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Adwoa
Австралия
Добавлен 9 сен 2014
Hi, I'm Adwoa, a Master of Public Health graduate and a Biotechnologist. I enjoy learning in a relaxed and animated fashion, so I set out to share my learning style via areas and techniques I use in medical research.
Adwoa Biotech covers techniques you're likely to use in a medical research laboratory.
Are you...
📌 Analysing Western Blot Data? See ⚖️ HOW TO QUANTIFY WESTERN BLOT BANDS using ImageJ | Area Under The Peak Method
📌Performing Drug screening Assays? See 💊 DRUG DISCOVERY AND DEVELOPMENT via High Throughput Screening // UPDATED
📌 Doing CRISPR CAS9 Experiment?: See 🖥️ BIOINFORMATICS OF CRISPR CAS9 | Designing guide RNAs (gRNAs) for Genome Editing
📌Analysing Data?: See 🛑 CONCEPTS IN DATA ANALYSIS: MEAN, STANDARD DEVIATION, FOLD CHANGE & GRAPHING of data in excel
I hope you enjoy learning from these videos as much as I enjoy making them :)
Please consider subscribing if you find the content helpful.
Adwoa Biotech covers techniques you're likely to use in a medical research laboratory.
Are you...
📌 Analysing Western Blot Data? See ⚖️ HOW TO QUANTIFY WESTERN BLOT BANDS using ImageJ | Area Under The Peak Method
📌Performing Drug screening Assays? See 💊 DRUG DISCOVERY AND DEVELOPMENT via High Throughput Screening // UPDATED
📌 Doing CRISPR CAS9 Experiment?: See 🖥️ BIOINFORMATICS OF CRISPR CAS9 | Designing guide RNAs (gRNAs) for Genome Editing
📌Analysing Data?: See 🛑 CONCEPTS IN DATA ANALYSIS: MEAN, STANDARD DEVIATION, FOLD CHANGE & GRAPHING of data in excel
I hope you enjoy learning from these videos as much as I enjoy making them :)
Please consider subscribing if you find the content helpful.
FUNCTIONAL GENE MUTATION VALIDATION : A Step by Step Guide for Scientists and Students
Are you a scientist or student looking to validate the functional impact of gene mutations? This video provides a comprehensive guide on how to perform functional validation experiments. Learn about different techniques, including CRISPR-Cas9 gene editing, RNA interference, and overexpression studies. Discover the importance of proper experimental design and data analysis for accurate results. Watch now to gain valuable insights into functional gene mutation validation!
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This video is about functional gene mutation validation, gene mutation analysis, CRISPR-Cas9 gene editing, RNA interference, overexpression studies, molecular biology, genetics, experimental design, data analysis,
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This video is about functional gene mutation validation, gene mutation analysis, CRISPR-Cas9 gene editing, RNA interference, overexpression studies, molecular biology, genetics, experimental design, data analysis,
s...
Просмотров: 13
Видео
⭐ Top MALARIA TREATMENTS: Effective Strategies & Latest Advances Explained
Просмотров 8321 день назад
Discover the most effective treatments for malaria in this concise 10-minute video. We cover the latest medical advances, traditional therapies, and preventive measures to combat this life-threatening disease. Whether you're a healthcare professional, student, or just curious about malaria, this video provides essential insights into how to manage and treat malaria effectively. Learn about the ...
🛑 WESTERN BLOT MASTERY: Complete Guide to SAMPLE PREPARATION
Просмотров 132Месяц назад
Learn the art of Western blotting with this comprehensive guide to sample preparation. From sample collection to protein quantification, this video covers all essential steps for successful Western blots. Master the basics and achieve reliable results in your experiments.
10 Minutes to IMPROVE ANALYTICAL ACCURACY (Here's How)
Просмотров 132Месяц назад
Discover the importance of internal standards in analytical chemistry and how they enhance the accuracy of your experiments. This video provides a step-by-step guide on using internal standards, their benefits, and practical tips for implementation. Perfect for students, researchers, and lab technicians looking to improve their quantitative analysis skills. Subscribe for more insights into labo...
