The Sanger Method of DNA Sequencing
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- Опубликовано: 25 авг 2024
- This is a short animation detailing the steps involved in the original Sanger Method of DNA Sequencing. I hope you enjoy :) Also please note a mistake I made, DNA synthesis occurs in 5' to 3' direction!!
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When a 3 minute video describes a topic better than a college professor lollll
True😂😂
Sooo true 😂😂🤦🏼♀️
I came Here from my college's professor video 🤣 I couldn't understand what she was saying
ㄹㅇ ㅋㅋ
😂😂😂😂
DNA polymerase synthesizes in the 5'-3' direction, meaning it reads the template strand in the 3'-5' direction. It needs a 3'OH to extend from. In this animation you place the primer on the 5' end which is not right
I was also going to point that out! Good catch!!!!
Yeah thts rgt...I didn't notice it until ur comment....thanks bud
*Note for others watching*
DNA/RNA is synthesized 5'->3' end and template is read 3'->5'. so the primer would have needed to been placed on the 3' end of the template strand. (not the 5' end suggested in the video)
One way I remember this is "5 to 3, I build me" (me and three rhyme.)
"3 to 5, reading is high" (high five)
Hope that helps anyone!
That was really useful
Thank you 💙
This video has an error. The synthesis of DNA occurs in 5' --> 3´direction. The primer should be design to anneal in the 3' extremity of DNA template strand.
Last 15 seconds really made it click for me regarding how the heck the bases are translated from lines to letter sequences. Thanks!
Very good but the primer has to be attached to the 3 prime end of the template strand.
The DNA polymerase in this video is not synthesizing in the right direction, as the video has it polymerizing in the 3' to 5' direction, which isn't correct. The primer should be attaching to the 3' end of the template strand, and polymerase should be acting in the 5' to 3' direction instead.
Miles Gamboa agree the new strand should run anti parallel to the template strand
primer should be added to the 3' end of template which would make it 5'-3' since you need the incoming nucleotides to attack at the hydroxyl....
Exactly...
Since the DNA polymerase only catalyse the polymerisation in 5'-3' direction....
I would like to point out a critical error. The primer is complementary to the 3' end of the DNA template strand, not the 5' end. Polymerization will occur in the opposite direction of what you've shown above.
You cut down an 8 minute lecture on this topic to 3 minutes. With great visuals. And music. I love you.
Primer needs to be on the 3' end not the 5' end. Remember, DNA is synthesized 5' to 3 prime.
It's wrong
Yes you are correct
These short videos are more informational and time saving than school and college classes ......
Nicely explained… but damn i cant believe how much humanity has evolved and advanced.. This is so difficult to even understand and learn i cant imagine how Sanger must’ve invented this … And overcoming the practical challenges in performing the experiment is a whole another challenge
you people saved me.I have my presentation tommorow don't know what i would have done without it..A SINCERE THANKS :)
Once the concept hits.....u will be speechless!
Tue
True
Wonderful animation. I regret, however, that it has an error regarding the position of primers. The primer is placed in the 3´ region of the segment to be used as a template. And the polymerization is in the 5 '- 3' direction of the nascent chain.
So aggressively positive, my nihilistic soul is in AGONY
The Primer is not on the good side at 0:32 and the DNApol goes in the wrong direction 1:20
There is just one little mistake, the primer attaches to the 3' end of the template because it synthethizes from 5' to 3' end.
yeah, the DNA polymerase reads from 5' to 3' which wouldn't work with how they attached the primer in this video
Hi Daniel, I am just watching the video and I notice the same mistake, DNA pol reads from 3’ to 5’, and synthesize from 5’ to 3’. This video is excellent even though has this mistake. They should’ve fix it.
Thank you for this lovely animation. Now I finally understand the Sanger Method better.
I think there's a mistake in primer position it should be on 3' so that the new dna strand would be 5'-3'
thank u for pointing that out
2 errors.
1. Denaturation is by NaOH, heat is primarily used in chemical method of sequencing.
2. Primer binds to the 3' end of the DNA
thank you so much, this was so clear and i understood immediately after being clueless for entire lessons!
The primer was annealead at the wrong side of the template strand in the beginning of the process, it should be at the 3' of the template ensuring that the polymerase can proceed in 5'-3' direction. Overall, you made a really good job with this animation!
Sorry I don't really understand what you're saying? DNA polymerase binds to where the primer and strand has annealed so wouldn't DNA polymerase be moving from 3' end to 5' end when the primer is added at the 3' end?
Nucleotides can only be added to a free 3' end on the forming strand. So when the primer is added to the 5' end of the template strand, that means that the free end of the forming strand is also the 5' end and therefore you can't add nucleotides to it.
For a solid minute I thought I've been lying to myself this whole semester when I saw the DNA being synthesized the wrong way loool. Glad the other comments confirmed the video is wrong in that part.
i am always afraid of being confused by this ends - it is very often mistake in internet)))) And where you studying (if you don't mide to tell)? What your professors said about mistakes of this type?
you made my day. no one cane explain simpler than this
Best video over Sanger's Method of Sequencing I have seen thus far
just the thing I needed after my 2 hour lecture on this topic !
this video makes me think my book is not capable of explaining concepts
Thank you for the video. The only one that actually makes sense
to help you, about the same cooper7e.sinauer.com/animation0408.html
I find it absolutely shocking that you created this excellent animation using only Powerpoint.
Amazing, thank you.
one of the best explanations i've come across
The words got snapped away by thanos
ABSOLUTE BOMB, completely cleared up the concept
The primer should be at the 3' end. The DNA polymerase slides over the template DNA strand in 5'->3' direction
The primer is annealed onto the 3' end
Yes true
👍👍
Never thought that video about fricking molecular biology can be this cute :DDD
could the author of this just put a little annotation correction annotation over the 5' and 3' bit? otherwise it is an awesome video
The Wikipedia pages was Greek to me but this made it so much clearer, thank you!!!
thank you for saving my life..no one can explain it simpler and easier than this....million times thanks
After having a stressful day at the study, that music really cheered me up.
