Analytical Instrumentation 06: Fluorescence & Phosphorescence Explained | Learn under 5 min

Welcome to Episode 6 of our "Analytical Instrumentation" series! 🔬✨
In this concise 5-minute animated video, we delve into the basics and principles of Fluorescence and Phosphorescence measurement-essential techniques in qualitative and quantitative analysis within research laboratories, QC labs, and the field of analytical chemistry. Whether you're a student, researcher, or laboratory technician, understanding fluorescence and phosphorescence is crucial for accurate and efficient analytical work.
📚 What You'll Learn in This Video:
Introduction to Fluorescence & Phosphorescence:
Definitions: What do fluorescence and phosphorescence mean?
Common Questions Answered:
What does fluorescent mean?
How does fluorescence work?
How does fluorescence spectroscopy work?
How does phosphorescence occur?
Energy Level Diagrams:
Molecular Energy Levels: Understanding different levels of molecular energies.
Singlet & Triplet States:
Singlet State: Electron pairing and spin configurations.
Triplet State: Differences from singlet states and their significance.
Electron Pairing & Spins: How electrons pair and their spin orientations in different states.
Mechanism of Fluorescence & Phosphorescence:
Fluorescent Emissions: How electrons return to the ground state, emitting light.
Phosphorescent Emissions: Delayed emissions due to triplet state transitions.
Energy Transfer Processes: How energy levels facilitate light emission.
Applications in Analytical Instruments:
Spectrofluorometer: Introduction to the instrument used for fluorescence and phosphorescence measurements.
Qualitative & Quantitative Analysis: Using emitted light to determine analyte concentrations.
Comparative Analysis:
Differences Between Fluorescence & Phosphorescence:
Emission Lifetimes: Immediate vs. delayed emissions.
Energy Transitions: Singlet vs. triplet state transitions.
Applications & Advantages: When to use each technique for optimal results.
🔍 Key Topics Covered:
Fluorescence and phosphorescence fundamentals
Energy level diagrams and molecular states
Mechanisms of light emission
Spectrofluorometer operation
Beer-Lambert's Law in quantitative analysis
Comparative analysis of fluorescence vs. phosphorescence
🎯 Target Audience:
Students and professionals in analytical chemistry
Researchers and laboratory technicians
Individuals interested in the mechanics of analytical instrumentation
Anyone looking to deepen their understanding of scientific instrumentation and analytical techniques
Stay calm and keep learning! Whether you're a student, researcher, or enthusiast, this tutorial will equip you with the knowledge to master fluorescence and phosphorescence measurements effectively-all through engaging 2D animations!
🔔 Don't forget to like, share, and subscribe for more insightful and educational videos on analytical instruments and engineering concepts!
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[@PracticalNinjas]

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[@bosong2515]

I really like the video but I would like to suggest that you remove the background music. It is distracting!

[@PracticalNinjas]

Thanks Bo Song for your valuable suggestion. We are glad you liked the video. We will try to remove the background noise or atleast reduce it's volume level in our future videos :)

[@vrajpatel429]

No back ground music is good I like it it effective

[@Chemvocalist]

Exactly,why the hell is background music there

[@jermiinus]

@@PracticalNinjas Would you please reupload this video without the background music?

[@PracticalNinjas]

Due to certain technical issues we are unable to upload the video without background noise

[@vinayegalapati2191]

You're explanation and PPT was awesome 😍
But, Please decrease background volume
I'm unable to concentrate on the concept

[@soumyamuttagi]

How I couldn't find this video earlier. Really amazing and beautiful explaination. Finally I can score and understand at the same time 🙌🙌🙌thank youuu

[@Alex-vs8ev]

Man, I'm from Germany. And idk what the hell is going on with our teachers but I understood this topic better in not even 5 minutes than in 2 weeks of school lessons. And your video is in English !!!

[@TestEric]

WOW, amazing job, this is exactly what I was looking for. I'm shocked more people are not interested in this topic.

[@cherryberry6492]

Good explanation.But music is make concantrate difficult.