Photosynthesis: The Process That Makes Plants Better Than Us
Просмотров 1573 месяца назад
Welcome to the amazing world of photosynthesis! Ever wondered how plants turn sunlight into food? Today, we're diving into this magical process that powers all life on Earth. . . . . . . . . This video is about Photosynthesis, How Photosynthesis Works, Photosynthesis Process, Photosynthesis for Kids Photosynthesis Explained, Biology Basics Photosynthesis, Plant Food Production, Oxygen Productio...
🔬 ESSENTIAL CALCULATIONS FOR CELL CULTURE: Step-by-Step Guide
Просмотров 7193 месяца назад
Cell culture, a cornerstone of biological research, involves growing cells under controlled conditions outside their natural environment. This technique has revolutionized various scientific fields, from basic biology to applied medical research. Cell culturing has applications in the follow: 1. Fundamental Biological Research - Understanding Cell Physiology: Cell culture enables detailed study...
Power Analysis Explained: How to Calculate Sample Size for Your Research
Просмотров 783 месяца назад
Master Power Analysis: Key to Accurate Sample Size in Research
POWER ANALYSIS Explained: How To Calculate Sample Size For Your Research
Просмотров 2383 месяца назад
Power analysis is a critical step in the design of an experiment or study, used to determine the minimum sample size required to detect an effect of a given size with a certain degree of confidence. Here is a step-by-step guide on how to conduct a power analysis. . . Power analysis helps ensure your study is adequately powered to detect meaningful effects, reducing the risk of Type II errors an...
🛑 DNA Shearing and Library Prep: The Next Frontier
Просмотров 3465 месяцев назад
Imagine you need to sequence the genome of a malaria parasite.The genome size is 23 mb. Currently the machines for sequencing DNA cannot fragment that long length of DNA in one hit. They typically can handle up to 600 bp. Consequently, the input DNA must be fragmented into tiny pieces before sequencing. You have three choices for fragmenting the DNA: 1) Use enzymes that recognise specific nucle...
UNDERSTANDING BLOOD GROUPS AND TYPES: Exploring the ABO and Rh Systems🩸
Просмотров 8776 месяцев назад
You've probably heard the saying that despite all the differences we see in people, we all bleed the same blood. Well, yes and no. Like so many things in life there are nuances when you actually get down to it and that brings us to the idea of blood types or blood groups. There are a total of 33 different classification systems for blood recognised, but the two that you need to worry about most...
BIOANALYZER 2100 Protocol and Software Guide: Optimizing Your Analysis Workflow
Просмотров 2,3 тыс.7 месяцев назад
In this video we run samples on Agilent's Bioanalyzer to look at the nucleic acid distribution. First, take out the RNA dye concentrate, which is shipped to you and allow to equilibrate at room temp. for 30 minutes. We use the RNA 6000 Nano kit. You will need the gel, make a dye-mix, and use the marker and ladder shipped with the kit and agilent's Bioanalyzer instrument and software. After prep...
Converting Amount (mg) to Percent (g/100ml): Simple Calculation Guide
Просмотров 9308 месяцев назад
Solutions are sometimes described in terms of per cent concentration of solute in a solution. Percent by weight is by convention, gram of solute divided by 100g of solution. Let's look at how to perform such calculations. . . . . . . . This video is about Amount to percent conversion, Weight to volume conversion, mg to g/100ml calculation, Concentration conversion formula, Chemistry calculation...
Understanding JOURNAL RANKINGS and h-Index: Boost Your Research Impact
Просмотров 4009 месяцев назад
Discover the importance of journal rankings and the h-index in the academic world. Learn how these metrics can boost your research impact and enhance your academic career. In this video, we explain the concepts of journal ranking and h-index, provide tips on how to improve them, and discuss their significance in scholarly publishing. Whether you're a researcher, student, or academic professiona...
Demystifying Laboratory Water: Understanding the Different Types for Scientific Research 💧💦
Просмотров 9439 месяцев назад
Water quality and your experiments In case you haven’t zoomed in on it yet: there are several types of water purity needed for your experiments in the lab. The type of water you must use, depends on the technique & the level of interference the technology/technique can tolerate. Type I water is for sensitive applications (where very tiny or trace components can be detected) like Mass spectr...
