Pyruvate Dehydrogenase Complex - Dr G Bhanu prakash , Biochemistry High-yield Usmle step 1 lecture

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Pyruvate Dehydrogenase Complex
The pyruvate dehydrogenase complex links glycolysis to the TCA cycle (also known as the Krebs cycle or the citric acid cycle). It is a large multi-enzyme complex composed of three enzymes involving five cofactors. The pyruvate dehydrogenase complex oxidizes pyruvate to generate acetyl-coA.
Introduction
The oxidation of pyruvate occurs in the mitochondria of the cell. The mitochondria is an organelle in the cell. It is considered the "powerhouse" of the cell. Pyruvate is transported there via pyruvate translocase. Pyruvate dehydrogenase is a multi-enzyme complex that uses three enzymes:
E1: Pyruvate dehydrogenase which uses thiamine pyrophosphate (TPP) as its prosthetic group.
E2: Dihydrolipoyl transacetylase which uses lipoamide and coenzyme A (also known as coASH) as its prosthetic groups.
E3: Dihydrolipoyl dehydrogenase which uses flavin adenine dinucleotide (FAD) and nicotinamide adenine dinucleotide (NAD+) as its cofactors.
Note: Prosthetic groups are molecules that are covalently bonded to an enzyme. The net reaction of converting pyruvate into acetyl coA and CO2 is:
2pyruvate+2NAD++2coA→2acetyl coA+2NADH+2CO2
2pyruvate+2NAD++2coA→2acetyl coA+2NADH+2CO2
The Process
This is a five step process.
Step A: Pyruvate is decarboxylated by pyruvate dehydrogenase with help from TPP.
Step B: The reactive carbon (between the N and the S of the five membered ring) of the TPP is oxidized and transferred as the acetyl group to lipoamide (which is the prosthetic group of the dihydrolipoyl transacetylase). This forms hydroxyethyl-TPP. An H+ ion is required for the intermediate to give off CO2.
Step C: E2 (dihydrolipoyl transacetylase with cofactor lipoamide) oxidizes hydroxyethyl- to acetyl- and then transfers acetyl- to CoA, forming acetyl-CoA.
Step D: Acetyl CoA was made in the previous step. However, the process is incomplete. The E2is still attached to the acetyl CoA molecule. So, E3 (dihydrolipoyl dehydrogenase) oxidizes the thiol groups of the dihydrolipoamide back to lipoamide.
Step E: As a side reaction, NAD+ becomes reduced to NADH.
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