Thanks!
extremely well explained, easy to understand and the animation was well constructed... made it a lot easier to understand. thanks xD
please confirm about the primer annealing , that should be at the 3' end.....so that the extension can take place from 5' - 3'.
Very helpful clearly every step visualise thankyou but I thought only mistake is an primer annealing at 3'end not 5'end because direction of daughter strand dna is 5' to 3' and templet strand direction is 3' to 5'
PRIMER IS ON THE WRONG SIDE SIS
How come ?
It’s labeled 5’
Other than the 3' and 5' ends being labelled wrong this is a good overview, the nucleotides would not be added to the 5' end as it has no 3' OH for the nucleotide addition
Nice! Wish me luck for the exam tomorrow guys!
Good luck man!
So you took the exam today 😌 how was it?
@@earnitdamit961 thanks!!!
@@sarah4373 I crushed it haha 🙌
I mean the time was a little bit short but anyways, went well! Thx!
thank you for this video! i always come back to this when i need a refresher
Thanks for making this. It really helped clarify things
Concise, and precise. Very well done.
Except that the primer should be at 3' end not 5.
GOD BLESS YALLS SOULS AMEN this is exactly what i needed
maybe you should be taking a course on not being a total idiot..
Came across this video before reading the topic. Thank you for the excellent animation and of course explanation :)
One of the best videos about the Sanger Method.
I agree to some people that some info is missing so a newbie cant fully understand this tech.
TOO GOOOD! awesome and mostly simply explained. thank you
the primer will be annealed to 3' strand of DNA not 5'
Yes this part is wrong in this video ,even another important point is missing here ,for binding to the 3' end they need a m13 vector for the binding of primer to 3' end
@@imtiyazalom8850ikr
And i was confused!!! I was Like wait what. Did i learn it wrong all These years??
the song slaps hard
It's very kind and calm, and in the same time shows very clear the ideas of this tecnique.
primer attaching side of template DNA is wrong .........
I'm glad someone else caught that mistake too haha
Very good. A very efficient exhibition of description and visualization has been done.
This is the best video I've found about this subject that explains the basic concept!! Thank you!
Primer always goes to the 3' ,not the 5'
Yep! I made a note of that mistake in the video description :)
Definitely should correct the 5" to the 3" as this tool is useful for revision but this detail is quite significant.
Great video, very simple and straight to the point!
absolutely awesome, thank you so much for a brilliant and simple explanation and fantastic animation!
Nice one! RUclips needs more clearly explained and nicely animated videos about molecular biology
amazing so helpful people!!!!!!!!!!!! even the professor was not able to clarify it that much !! thaaaaaaaaanks
Sanger was such a genius!
easy to learn and understand. Thank u
Doesnt the primer hook on at the 3’ of the single stranded DNA?
the 5' and 3' end are wrongly labeled
I will depend on this video for my next exam
Wow ...this is so good....I learnt the whole two pages topic in 3 mins ...thanks bud💛
Great animation to provide a basic conceptual understanding of the Sanger Sequencing method! Found it very useful, thank you!
I saw in other comments, people have already pointed out that the primer is always designed for the 3' end of the template, not the 5' end, so I'm guessing you guys have already taken note of that. If possible, I request the team to correct that, because newbies could really get confused by that, especially if they are just beginning their journey in molecular biology and don't have a firm understanding of anti-parallel nature of complementary DNA strands.
Overall, great job! Good luck with future videos! :)
Could you recommend book or article where there history of how chemys or physics first time determin this antiparralelity or this theory could only came from James Dewey Watson with Francis Crick (and Rosalind Franklin alone) and their X-ray crystallography or not?
Wow it makes perfect sense now thanks !
pretty sure the rna primer attaches to 3' end of the template strand
madelaine ocampo if it’s on the top wouldn’t it technically be on the 3' side
Lol nvm im dumb!
Dishwasher LOOL it’s okayy
Actually, DNA is readed in 5'-3', and 3'-5', he puts the primer in the 5' in this case the 5' is on the right and the 3' its on the left. :)
I see where the error is, but if in the other DNA template the Primer is attached in the 3' then itll be okay
Thank you, this is really well made and helpful!
this is excellent! double thumbs up!
Thank you! This video is very clear and helpful!
Very very helpful. thank you so so much
Wow... Great explanation just in 3 minutes ..well-done
We have to add primer at 3' prime end of template ryt ,then only we get free 3'OH for polymerization....
great bro 👍🏼
Super clear and easy to understand thank you!
Best animation ever
This is brilliant. Thank you.
Watched this in my Microbiology class! Have my final Lab Exam today!🥲
how did you do??
very very thanks to the creator
it help me a lot next sunday is my test 😊
wish me best of luck 😊
Awais khan DC best of luck
easy to understand and very clear.
Thanks a bunch. brief and understandable 👏👏
Glad it was helpful!
Very informative.. Clear and easy
very helpful! thank you!
Thank you so much for a clear and concise video! Explained much better than my prof
The happy music is nice too.....
well explained . thank you.
This video is soo detailed Thank you so much. As someone else mentioned the words disappear little fast otherwise its great.
this is excellent. thank you!
This was super helpful, appreciate it. thank you
No problem!
awesome. So simple and very easy to follow :)
I finally got it 😄 Thank You!
Bro.
Dna synthesis always starts from 5' to 3'
😅😅😅
You accidentally showed it from 3' to 5'
😅
Yeah, they realized LOl.