🔴 Calculating Amount of Drug Needed for Cell Culture: Practical Guide
Просмотров 2 тыс.11 месяцев назад
The typical workflow for cell culture (mammalian cell culture) involves thawing cells from liquid nitrogen and culturing in appropriate nutrient media. The nutrient media often has fetal bovine serum (aka fetal calf serum), penicillin, streptomycin and L-glutamine or Glutamax. In addition to these cell culture components, some additional components may be needed. for example, If the cell has be...
🛑 CELL VIABILITY ASSAYS | MTT Assay | Alamar Blue | Propidium Iodide | Cell TIter Glo
Просмотров 3,8 тыс.Год назад
🛑 CELL VIABILITY ASSAYS | MTT Assay | Alamar Blue | Propidium Iodide | Cell TIter Glo
WHO WAS PHILLIS WHEATLEY? | A brief Biography
Просмотров 2,2 тыс.Год назад
WHO WAS PHILLIS WHEATLEY? | A brief Biography
Kizzmekia Corbett | LEAD, COVID-19 MODERNA VACCINE | Harvard T.H. Chan School of Public Health
Просмотров 414Год назад
Kizzmekia Corbett | LEAD, COVID-19 MODERNA VACCINE | Harvard T.H. Chan School of Public Health
Understanding Blood: WHOLE BLOOD vs. PLASMA vs. SERUM Explained
Просмотров 1,3 тыс.Год назад
Understanding Blood: WHOLE BLOOD vs. PLASMA vs. SERUM Explained
DROPLET DIGITAL PCR Explained: Advantages, Applications, and Protocol Walkthrough 💧 | Droplet PCR
Просмотров 3,4 тыс.Год назад
DROPLET DIGITAL PCR Explained: Advantages, Applications, and Protocol Walkthrough 💧 | Droplet PCR
Koko recipe | Hausa millet porridge | Taste of Ghana
Просмотров 529Год назад
Koko recipe | Hausa millet porridge | Taste of Ghana
Understanding Logarithms: A Beginner's Guide to Logarithms and Their Applications
Просмотров 709Год назад
Understanding Logarithms: A Beginner's Guide to Logarithms and Their Applications
📊 Graphing MEANS AND STANDARD DEVIATION in Excel | Visualize Data
Просмотров 896Год назад
📊 Graphing MEANS AND STANDARD DEVIATION in Excel | Visualize Data
👨💻 Mastering Workplace Success: Insights from Gorrick Ng's 'The Unspoken Rules'
Просмотров 253Год назад
👨💻 Mastering Workplace Success: Insights from Gorrick Ng's 'The Unspoken Rules'
🩸 Discover the DIFFERENT TYPES OF WHITE BLOOD CELLS
Просмотров 632Год назад
🩸 Discover the DIFFERENT TYPES OF WHITE BLOOD CELLS
🛑 CALCULATE LIMIT OF DETECTION (LoD) AND LIMIT OF QUANTITATION (LoQ) | Adwoa Biotech
Просмотров 17 тыс.Год назад
🛑 CALCULATE LIMIT OF DETECTION (LoD) AND LIMIT OF QUANTITATION (LoQ) | Adwoa Biotech
🧬 DNA and RNA EXTRACTION | Nucleic Acid Isolation | Adwoa Biotech
Просмотров 10 тыс.Год назад
🧬 DNA and RNA EXTRACTION | Nucleic Acid Isolation | Adwoa Biotech
🧪 MEASUREMENT OF DNA, RNA OR PROTEIN CONCENTRATION | SPECTROPHOTOMETER VS. FLUORESCENCE-BASED METHOD
Просмотров 2,1 тыс.Год назад
🧪 MEASUREMENT OF DNA, RNA OR PROTEIN CONCENTRATION | SPECTROPHOTOMETER VS. FLUORESCENCE-BASED METHOD
Yes, quality makes a lab more confident.
well explained
Thanks!
Have you been prescribed any of these antimalarials before?
Great video!! Glad our paths crossed!
Thank you!
