Hi David. Could you please tell me, how should I process QCPMG data in TopSpin? My supervisor told me that I need to process QCPMG data in time domain, number of echoes is equal to 6. In time domain, it looks like 6 peaks with decreasing amplitude, the first peak is half-echo signal and the other 5 peaks are echo signals. I need to find the integrals under each peak and fit the obtained points with exponential function to calculate T2 relaxation time. But I don't understand, how to extract the FID (raw data) of QCPMG for processing. The horizontal axis in time domain is in [#fid] units, not in seconds. Is there any common way to process such QCPMG data obtained in TopSpin? I will be very appreciate for your help.
how do I create a text file with chemical shift values as you will find in the experimental section of a paper? I was able to do this in MNova, is there a way to do it with topspin?
Hi there, I tried to use Topspin software to intepret my nmr data. However, when I choose integration button, but I couldn't find delete button to delete all integrations that already done. is it this software problem? or is there any way to delete it?
I show this in the video beginning at 5:40. If you are using a later version of the software you may need to select all the integral versions, then delete. Alternatively, in integrate mode, you can click on each integral and delete them one at a time.
I am assuming you mean that the automatic phase correction did not correct the phasing. In that case you can use manual phasing. Click on the Adjust Phase button, then click and drag (up or down) on the '0' in the phasing line of buttons until the largest peak is phased. Then click and drag (up or down) on the '1' button until the rest of the spectrum is phased.
I am facing problem in nmr analysis. When i select the sample ...i am getting two spectra , one is below and other is in the upper side of ref and i am not able to remove that
I'm not sure what you are describing, but I think it is the active spectrum below, with a full overview spectrum above, with the active portion highlighted in green. If so, there is a button (icon) to the right end of the lower row that can toggle the overview spectrum on and off. If you hover your mouse pointer over the icon you should see a little pop-up that describes the action of the icon (Toggle the display of spectrum overview). Alternatively you can type '.ov' in the command line at the bottom of the screen (don't forget the period before the ov).
Thanks for the explanation. Helps much
Thank you very much. That was very helpful to me
fabulous work!
Life-saving, thanks!
very clear and helpful tutorial
Thank you very much for your explanation Sir.
Thanks for the explanation. Can I change the broad of the peak?
Hi David. Could you please tell me, how should I process QCPMG data in TopSpin? My supervisor told me that I need to process QCPMG data in time domain, number of echoes is equal to 6. In time domain, it looks like 6 peaks with decreasing amplitude, the first peak is half-echo signal and the other 5 peaks are echo signals. I need to find the integrals under each peak and fit the obtained points with exponential function to calculate T2 relaxation time. But I don't understand, how to extract the FID (raw data) of QCPMG for processing. The horizontal axis in time domain is in [#fid] units, not in seconds. Is there any common way to process such QCPMG data obtained in TopSpin? I will be very appreciate for your help.
how do I create a text file with chemical shift values as you will find in the experimental section of a paper? I was able to do this in MNova, is there a way to do it with topspin?
You can't in Topspin. This is a feature of MNova.
Hi there, I tried to use Topspin software to intepret my nmr data. However, when I choose integration button, but I couldn't find delete button to delete all integrations that already done. is it this software problem? or is there any way to delete it?
I show this in the video beginning at 5:40. If you are using a later version of the software you may need to select all the integral versions, then delete. Alternatively, in integrate mode, you can click on each integral and delete them one at a time.
What would you recommend I do if the dips still show up after the automatic correction?
I am assuming you mean that the automatic phase correction did not correct the phasing. In that case you can use manual phasing. Click on the Adjust Phase button, then click and drag (up or down) on the '0' in the phasing line of buttons until the largest peak is phased. Then click and drag (up or down) on the '1' button until the rest of the spectrum is phased.
I am facing problem in nmr analysis. When i select the sample ...i am getting two spectra , one is below and other is in the upper side of ref and i am not able to remove that
I'm not sure what you are describing, but I think it is the active spectrum below, with a full overview spectrum above, with the active portion highlighted in green. If so, there is a button (icon) to the right end of the lower row that can toggle the overview spectrum on and off. If you hover your mouse pointer over the icon you should see a little pop-up that describes the action of the icon (Toggle the display of spectrum overview). Alternatively you can type '.ov' in the command line at the bottom of the screen (don't forget the period before the ov).
Es solo para computadoras de 64bit?
How do I get the topspin app
Please I need Bruker TopSpin software DOWNLAD LIN