
Nice solution and mod.
Quite Nifty !
Thanks for insights!
The fan moulding was a nice design.
Love it
Great you returned to this project. Regarding the LED driver I guess with a few discrete components you could have saved some space and time developing it. Nonetheless this way you can address the younger folks 😉. And yes you are absolutely right in promoting microscopy and telescopes. If you got phase ring objectives it might be worth checking out Nomarsky phase contrast on this instrument.
Also I hope you remember my suggestion about extended depth of focus with a single acquisition using cubic phase plate inside the DIC slider. I think this could help in your efforts viewing multiple layers of IC's. Anyway Keep up the good work.
Lutz
Thank you Lutz. All the objectives on the microscope are HD DIC except for the 2.5x which is only HD. They are all Epiplan-Neufuar and a few have the "EC" marking. I have the DIC prisms for both reflected/transmitted light also installed. Some of the NA of the lenses and DIC prisms don't quiet match. In your view does that make a big difference? I'd also love to learn more about the cubic phase plate and how to find/install one.
@@Thesignalpath Shariar, Fluar (glass contains fluor) objectives are the best and consequently most expensive ones. Epiplan's are preferably used for material science less so for Brightfield (transmitted). Zeiss has a huge catalog for the objectives. Phase ring objectives are marked PH 0...3 to be matched with the condensor revolver. I was thinking that you might got some since you have a dark field stop. If there are more stops available they are also labeled with PHx. In your case you likely got DIC stops for the Wollaston prism. DIC is less sensitive to a mismatched NA than a phase ring would be with Nomarsky contrast. Nonetheless the prism in the slider should fill the back aperture. Otherwise when you apodize the aperture with a misfit slider you lose effective NA and with that resolution. Looks like you got a good mix of accessories. Also note that most specialty contrast methods can be achieved computationally (well that is the main business of my company). To get cubic phase plates should not be a big problem. You can 3D print a DIC slider to put it in. Well I think you know how that works. I had sent you some Matlab code that simulates forward problem and inverse solution. Just email me if you need more info on this.
On second thought reading your reply again: A Neofluar is corrected for a wide range of wavelengths up to near UV. It's also good for Brightfield and very often used in wide field fluorescence. I'll try to find the catalogs and let you know.
I wonder if one of those laser headlight modules could be retrofitted. They use a laser pointed at a phosphor to create a high intensity point source.
Yeah but that would be too much intensity and instrumental overkill. Professional illuminators (like Colibri) all just use LED's
At some point you will start to damage the sample and of course your eyes. Especially for transmitted light microscopy, if you are not careful.
Ha, now we know what happen to the ant in previous video! :-D
HAL 100? Is that one of the early HAL 9000 prototypes?
Please consider adding a thermal cutout, e.g. 'Klixon' on the heatsink, in series with the LED, just in case the fan fails. Also endearing to see you saying "degrees Kelvin" :p when the Kelvin-scale is absolute.
Thanks! Yes, Kelvin doesn't need "degree " modifier. Actually, none of the measurement scales technically do.
I saw that the LED only had CRI70 which coloring index is pretty poor, I would have used one with much higher and absolutely minimum CRI85, preferably CRI90 or even higher. Much more expensive but worth it.
You can't fool us: that's not a napkin, that's a hairball from Pooch :-)
very cool images! I believe Zeiss choice of halogen bulbs has something to do with their emission spectra being as close to the natural light as opposed to the LEDs limited emissions at certain wavelengths. Top of the line microscope! Must have cost a fortune 🙂
I don't think so. This lamp house design is more than 30years old. A time before one couldn't even fathom high intensity LED's. Xenon and mercury arc lamps were the second more expensive choice. They also had smaller emission areas and hence better suited.
He salvaged it from the dumpster a year ago.
@@thomasw6169 No Way!
Many thanks for this video. I've also got an old Axioscop. Can you say which type of digital camera module you use? I've tried a few and they've been disappointing. So often end up just using the phone camera in the eyepiece. With steady positioning it's better than expected.
I use a DSLR with the adapter for it. They always give the best results.
@@Thesignalpath thanks very much!
Hey Shahriyar ,Could you do a Video about Wide band VCOs , and I would appreciate some content on frequency multipliers (circuits) ,BTW Fellow Iranian & Love your work Thanks.
If the light source should be very small, why not use a laser to excite the phosphorus of the led?
I hope the deceased lifeforms used as subjects for microscopy died naturally and were not murdered.
Pooch had already murdered it!
Lets install windows/linux to light dimmer. I hate such solutions in modern world
First :)
Joking, love your videos and learned a lot from them.