Could this centrifuge technique be used with pond water to concentrate the lifeforms at the bottom and removing excess water that are near void of lifeforms?
This depends on the density of the particle (has to be denser than water) and also if the particle forms bonds with the water. Eg. Sodium salt (Na+) is denser than water but can not be centrifuged because it interacts with water (the negatively charged oxygen of the water interacts with the positive Na+). In other words, the substances can not be dissolved. Bacteria (1 micrometer) can be centrifuged but you need fast spinning centrifuges for that.
You have done one with honey, would like to see one with fermented honey, they say that starts to loose its antimicrobial ability and through fermantation (just cut some fruit some hrs before in it) you get colonies going on...
Nice video and very helpfull that you demonstrated this. Small remark: better take honey from flowers. Waldhonig is the excretion from insects (Aphid) that is collected by bees. Theoretically there is no pollen in it. Although there is always a bit of "contamination" from nectar from flowers and that is what you observed.
I assume you need "raw honey" for this purpose. The honey you can buy in grocery stores is filtered, to remove pollen so that it is less likely to crystalize. Am I right?
Hi, Thank you for uploading all these videos. Can you tell me how to observe aerobiological samples collected on melinax tapes from spore traps? I have collected pollen and spores from air but my slides are not very clear. Any tips to clearly observe these?
Hello Microbe Hunter, can you tell me how I can see inside daffodil pollen in order to see the nucleus, I think there a two I know it can be done but do not know how to start.
I doubt that you can do it well - the adverts for this microscope say it comes with 5x,10x,15x magnification. Most people tend to work with 40x for pollen identification.
I think its because u can only focus on one thin layer at a time like a cross section of it. He focus's in and out so u can see the layers. Its alot of hassle to make a composite image to see the whole thing. This guy literally shows u a live camera feed. Its sick this channel love it
I think the mickey-mouse looking one is from a conifer. If I recall, the "ears" make the pollen buoyant when they land inside the female cone and allow them to move up through drops of water in order to fertilize the ovum. It's been a while since I've taken plant physiology though, so look into this more if you're interested.
![FendiDa Rappa - Clock Dat ft. Shamar Marco [Official Music Video]](http://i.ytimg.com/vi/3NBU8KHdxYY/mqdefault.jpg)
Could this centrifuge technique be used with pond water to concentrate the lifeforms at the bottom and removing excess water that are near void of lifeforms?
Yes, of course. As a matter of fact, this is a common procedure to do.
Thanks, the centrifugues are great. Great tip!
Your shows are very informitave
Any palynologist around to identify these pollen grains
What is the minimum particle size that can be settled by centrifugation technique?
This depends on the density of the particle (has to be denser than water) and also if the particle forms bonds with the water. Eg. Sodium salt (Na+) is denser than water but can not be centrifuged because it interacts with water (the negatively charged oxygen of the water interacts with the positive Na+). In other words, the substances can not be dissolved. Bacteria (1 micrometer) can be centrifuged but you need fast spinning centrifuges for that.
@@Microbehunter Thank you for the information :)
You have done one with honey, would like to see one with fermented honey, they say that starts to loose its antimicrobial ability and through fermantation (just cut some fruit some hrs before in it) you get colonies going on...
That's a great video!
Nice video and very helpfull that you demonstrated this. Small remark: better take honey from flowers. Waldhonig is the excretion from insects (Aphid) that is collected by bees. Theoretically there is no pollen in it. Although there is always a bit of "contamination" from nectar from flowers and that is what you observed.
i love your homemade centrifuge suggestion at the end - I was wondering what to do about not having one
You can allow the pollen to settle over night.
I assume you need "raw honey" for this purpose. The honey you can buy in grocery stores is filtered, to remove pollen so that it is less likely to crystalize. Am I right?
Pure honey never dissolves...
Dear guy, Ty you for this video ! I'm beekeeper and you helping me with this vidéo.
Hi, Thank you for uploading all these videos. Can you tell me how to observe aerobiological samples collected on melinax tapes from spore traps? I have collected pollen and spores from air but my slides are not very clear. Any tips to clearly observe these?
Hello Microbe Hunter, can you tell me how I can see inside daffodil pollen in order to see the nucleus, I think there a two I know it can be done but do not know how to start.
What’s is the source of your honey?
This was honey from a local farmer. So it was natural honey.
Dear Sir, can I analyse pollen with L-101 675X China Microscope ?
I doubt that you can do it well - the adverts for this microscope say it comes with 5x,10x,15x magnification. Most people tend to work with 40x for pollen identification.
what was the zoom of the microscope? X ?
It both shows in the bottom left hand corner of the video (20x and 60x), and he also repeats the same information in the commentary.
Your video is very helpful!!! Thank you!!
can I use 10x and100x for this..?
So nice video
Why it can't be focued well? The image is blure
I think its because u can only focus on one thin layer at a time like a cross section of it. He focus's in and out so u can see the layers. Its alot of hassle to make a composite image to see the whole thing. This guy literally shows u a live camera feed. Its sick this channel love it
I think the mickey-mouse looking one is from a conifer. If I recall, the "ears" make the pollen buoyant when they land inside the female cone and allow them to move up through drops of water in order to fertilize the ovum. It's been a while since I've taken plant physiology though, so look into this more if you're interested.