3. UCSC Genome Browser Tutorial: BLAT
HTML-код
- Опубликовано: 10 сен 2024
- UCSC Genome Browser Tutorial
Video 3
A tutorial for BLAT, a search tool that maps nucleotide sequences to the human genome. Here I discuss:
--how to check PCR primers with BLAT
--how to visualize sequencing results with BLAT
--supplementary nucleotide abbreviations
I found all your tutorials on UCSC Genome Browser very useful. Thanks Sam!
+Loredana Peca Glad I could help, Loredana. Best of luck in all of your scientific endeavors!
Hi Sam. Thanks a lot for posting this video - it really helped me with an assignment.
thanks sam
Can I use blat to find exactly to which exons of a gene a probe sequence maps
Hi Sam, I watched your video and it was super helpful! Thank you very much! You also talked about the algorithm behind BLAT in the video, do you have more information about it? I have to write a paper on BLAT but haven't found any good papers yet.
Thanks in advance.
Kind regards
Male
Thanks Sam. Can BLAT search for primers designed for the bisulfite converted sequences?
To BLAT the reverse primer you used the reverse sequence or reverse complementary?
Reverse complement because BLAT expects all input sequences to be 5' -> 3'. Hope this helps!
@@samallon9492 It did. Im working on this right now. Thanks!
can UCSC genome browser used to see the change in distribution of reads
at different labeling times with annotation?
+Farha shazmeen Hey Farha! It sounds like you want to upload a custom track for that task. Did you check out my 4th video? That might put you on the right track (no pun intended). Good luck!