Does anyone synthesis the complete 25 mer oligo first, then print that onto the glass? I could imagine this would allow for precise quality control of the oligos because you could varify the sequence with mass spec prior to printing.
That probably would be very difficult for chips with many unique sequences. Some chips can over 700,000 different oligo sequences (e.g. SNPs) and preparing and keeping track of them and then delivering them to the chip location I think would be logistically difficult so in situ synthesis at each location is apparently the more efficient way to prepare the chip.
This is excellent explanation, couldn't find any info how DNA is fabricated until now.
Ok this is all super mind blowing and this video was very helpful in explaining it all in a straightforward manor
Nice summary - I didn't know how these were made.
Very well explained, thank you
best video about microarray chip manufacture ever!
Billions of likes and thumbs up.
Very nice! Helped me clear my doubts. Thank you :)
Does anyone synthesis the complete 25 mer oligo first, then print that onto the glass? I could imagine this would allow for precise quality control of the oligos because you could varify the sequence with mass spec prior to printing.
That probably would be very difficult for chips with many unique sequences. Some chips can over 700,000 different oligo sequences (e.g. SNPs) and preparing and keeping track of them and then delivering them to the chip location I think would be logistically difficult so in situ synthesis at each location is apparently the more efficient way to prepare the chip.
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