I have to add a comment. THANK YOU! I am an attorney and I am taking a class in cybersecurity that is way more mathy than my law school-trained brain is accustomed to. This video is easy to follow and very helpful. I am glad you gave us a couple of examples to practice and also, the "why" of using the log function was interesting. I do not remember much from my trig days in high school, many years ago. Again, thank you!
Thanks for taking the time to let me know that it was helpful!
Thank you Adwoa for making this video. Most of these content are put together by the Indians and westerners. I’m glad you attempted this. I know you will serve us with even better content. You did well
I am a masters student in Biomedical Engineering and I’d need to reach out to you. Can I have your mail ?
Thanks, Godfrey, for the motivating comment.
@@kuffuor_godfred Good to know. Reach out to me on agyapomaa4@gmail.com
I should become a doctor in my next life ❤
I'm waiting for that ;) But why not medical researcher, scientist or technician? Doctors have all the publicity 😆
Thanks Adwoa! We need more teachers like you to help us understand scientific concepts better! Great tutorial
Thank you for the motivating comment!
Hi, great video. I am attempting to do this for SDS-PAGE gels rather than WB ones. I am trying to compare Rubisco content and purity from various treatments. However, unlike WB where you probe for a target protein (e.g Rubisco), and get one nice band, my SDS-PAGE gel has many bands other than my POI. In this case, where would I subtract the blank from as there are no 'empty' spaces in the lanes? I thought of taking three measurements at the top of the gel (not in the lanes) and taking an average of this as the background. Do you have any suggestions? Also, I am not sure about the case where there are multiple bands per lane and the fact that I want to find out relative purity and compare the relative quantity between treatments (using a standard curve generated from Rubisco quantitation standard) if I should use the integrated density method or the NIH (AUC) method? Please give me any tips and suggestions. Thanks!
Hi Jack, just to clarify: you have not transferred your protein unto a membrane (nitrocellulose or PVDF), you've simply stopped after gel electrophoresis (SDS-PAGE)? So did you do a commasie stain to visualise ALL the proteins? In that case, you can use any part of the gel that is not rubisco (i.e. any part that is above or below the mw of rubisco - ~56kDa), as your background. While Rubisco has a molecular mass of ∼560 kDa, it consists of eight small (∼14 kDa each) and eight large (∼56 kDa each) subunits arranged as eight heterodimers.
Here's a link to a journal article of the experiment you described, so that you can appreciate how they determined the rubisco band: www.ncbi.nlm.nih.gov/pmc/articles/PMC2689150/#:~:text=Rubisco%20has%20a%20molecular%20mass,Malkin%20and%20Niyogi%2C%202000). All the best.
@@adwoabiotech Hi, no I haven't transferred my protein onto a membrane, I have just stained it with Coomassie (visualised all the proteins). I tried using the auto background subtraction function on ImageJ (w/ 250 pixels) and this seemed to do the job, but I'm not sure if this is appropriate. Also, what is your opinion on using software such as ImageLab? I tried using that instead and think it may give more consistent results and is more intuitive. For example tried three different methods, IntDen, NIH and ImageLab. For the same protein band in the same lane, they gave differing puritys of Rubisco of 30.93% (IntDen), 41.38% (NIH) & 57.07% (ImageLab). From this, I am not sure which technique to use going forward.
Also, thanks for the link to that article, it is very helpful!
