Learn how to import non-Chromeleon data and perform general processing steps such as peak integration, component table generation, and calibration plot creation.
Thank you for this great video. I have a doubt which goes like this: My sodium cation and chloride is eluting at almost similar retention times and also its happening with nitrate and potassium. As i have them in stock, I have made calibration curve with all of them and in my calibartion studio window, i have deleted the peaks of sodium and potassium from all the concentrations because for me chloride and nitrate are more important. However, now when i check my unknown sample and also known sample, the cation peaks which i have deleted are now coming named as chloride and nitrate now..... What should i do.... the permanenet solution which i feel is to make a calibration standard only with the elements you are interested in and which elutes at distinct rentention times. please suggest?
@yaminimittal8182 what is your diluent, flow rate, and column? Are you able to export your sequence and send my way? Feel free to connect on LinkedIn if you want to keep details private.
Good afternoon I would like to know how the signal-to-noise ratio is calculated in crhomeleon for the purposes of calculating the detection and quantification limits. Thank you so much
I am following the procedure- 1. First creating variable in the injection list for sample weight (batch specific) and others (fill weight or average weight). Putting their values before run. 2. Secondly i am creating processing method where two variable created for each component and putting values ( standard weight and standard potency, factor = conversion factor). 3. In reporting template creating formula by calling created variables. Then reporting with unit as required. 4. For next analysis i copied the variable and formula from same sequence and done. Is it ok?
Very helpful video. Thanks a lot. Sir can u please share how can we plot calibration curve using multiple injection results. As for example I hv chromatogram for 10pppm, 20pppm, 30ppm, 40ppm, 50ppm. Now I want to draw the calibration curve for this
When creating a new processing method on its own, how can I add additional UV-channels? All integration settings apply to all channels and I can't add more channels
Thank you very much Joel to post this video. It is definitely very helpful for first time user!
Thank you very much for this excellent opportunity to understand how to proceed to process data !
Wonderful elaboration of each and every single parameter
This is really really informative. Thank you so much for sharing.
Nice tutorial. It would be nice if you would upload some more information about chromeleon. Especially some more info about calibration,
thank you for sharing for knowledge for free! can you do a name peak video.
Thank you Joel, very helpful!
This was so helpful, thank you so much!!
Muy bien video,saludos desde Mexico
Pls add more chromeleon related videos
Great job. What about the reporting video. Is it out yet?
2:50 Import data
7:09 Processing data
20:18 Processing method
27:03 Quantitative method
28:08 Start integration
29:09 Run Cobra Wizard
thank you for sharing the time stamp 🙏
Superb!
Thank you for this great video. I have a doubt which goes like this: My sodium cation and chloride is eluting at almost similar retention times and also its happening with nitrate and potassium. As i have them in stock, I have made calibration curve with all of them and in my calibartion studio window, i have deleted the peaks of sodium and potassium from all the concentrations because for me chloride and nitrate are more important. However, now when i check my unknown sample and also known sample, the cation peaks which i have deleted are now coming named as chloride and nitrate now..... What should i do.... the permanenet solution which i feel is to make a calibration standard only with the elements you are interested in and which elutes at distinct rentention times. please suggest?
@yaminimittal8182 what is your diluent, flow rate, and column? Are you able to export your sequence and send my way? Feel free to connect on LinkedIn if you want to keep details private.
thank you so much, it was wonderful.
I just did not understand the level part in 13 min. why some level will be 1 the other will be 2?
can you please make video on calibration of ic soft ware is chromeleon
Good afternoon
I would like to know how the signal-to-noise ratio is calculated in crhomeleon for the purposes of calculating the detection and quantification limits.
Thank you so much
I am following the procedure- 1. First creating variable in the injection list for sample weight (batch specific) and others (fill weight or average weight). Putting their values before run. 2. Secondly i am creating processing method where two variable created for each component and putting values ( standard weight and standard potency, factor = conversion factor). 3. In reporting template creating formula by calling created variables. Then reporting with unit as required. 4. For next analysis i copied the variable and formula from same sequence and done. Is it ok?
Very helpful video. Thanks a lot.
Sir can u please share how can we plot calibration curve using multiple injection results. As for example I hv chromatogram for 10pppm, 20pppm, 30ppm, 40ppm, 50ppm. Now I want to draw the calibration curve for this
and is there any video about assay test and RSD calculation?
When creating a new processing method on its own, how can I add additional UV-channels? All integration settings apply to all channels and I can't add more channels
Thanks 💜💜