[@PracticalNinjas]

+cheery berry. Thanks for the feedback. Will try to keep music volume low in the next videos 😃

[@cherryberry6492]

Thank you for your consideration :)

[@PracticalNinjas]

Hope you enjoy other videos on our channel as well :)

[@ACswe91]

Yes keep the music but little lower :)

[@PracticalNinjas]

Sure ACswe91 :)

[@pritykarmakar4937]

Very helpful video, thank you so much sir 🙏🙏🙏😊😊😊

[@hceperepnoname8752]

Cool!Thank you so much!

[@minhavidavai]

This was great. I needed to remember these concepts in short time. Thanks

[@ncarti1601]

Great video, really liked the visuals and it helped me understand it more!

[@chitrakeshwani4047]

thank you so much for making this video you have explained this phenomenon in a very simple and comprehensive way

[@PracticalNinjas]

Thanks Chitra Keshwani for such a positive feedback. We are glad that you liked the video!!!

[@LadyShaydex]

I think you should add why fluorescence light is higher in wavelength, due to energy being lost to vibration

[@gameon4195]

Higher in wavelength dear please correct

[@LadyShaydex]

@@gameon4195 Lower in wavelength to the incident light is what I meant

[@LadyShaydex]

@@gameon4195 Oh wait you're right

[@gameon4195]

@@LadyShaydex thanks 😊

[@الجبلالموصلي]

The 1st second I was like "music😫", but then I was into the mood,thank you so much, I was having real trouble with singlet and triplet state 🌺

[@muhammadshuaib406]

very helpful for beginner

[@sowjanyaponnada7234]

Nice and telugu explanation... Is better to understand

[@safwanahrahman4109]

simple explaination and i understand the whole thing better!!! THANKYOU

[@PracticalNinjas]

Thanks Safwanah Rahman for such positive words.

[@HamnaAzhar7]

Beautifully explained... Thank u for making it quite easy to understand!

[@amitvele]

Excellent work. Colorful and creative. Makes the video engaging.

[@PracticalNinjas]

Thanks +Amit Vele.

[@jazmingarcia6566]

Such a good explanation. Music is a little too loud, but great video. Thank you so much!

[@harshitabhati4747]

Awesome!! I can never forget the concept after watching this!!

[@PracticalNinjas]

Glad you liked the video Harshita Bhati :)

[@innocentchemist2556]

Good work. it was really helpful for me. this video cleared my basic concepts!!

[@nishadkaippally8247]

The background music adds so much meaning to this presentation. Perhaps more volume could enhance our understanding.

[@pratikadarsh6894]

Very helpful. Thanks

[@bugzy9476]

Good explanation..keep it up

[@savitact7960]

thankyou. very well explained. it would me better if no background music.

[@PracticalNinjas]

Thank you +Savita Ct for the support and feedback. The background music in the future videos will be kept to a minimum so that it does not distract the viewers from the main concept :)

[@yasinbaraqouni9395]

Agree with you

[@leif1075]

@@PracticalNinjas why is it called triplet state instead of doublet which makes more sense since there are two?

[@leif1075]

@@PracticalNinjas Thanks for the video. I actually liked the background music..Could you tell me who the composer is?

[@tejasshewale953]

This explanation is really beneficial ...

[@sumonagarg3428]

Very helpful Thankyou so much

[@bushra6319]

Best 👍 jazak Allah Khair

[@betneha]

Thank you for explaining Jablonski diagram.

[@annurukay7040]

Asome sir u define it very easy to understood 👌👌

[@ELLE-zr3cs]

awesome music!and explanation

[@3ashmawy660]

useful and easy to understand explain .
thanks

[@vrajpatel429]

Very nice discription very good way to explained keep it up

[@mahnoor6216]

Thanks for clearing out concepts 😊

[@abhiriseagain9303]

Thank you so much❤️❤️

[@laszlokecskes8072]

well done! thanks!