Original Paper Electrophoretic Transfer of Proteins from Polyacrylamide Gels to Nitrocellulose Sheets: Procedure and Some Applications: www.pnas.org/content/pnas/76/9/4350.full.pdf
References to deepen your knowledge : Books "Principles of Instrumental Analysis" by Douglas A. Skoog, F. James Holler, Stanley R. Crouch This book provides comprehensive coverage of analytical techniques, including the use of internal standards in various instrumental methods. ISBN: 978-0495012016 "Quantitative Chemical Analysis" by Daniel C. Harris This text covers the principles and practices of quantitative analysis, including the application of internal standards in different analytical procedures. ISBN: 978-1464135385 "Handbook of Analytical Techniques" edited by Helmut Günzler and Alex Williams This handbook includes detailed discussions on various analytical techniques and the use of internal standards to improve data quality. ISBN: 978-3527296539 Articles and Papers "Use of Internal Standards in Quantitative Analytical Chemistry" by Alan G. Marshall and Bruce S. Meyer Journal of Chemical Education, 1977, Vol. 54, No. 10, pp. 640-643. This article provides an overview of the principles and applications of internal standards in quantitative analysis. DOI: 10.1021/ed054p640 "The Role of Internal Standards in Quantitative Analysis by Mass Spectrometry" by Chris B. Stack, David Muddiman Analytical Chemistry, 2011, Vol. 83, No. 12, pp. 4649-4660. Discusses the use of internal standards in mass spectrometry to enhance quantitative accuracy. DOI: 10.1021/ac200160w "Application of Internal Standards in Chromatographic Analysis" by James W. Taylor Journal of Chromatographic Science, 2002, Vol. 40, No. 6, pp. 292-299. Explores the use of internal standards in chromatographic methods to improve quantification. DOI: 10.1093/chromsci/40.6.292 Online Resources National Institute of Standards and Technology (NIST) - Analytical Chemistry Division The NIST website provides guidelines and resources related to analytical methods, including the use of internal standards. NIST Analytical Chemistry Division International Union of Pure and Applied Chemistry (IUPAC) - Compendium of Chemical Terminology (Gold Book) The IUPAC Gold Book offers definitions and explanations of terms used in analytical chemistry, including internal standards. IUPAC Gold Book Practical Guides "Quantitative Analysis Using Internal Standards in HPLC" by Jürgen Hochstrasser A practical guide on the application of internal standards in high-performance liquid chromatography (HPLC). Available online as a technical note from various chromatography companies.
Hi! What temperature do you usually spin the sample at? Room? 4ºC? Thank you so much!
Room temp is recommended.
@@adwoabiotech Thank you!
The teaching methodology is not good...
Oh sorry to hear that it wasn’t clear for you, but thanks for the feedback. Will keep it in mind for future tutorials. In the meantime you can let me know what was not clear.
@@adwoabiotech background music... Took much of cartoons are not good...
bullshit, not helpfull at all
LOL! I appreciate the feedback. I will try to make future videos clearer. Was there any question you had in particular that I could try to answer for you?
I did DNA extraction by method of Maxi prep for the first time. I didn’t even understand the others steps. I’m willing to learn more methods and other steps.
It takes time to digest all the information but repeat it several times and it'll be locked in. The Maxiprep allows isolation of larger amounts of DNA. What was your sample source for the extraction?
@@adwoabiotech we were extracting Plasmid DNA of bacteria E.coli
Greetings from America. I love your teaching approach Adwoa. Your channel is one of my favorites! Can you create a video similar to this showing how internal standards are used for a calibration curve. From what I understand ratios are key for the internal standard approach. Any information on this would be much appreciated!
Hi and thanks for the very motivating comment! I will certainly do that. Check back in about a week.
@@adwoabiotech thank you! That would be great
@@marcoromo9252 The video that you requested is now live! Thanks for your patience and please let me know if anything is unclear.
Hello Doctor! I was diagnosed with HTLV-1, I have hyperthyroidism, osteoarthritis in my spine and osteoarthritis in my knees (I had surgery on my spine and a knee replacement). Do these bone problems and hyperthyroidism come from HTLV-1? I am 64 years old and female! A hug from Brazil!
Hello! Apologies, I'm not a doctor. I am not aware of these conditions being caused by HTLV-1, so please consult your doctor. Here's a link to a video discussing the causes of hyperthyroidism: ruclips.net/video/hxzJxCONxqk/видео.html I pray you find the underlying cause, so that you can find curative treatments. All the best!
I want to ask a question. My English skill is not enough to understand . It could be silly question sorry . I am trying to learn statistics . I would love to do statistics of my researches my own . I want to do a research about "Mortality and complications after percutaneous endoscopic gastrostomy)PEG): a retrospective one-centered study" My universe is 908 PEG . After excluding criterias I dont know how much will stay but probably around 600 cases I want them all. How can I calculate for that retrospective research sample size. 1-After PEG minör complication rate : %18-38 major complcation %2-4 2-After PEG mortality rate in 30 days : %3-23 I want to do multiple regression analyses . I have 2 dependent variables "mortality after peg " and "complications after peg" I have 31 independent variables. I do calculate at GPower . F test- Linear multiple regression : fixed model, r2 deviation from zero: A priori Effect size 0.15 , a 0.05 B: 0.95 number of predictors 31 Sample size 264. ***My question is this how can ı find effect size? Do you suggest me any other advice for my research sample size calculate ? Thanks for helping and sorry for baby english and long question.
