Both will work, although 1500X will only look bigger, but not clearer. The limit of light resolution through a light microscope is about 1000X magnification.
@@sci-inspi Why isnt the crystal violet washed away with the water. it hasnt been mixed with the iodine yet to form the complex that's insoluble in water.
@@aaronrichardtalley The water cannot penetrate the cell since it is a polar solvent. Therefore it only washes away crystal violet that hasn't penetrated the cell. This is also the reason that alcohol (a nonpolar solvent) is used for the decolorisation step and not water. In fact, even the alcohol can only penetrate those cells that do not have a thick peptidoglycan layer. In that case, the crystal violet is not washed away, and that is how/why this Gram stain procedure works.
Gram + bacteria have a thick peptidoglycan layer that binds to the crystal violet-iodine complex that doesn't wash off with grams alcohol. so Safranin can't stain it since the CV-I complex is already there. Gram - bacteria don't retain the CV-I complex after washing with grams alcohol. so it is stain with safranin.
There are different decolourisers used in Gram staining. Acetone can bleach the dye somewhat, but alcohol will not. So, it depends what decolouriser is used.
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can i play this music during my lab practical tmrw??
Lmao😁
😂
Can I smile like this 😀 after 5 years
The examiner will groove along with you! 👍
This my exam today I'm happy to see that I did it well as required. Thanks for posting.
A lot of thanks, very good video, please DO MORE VIDEOS!!!
What magnification should be used when observing a gram stain? 1000x or 1500x
Both will work, although 1500X will only look bigger, but not clearer. The limit of light resolution through a light microscope is about 1000X magnification.
Very interesting video. Why aren't the bacteria washed off during any of these steps? Does the "heat fix" keep them in place? Thanks!
Correct, the heat fix step sticks them to the slide.
@@sci-inspi Thanks for the quick reply.
@@sci-inspi Why isnt the crystal violet washed away with the water. it hasnt been mixed with the iodine yet to form the complex that's insoluble in water.
@@aaronrichardtalley The water cannot penetrate the cell since it is a polar solvent. Therefore it only washes away crystal violet that hasn't penetrated the cell. This is also the reason that alcohol (a nonpolar solvent) is used for the decolorisation step and not water. In fact, even the alcohol can only penetrate those cells that do not have a thick peptidoglycan layer. In that case, the crystal violet is not washed away, and that is how/why this Gram stain procedure works.
But we don't use blot paper to dry it, we air dry the slide
That works too. Some lab techniques differ lab to lab.
Learned and enjoyed watching 😀
Very interesting video sir but sir I have one confusion that the smear will be prepare by the process of heat fix?
Good job useful video 👏🏽👏🏽
very helpful video thanx...and next time explain mechanism of the Gram staining...good luck
See 2:05 for the mechanism.
Wow
Thank you 😊
I love it
Why aren't Gram+ stained by safranin as well?
Gram + bacteria have a thick peptidoglycan layer that binds to the crystal violet-iodine complex that doesn't wash off with grams alcohol. so Safranin can't stain it since the CV-I complex is already there. Gram - bacteria don't retain the CV-I complex after washing with grams alcohol. so it is stain with safranin.
How long do I heat fix the slide for?
About as long as was shown in the video. It's usually only two slow passes over the flame.
Short n simple... Good
Can i use cover clip
Yes, a coverslip can be used.
bruhh the electric guitar is legit😂😂
Meri slides nahi dikh thi
Thanks you, very nice Video
Thank you!!!
Very cool!
don't you have to put acetone for less than a second? or does it matter?
This protocol says 10-20 seconds. I have gram stains that come out fine following this protocol.
No need
There are different decolourisers used in Gram staining. Acetone can bleach the dye somewhat, but alcohol will not. So, it depends what decolouriser is used.
Very nice
Awesome ☺️
Gram + ve bacteria is coccus and Gram -ve bacteria is rod shaped👍
Gram positive stains purple, and gram negative stains pink. Both include cocci and rods. Anthrax, for example, is a Gram positive rod.
A lot of thanks
Fantastic
Thank u
forgot the wax ring and water droplet
good
Staphylococcus 💜
Better than danish grams
my exam is tommorw
👍
nice but we didnt need so much jimi hendrix in thebackround
I beg to differ