[@tehminashafique74]

It was so simple and easy to understand thank u

[@mohanrommala8252]

really simplified explanation

[@mdasifansari2061]

Very nicely explained 👍👍.... Thank u so much

[@torlarsen2212]

Amazing video sir! Thank you so much

[@kashishkanodia5629]

Thankyou for the simplified explanation!

[@dariyatukhmetova1172]

this video is amazing, thank you vvery much

[@malaneyy408]

This was so good thank you. Agree with others about removing the music though.

[@Smoothcurveup52]

Thank you so much sir
Superb explaination

[@dwipayanhalder4388]

Good explanation

[@kiranyadav9782]

Thanku soooo much sir 🙏🏼🙏🏼🙏🏼🙏🏼🙏🏼

[@vineetkhantwal9410]

Awesome 🤗🤗🤗

[@kimsd6269]

thanks you for your efforts, Indeed.

[@nerym.aguilar4297]

You are amaziiiiiiiiing!!!

[@BossRotah]

Great content

[@pdiala2]

Perfect explanation. Everything I was looking for

[@PracticalNinjas]

Glad we could help you :)

[@rabiyanoor4459]

Tremendous explanation... Helped me alot

[@missdaydreamer6777]

Helped me a lot. THANK YOU! Suggestion though, remove the effect on the texts. :)

[@PracticalNinjas]

Thanks a lot for your feedback :)

[@Gopi_6094]

supper...b i really like this video

[@RaniYadav-gx4yt]

Nice video sir

[@sukanyaghosh6503]

Beautifully explained!!!

[@PracticalNinjas]

Thanks Sukanya! Glad you liked it.

[@shardanaa4967]

great video man

[@MARVIW]

hallo

[@MARVIW]

im big fan

[@saadrashidusmani832]

love the video maker

[@chaski315]

Overall this is a great video. Thank you!

[@KR-jz9xv]

Thank you so much for a very clear and straightforward explanation!

[@PracticalNinjas]

We are glad you liked our video Khawllah Roussi

[@19arathiv49]

thank u so much

[@preetikhobragade1952]

Best video ...

[@PracticalNinjas]

Thanks preeti khobragade

[@sjoerdklomp3931]

Thank you this helped a lot i have to make a oled from Ru(bpy)32+ and this helped me learn some of the basics

[@PracticalNinjas]

Happy to hear that the video was helpful :)

[@paritoshsharma2841]

Thanks a million !!!! Nice explanation . Keep it up guys 👍

[@PracticalNinjas]

Thanks Paritosh Sharma for the support :)

[@cartikey3743]

@@PracticalNinjas but bro my question is why that Intersystem crossing takes place

[@calvinms7503]

QUESTIONS :
1. Why triplet states has lower energy than singlet states?
2. What can cause intersystem crossing?
3. What can cause the spin change its direction from S1 to T1, from T1 to S1?

[@rixonrainold1193]

1. The answer might sound weird. But the truth is in Singlet state the repulsion between electrons is higher than in Triplet state. Yep it's confusing. Afterall how can +1/2 & -1/2 charges have more repulsion than two +1/2?
Triplet state provides degenerate orbitals, meaning a larger area to accommodate the electrons. In this space similar species(two +1/2) can exchange energies due to the property of degeneracy. Where as in case of a Singlet state the two electrons are stuffed in a single orbital resulting in high repulsion. Now the most interesting part, all these signs + and - are used to denote the orientation of the spin, not the charge. The charge of the electron is always NEGATIVE. Hence very simply, two negatively charges substances(e-) stuffed in a single orbital is highly repulsive and have more energy that two e- in degenerate orbitals.

[@rixonrainold1193]

2. Intersystem crossing (ISC) is a radiationless process of switching orbitals. It is often undergone by heavy atoms. Due to the presence of degenerate orbitals(T1 state) having lower energy (as discussed earlier) the electrons move from S1 state to T1 state.

[@rixonrainold1193]

3. Bro S1 and T1 are not spin directions. They're energy levels(S1 state and T1 state). And the transition of electrons from S1 to T1 is due to the availability of degenerate orbitals (discussed ealier). The change in spin orientation is necessary. Only if their spins are parallel, i.e. two +1/2 or two -1/2, they can exchange energies, and only if that happens they can be accommodated in the degenerate orbitals.