Hi! As I am not a statistician, I cannot fully help. However, based on what I've understood of your question, you may be able to calculate the odds ratio for the percutaneous endoscopic gastrostomy vs. those without the intervention. This would give you the effect size parameter to input into the sample size calculations. For the regression analysis, perhaps a correlation coefficient would be the best way to assess it's effect. Please confirm theses approaches with a qualified statistician :)
Isolation of CTCs are also another popular use for SepMate/Leucosep tubes.
Good to know, but what does CTC stand for in this context? Thanks.
@@adwoabiotech circulating tumor cells. A popular prognostic biomarker. Look into cellsearch assay.
@@adwoabiotech Circulating Tumor Cell.
Download g*power here: www.psychologie.hhu.de/arbeitsgruppen/allgemeine-psychologie-und-arbeitspsychologie/gpower
Additional Essential Cell Culture Calculations (making up media and determining amount of drug treatment): ruclips.net/video/04y4Dl0uTA8/видео.html
Can a man be a carrier and not even know?
Hi Wendy, Yes, most individuals infected with HTLV-1 remain asymptomatic carriers throughout their lives. Only, a small proportion of infected individuals develop HTLV-1-associated diseases.
Can this virus 🦠 cause CML?
I'm not sure about this one but the two main clinical conditions associated with HTLV-1 are adult T-cell leukemia/lymphoma (ATLL) and HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP).
Excellent ❤
Thanks
Reference 2): Sequencing artifacts derived from a library preparation method using enzymatic fragmentation: journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0227427&type=printable
Reference: DNA Library Preparation Methods for Next-generation Sequencing: Tone down the bias www.sciencedirect.com/science/article/abs/pii/S0014482714000160
Thanks!
You're welcome, mate.
here we join the base with a line manualy which is error prone for the area calculation
Yes, that does sound error prone.
References: 1) High rhesus (Rh(D)) negative frequency and ethnic-group based ABO blood group distribution in Ethiopia: www.ncbi.nlm.nih.gov/pmc/articles/PMC5530478/#:~:text=In%20Africa%20and%20Asia%20the,negative%20phenotype%20is%20even%20rarer. 2) Chapter 2: Blood group antigens are surface markers on the red blood cell membrane by Dean L. Bethesda (MD): National Center for Biotechnology Information (US); 2005. www.ncbi.nlm.nih.gov/books/NBK2264/#:~:text=Blood%20group%20antigens%20are%20either,the%20transfer%20of%20sugar%20units. 3) Blood groups (Better health channel VIC health) www.betterhealth.vic.gov.au/health/conditionsandtreatments/blood-groups# 4) Blood Groups and Compatibilities www.rch.org.au/bloodtrans/about_blood_products/blood_groups_and_compatibilities/
Hi Maame Adwoa, please i have a malaria vaccine to analyse the protein content. I have the Bradford Protein kit from thermofisher and the Albumin standards for the curve. can you help me with how to go about it?
Hello Alhassan, You will need make a 6-point serial dilution of a protein standard for which you know the starting concentration. e.g. Bovine Serum Albumin (BSA). You will then measure the absorbance of that standard against your protein of interest, in this case, your vaccine. If you have a plate reader, it may be also be easier assay it with that instrument (provided you have enough reagent for the required plate reader vol., typically 200ul). I have a video on here, that discusses how to approach protein determination so I'll link it below.
