Hello! I’m glad I found your video, although for someone like me, maybe not clear enough without enough prior knowledge. The reason why I am here is because of the current use of PCR tests in determining the spread of the newly discovered virus, now also its mutated version. We all see the numbers of infected people, mainly, if not solely, determined by the result of these tests. I have listened to interviews of Kary Mullis, the inventor of the test, who as I understand was worried about the test being used in the wrong way. However, I still am not sure how the test could be used in the wrong way, and if that is true. I have heard that the PCR test are not an accurate way to measure the spread of for instance COVID-19. What are your thoughts on this and do you believe PCR tests are a good method for measurement?
Thank you for your message! The one and only purpose of PCR is to amplify a very specific sequence of DNA. The specificity comes along with the primer. We have 4 Bases in the genome (A, C, G and T). A pairs with T and C with G. Let the sequence of the Corona virus be ACCCCTTTTAAT.... and so one. Further, we assume this code is so unique - it is not present in genomes of other viruses or organism (not in us). If we now design a primer which is complementary to that sequence: TGGGGAAAATTA and provide the correct temperature and other ingredients, this sequence and just this distinct one will be amplified. Later a band is seen on a gel which tells us ˋyes he has covid´. Another patient does not have the virus and comes for testing. The sequence is not present in bacterial genomes inside him neither in his own genome. Primers can not specifically bind and nothing will be amplified. Hence no signal in a gel -> negative ˋno covid´. Now, for simplicity in this explanation, I assumed genomic material of SARS CoV2 is DNA, but in fact it is RNA which is first reverse transcripted into DNA also with unique selection of primers. Kary Mullis did not intend to invent PCR for the detection of a virus - yes but that doesn’t mean it can’t work. Viagra was not intended to treat erectile dysfunction in the first place, but pulmonary arterial hypertension - but both is possible. PCR just needs nucleic acid like DNA or RNA to amplify that. Viruses contain nucleic acids. If the primers are unique for this sequence, PCR can definitely detect a viral infection, yes! Hope that was helpful, my friend! Right now, I am working on a new video on the detection of Covid19. You may also watch my antigen rapid test video. Cheers
when i dont understand something in biotechnology i always check your channel first. Youre doing great work!!!
Thanks Henrik, your work is great, it is a shame that it is so underrated
Nice presentation. I wasn't aware of the magnesium issue. Great information.
Thank you, I really needed this for my project on PCR 👍
Crystal clear explanation 👏
Hello!
I’m glad I found your video, although for someone like me, maybe not clear enough without enough prior knowledge. The reason why I am here is because of the current use of PCR tests in determining the spread of the newly discovered virus, now also its mutated version. We all see the numbers of infected people, mainly, if not solely, determined by the result of these tests. I have listened to interviews of Kary Mullis, the inventor of the test, who as I understand was worried about the test being used in the wrong way. However, I still am not sure how the test could be used in the wrong way, and if that is true. I have heard that the PCR test are not an accurate way to measure the spread of for instance COVID-19. What are your thoughts on this and do you believe PCR tests are a good method for measurement?
We need an answer to this
Thank you for your message! The one and only purpose of PCR is to amplify a very specific sequence of DNA. The specificity comes along with the primer. We have 4 Bases in the genome (A, C, G and T). A pairs with T and C with G. Let the sequence of the Corona virus be ACCCCTTTTAAT.... and so one. Further, we assume this code is so unique - it is not present in genomes of other viruses or organism (not in us). If we now design a primer which is complementary to that sequence: TGGGGAAAATTA and provide the correct temperature and other ingredients, this sequence and just this distinct one will be amplified. Later a band is seen on a gel which tells us ˋyes he has covid´. Another patient does not have the virus and comes for testing. The sequence is not present in bacterial genomes inside him neither in his own genome. Primers can not specifically bind and nothing will be amplified. Hence no signal in a gel -> negative ˋno covid´. Now, for simplicity in this explanation, I assumed genomic material of SARS CoV2 is DNA, but in fact it is RNA which is first reverse transcripted into DNA also with unique selection of primers. Kary Mullis did not intend to invent PCR for the detection of a virus - yes but that doesn’t mean it can’t work. Viagra was not intended to treat erectile dysfunction in the first place, but pulmonary arterial hypertension - but both is possible. PCR just needs nucleic acid like DNA or RNA to amplify that. Viruses contain nucleic acids. If the primers are unique for this sequence, PCR can definitely detect a viral infection, yes!
Hope that was helpful, my friend! Right now, I am working on a new video on the detection of Covid19.
You may also watch my antigen rapid test video. Cheers
@@undergroundking498 this might help a little
ruclips.net/video/dVVm1FAiRgI/видео.html
@@henrikslab but why are those test reacting with cola, some juices and others and shows that covid is present. How is that possible?
i vill nau post a queschion in ze commens
Nice and informative video
Best video on PCR...
Inventor of PCR Testing Kerry Mullis Full Presentation on his Stance Against the Science of Aids.
So, is this a good technique for accurate CV 19 testing?
No, the inventor said the pcr test doesn’t test for viruses nor illness.
No, It's not even a test.
John Sergei
The pcr investor in his own words!
ruclips.net/video/iWOJKuSKw5c/видео.html
This is literally making DNA. It’s not a test.
Pssst. Its a DNA test.
thank you so much it helped me so much
Simple and easy 👌