[@somebody9824]

Voice of narrator is really good

[@3wordsoxforddaily786]

thank you so much Now I understand

[@PracticalNinjas]

we are glad the video could help you :)

[@01244070178]

Nice video sir. Excellent explanation 👍

[@kundaiashleychakona1565]

This helped me a lot! Please edit out the music.

[@satyanarayandubey5581]

Nice and superb

[@PracticalNinjas]

Thanks a lot.

[@sidewaysfcs0718]

The Jablonsky diagram doesn't depict orbital energies, but the energy states of the molecular electronic system as a whole.
You cannot put electrons on this diagram like you would in a molecular orbital diagram.
Basically, every energy level in a Jablonsky Diagram represent its own personal molecular orbital diagram which is omitted.

[@snehithatalla7018]

Gud job ...keep going👍🏼

[@PracticalNinjas]

Thanks +snehitha talla. Glad you liked our work.

[@atvoriginals]

_Awesome_

[@S.K-b4n]

Perfect

[@hariom9328]

I appreciate the efforts of making this video with intention to simplify the concept. However, i noticed that you have forgotten to mention several things including the internal conversion, types of molecules that can fluoresce etc. Another thing is that you have wrongly mentioned the lifetime in the singlet excited state as varying from 10^-8 to 10^-4 seconds. These l lifetimes are I few nano seconds only.

[@yasaine7896]

music volume down or a more no music would be better, but excellent video and very easily understandable, awesome job.

[@PracticalNinjas]

Thanks +Yasmine Bingei for the feedback :) Will surely try to reduce the volume in the next videos. Glad you liked the video.

[@Rajput0818]

Nice one thanks

[@PracticalNinjas]

:)

[@meerasubu1788]

Great explanation, I ave a quert why is excitation of radiation at 90 degree

[@imranabbas4067]

loved itt.....

[@sumonroy3766]

Is it molecular transition?

[@faisalwighral8647]

very well explaind

[@PracticalNinjas]

Thanks Faisal Wighral

[@danieldemabur6952]

awesome

[@nallasurenderreddy6355]

Thank u
Helped a lot

[@ketitzudi8495]

understandable.Keep up the good work

[@PracticalNinjas]

Thanks +ketiyala tzudi. Glad you liked it

[@sheikhfahmi6810]

nce vedio thanku

[@PracticalNinjas]

We are glad you liked our video.

[@lonehumi8350]

Thanks a lot sir

[@PracticalNinjas]

Glad you liked it

[@siddhiborkar573]

Plz don't put background music

[@Alex-hh9tw]

music is too loud

[@huzaifaakbar7354]

Good explanation plz avoid music

[@jesper1320]

does this jumping on and off of energy states mean changing orbitals ? or something different ?

[@bablykhatun3928]

Good content but In study videos try not to use background music

[@prakashjoshi6796]

Hi, Where do you make this videos, in which software

[@mauroariascontreras9284]

Nice video man =) and I din't have troubles with the background music...
actually I found it nice hahah poor little children crying

[@PracticalNinjas]

We are glad you liked the video :)

well done thanks.

[@PracticalNinjas]

Thanks +Furkan CAPRAZ. Glad you liked the video.

[@nutansingh5194]

Plz tell about atomic absorption spectroscopy

[@PracticalNinjas]

We had uploaded a video on the topic you asked. But due to some technical doubts we have pulled that video down. Once those doubts are cleared, we will publish the video again.

[@m.skumar147]

superb..

[@PracticalNinjas]

Thanks +Shravan Kumar Muppidi. Glad you liked the video.

[@gurralamohankumar5838]

What is meant by stray Light ??? Plz tell me anyone

[@PracticalNinjas]

The unwanted light in a particular experiment is referred to as stray light.

[@mdkhabeerazhar216]

thanks

[@PracticalNinjas]

Glad you liked the video.

[@raeesladduawesomebhaiya1429]

Thanks sir