Standard Curve: ruclips.net/video/MPQt5DemzjM/видео.html
i tried to contact them but they will not response
Very nice presentation
Thanks :)
Hi, thanks for the video. Please, I have a question: I will like to ask a question about cell treatment experiment with a test drug for 72 h. In such an experiment, do I have to add my drug once and incubate for 72h then check the cell viability or I have to remove the drug solution and replace with the same drug and concentration after each 24h to avoid wrong readings due to degraded drug in wells or evaporation? Please any other person reading the comments can also reply me if you have the correct answer to my question. Thank you
The usual strategy is to leave the drug on for the entire 72hrs and assess following the designated time. However, it depends on the stability of the drug that you are assessing. If you know that your drug of interest is unstable and is completely degraded after say, 24hrs, then it may be wise to replenish.
Thank you very much for your timely reply. The information will add to my considerations and subsequent decision @Adwoabiotech
hi thank you for your explaination. I have a question, if I measure dose dependency, in specific, I measure basal value of each well for 10 mins, and then add agonist and measure for 30 mins more. Then its supposed to have many points, then how should i choose value to make Z' factor?
I am a complete novice with ELISA but i need to run some blood tests that the UK does not offer, im going to buy all the equipment to conduct the following tests: MSH TGFB1 VEGF MMP9 C3A C4A Can you recommend a good book for the protocols and what elisa tests would be appicable for these tests (dircect/indierct etc.....) The top one is melanocyte stimulating hormone and i need to see a quantity not just sample presence in all of them Thank you, great videos
Hi @Rayshoesmith In terms of whether you perform a direct or indirect test, this will depend on how abundant the target is. If you suspect very low expression levels, then go for indirect. Regarding the melanocyte stimulating hormone where you need to see quantity not just presence, you will need to include a 6 or more point standard curve. This will help you report quantities. Regarding a book: I found this one online: ELISA: Methods and Protocols (Methods in Molecular Biology, 1318) 2015th Edition www.amazon.com/ELISA-Methods-Protocols-Molecular-Biology/dp/1493927418 All the best!
@@adwoabiotech you are an absolute angel! Thank you for taking the time to reply, I really appreciate you ❤️
How do you adjust the gain?
Thank you so much for this practical lecture for university students like me who cannot have the condition and biotechnological facilities to practice. I wish you all the best in 2024, Madam!
Glad it was helpful!
does the volume of the samples have to be constant
Without having more details about which volume you are referring to, I will still say 'yes', because volume affects concentration.
Really helpful thank you !
Happy to hear.
Why do we need to be able to do such calculations? In order to maximize all available storage space most solutions are stored in a concentrated form (known as stock). These solutions are then diluted to the required strength as and when required. This also means the same solution substance may be used for a different range of needed concentrations.
Thanks for the information. Love it
You are welcome @drtomiomoya.
7:36
Great video 👍
Medaase / Thank you.
keep up the goodwork. I hope someone there's multiple of someone like you in this world. You keep the world turning
Thank you! ❤️
You are welcome.
What is a good H-index? Hirsch suggests that after 20 years of research, an h-index of 20 is good! It means that you have 20 publications with at least 20 citations. 40 is outstanding, and 60 is absolutely exceptional.
I analyzed your channel and I was impressed to see your video quality. your video thumbnail is very eye-catchy. However, you are not getting proper views and subscribers. Below we found some problem for your channel - Your video title is not SEO friendly Don't have proper tags and keywords Don't have meta tags for video ranking Descriptions are not optimized Please fix this problems to get more views and subscribers
Ok thanks for letting me know 😆
I struggled to listen to it due to the music which is a shame as sounds like it may have been useful 😢
The music in this video is barely audible. You may have had another browser opened, that was playing music?
@@adwoabiotech Sorry but I do not agree with you. The music is not as low as you say and it makes harder to understand what is being said.
@@albertofabbri1543 Ok, thanks. I appreciate you letting me know. I will keep it in mind for future videos.
Other terms used to describe water quality in the lab includes: MQ/MilliQ water for type 1 water; Elix water for type 2 water and RO or demineralised water for type 3. Note that distilled water is water that is clarified through heating the water until it enters vapour form, and then cooling the water back to liquid form. Most labs do not use this method of clarification, preferring to use reverse osmosis, due to its greater efficiency.
Of course it is obvious that we do not appreciate qualities and the cost that it rather saves.
It seems common sense once you learn it, but before then, it's not intuitive.