Thank you for your detailed explanation According to your experience, if you perform a whole transcriptome experiment on mouse cells and add 12 barcodes, can a flow cell measure the differential expression data of the whole transcriptome? Thank you so much!
How many samples can be prepaired with one kit? Do you need 1 kit and 1 cell per sequencing run or is the kit enough for multiple runs?
You can run up to 96 samples with the right kit
Hi. Thank you for the detailed video. If I want to get 0.5GB data per sample, how many samples can I combine and load in one flow cell?
8:11 "gently open the Spot ON cover, to expose the loading airport" lol.
Keep up the good work👍🏾
Very well demonstrated.. keep it up..
Thank you for your detailed explanation
According to your experience, if you perform a whole transcriptome experiment on mouse cells and add 12 barcodes, can a flow cell measure the differential expression data of the whole transcriptome?
Thank you so much!
Nice video. Is this done using only the ligation kit reagents in the startup package? What kind of DNA are you sequencing (PCR product)?
its possible send the steps protocol?
What amount of FLT did you guys put in the sample?
What kind of flow cell is this?
Thank you for the video tutorial! Do you have everything written down?
All protocols are available in ONT community
@@matanoren2220 dear sir, what is ont community? From where we can find?
@@Hellowh40nanopore Oxford technology
One question, is the pcr really necessary? I was told it could be done without it.
They are probably maximizing thier return
How many Times can you use a flow cell?
Just once. But you can multiplex samples through barcoding
Potentially several times until performance is going down. You can check the available pores before each run
Pls upload new video regarding this tech if possible.
is this machine able to sequence a whole human genome?
Thanks. very useful
Very nice vdo.
i wonder myself if this is anonymous , do you need to be connected on internet to run it ? I v heard that informations comunicate on theirs servers.
It is anonymous and it can be run without internet (other then updates )
Does it offer the code in full form of all DNA? Some chromosome? Or the sequence of some gene?
Depending what you sequence. This is the rapid protocol, so it will sequence parts of the target sequence (either a genome or a PCR product)