Troubleshooting the Bio-Plex® 200 Multiplex Immunoassay System: HTF Alarm

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Learn how to resolve a fault in the Bio-Plex sheath fluid delivery system indicated by the high-throughput fluidics (HTF) alarm. The most common fault is fluid overfill, which is easily remedied by draining and refilling the HTF.
The Bio-Plex system uses magnetic beads coupled with unique fluorpohores and customized detection antibodies for the quantitation of multiple proteins, peptides, and nucleic acids. Bio-Rad offers Bio-Plex assays for cytokines, growth factors, signal-transduction molecules, and disease biomarkers for inflammation, autoimmune disorders, cancer, diabetes and heart disease.
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