Trypsin is used because it is robust (works at a wide temp range) and most importantly cuts specifically and reliably at the C termini of Lysine and Arginine residues. That specific cutting is important because the mass spec software data base of peptides is based on this type of cutting.
I assume Trp is a more reliable enzyme since it produces positively charged amino acids Hence, the chance will be higher for these amino acids to be detected.
REFERENCE
Protein Sample Prep for Mass Spectrometry: bit.ly/3n1yrX3
Trypsin is used because it is robust (works at a wide temp range) and most importantly cuts specifically and reliably at the C termini of Lysine and Arginine residues. That specific cutting is important because the mass spec software data base of peptides is based on this type of cutting.
Thanks for schooling us, Tyler!
@@adwoabiotech Thanks for the great video!
Thanks
Happy to help.
I assume Trp is a more reliable enzyme since it produces positively charged amino acids
Hence, the chance will be higher for these amino acids to be detected.
Thanks so much for your great presentation!=)
I really enjoyed it. ^_^
Would you please also make a video for mass spect data analysis?
Thank you!
Thanks for your comment, Soha! Absolutely, I'll get on that in the new year (inshallah).
@@adwoabiotech Thanks so much dear ^-^
@@adwoabiotech Thanks so much dear ^-^
@@adwoabiotech Thanks so much dear ^-^