We've washed flow cells no more than five times. Some of our collaborators, however, can do it more than five times. It all depends on how much yield you need and how many active pores remain after each run.
Hello.. I am interested in this product for research purposes but I have a question… can this product be used without the dna library? What if I want to analyze a sample from an unknown organism? What do you recommend?
Currently, the product requires the DNA library preparation step. You can extract DNA and perform Nanopore sequencing. The organism name is then determined by analyzing the DNA sequences with WIMP (EPI2ME).
We remove the waste before and after adding the solution. Sometimes we reused a flow cell by adding A and B. If we do not remove the waste before washing again, then, we cannot add solution S. Because it's full of liquid in the waste area.
I could not find any procedure for drawing the waste before adding solution A. On current version (WKE_1012_v1_revN_08Apr2016) of protocol mentioned that remove all buffer from the waste section after you added 500 ul of Storage Buffer. So, to reuse flowcell (keep at 4C), we probably have to follow; 1) Open Priming port, Add150 ul of Solution A. 2) wait for 10 min. 3) Add 500 ul of Storage Buffer. 4) Close Priming port, remove all buffer from the waste port. 5) stored at 4-8C".
@@Matsu9Matsu I'm also confused. The protocol didn't say anything about removing any solution before adding buffers. But it seems we do need to remove waste solutions before adding buffers to avoid any overflow.
Your friend can count how many active pores there are. If the activate pores > 600, he/she may wash and reuse the flowcell. I would, however, use the washed flowcell for testing purposes rather than production.
You can download the program from nanopore community web site. They have instruction "how to install and use the program in detail. community.nanoporetech.com/protocols/experiment-companion-minknow/v/mke_1013_v1_revam_11apr2016
@iam Nobody depends on the sample you use. For high purity DNA, we can reuse 2-3 times. Using high modified DNA (e.g.methylation), maybe just one time.
please I need information
how to get the software and how to install the software
How many times can the flow cell be used when cleaned properly?
We've washed flow cells no more than five times. Some of our collaborators, however, can do it more than five times. It all depends on how much yield you need and how many active pores remain after each run.
Hello.. I am interested in this product for research purposes but I have a question… can this product be used without the dna library? What if I want to analyze a sample from an unknown organism? What do you recommend?
Currently, the product requires the DNA library preparation step. You can extract DNA and perform Nanopore sequencing. The organism name is then determined by analyzing the DNA sequences with WIMP (EPI2ME).
I remember that the protocol says drawing the waste after adding solution A and S. Maybe the protocol has been updated?
We remove the waste before and after adding the solution. Sometimes we reused a flow cell by adding A and B. If we do not remove the waste before washing again, then, we cannot add solution S. Because it's full of liquid in the waste area.
I could not find any procedure for drawing the waste before adding solution A. On current version (WKE_1012_v1_revN_08Apr2016) of protocol mentioned that remove all buffer from the waste section after you added 500 ul of Storage Buffer. So, to reuse flowcell (keep at 4C), we probably have to follow; 1) Open Priming port, Add150 ul of Solution A. 2) wait for 10 min. 3) Add 500 ul of Storage Buffer. 4) Close Priming port, remove all buffer from the waste port. 5) stored at 4-8C".
@@Matsu9Matsu I'm also confused. The protocol didn't say anything about removing any solution before adding buffers. But it seems we do need to remove waste solutions before adding buffers to avoid any overflow.
what happens if you forget to wash a flowcell and leave it out overnight? is it still reusable? (asking for a friend)
Your friend can count how many active pores there are. If the activate pores > 600, he/she may wash and reuse the flowcell. I would, however, use the washed flowcell for testing purposes rather than production.
I have problems with the software please help me
You can download the program from nanopore community web site. They have instruction "how to install and use the program in detail.
community.nanoporetech.com/protocols/experiment-companion-minknow/v/mke_1013_v1_revam_11apr2016
@iam Nobody depends on the sample you use. For high purity DNA, we can reuse 2-3 times. Using high modified DNA (e.g.methylation), maybe just one time.
@iam Nobody Yes. My lab usually buys 48 flow cells batch which allows us to get a cheaper price per flow cell.