I pay for a college course and your a better professor than what I currently have. I've been teaching myself and your videos have been so helpful! thank you!
I searched this topic so much and finally found this video. Thanks a lot... Only gram staining is much talked about but you made me help endospore staining ❤️
Deon Murray Some species of bacteria (but not all) go into an endospore state when conditions for growth become really unfavorable and, yes, high temperatures are one such condition.
No... Endospores form only when bacteria are in very harsh conditions... Just like lack of nutrition..(This stage is a survival strategy) Heating just kill the bacteria.. Hope you understand...
I love your videos, but I think this one should be using Malachite Green and Safranin to identify endospores. The one you are describing here is the Acid-Fast stain. Or am I wrong?
Yes, the Moeller endospore stain uses the same stains as the acid-fast stain. There is a longer "steaming" step between dyes than in acid-fast, but they are pretty similar! en.wikipedia.org/wiki/Moeller_stain
Hi I'm a high school student and I'm trying to do this experiment (endospore staing) and i got problems buying malachite green soultion. So the point is do u know where to buy it or how to make it? And one more thing do u know the difference between malachite green and malachite green oxalate??? Fast answers would really help me thanks !!!!!!
Your videos are great and easy to understand. However, you have a mistake in this video. In the Schaeffer-Fulton method, you do not decolorize with acid alcohol. I would adjust your video to reflect this, as the way it is described sounds like both methods require the destain.
I pay for a college course and your a better professor than what I currently have. I've been teaching myself and your videos have been so helpful! thank you!
You explain stuff so well with the sweetest voice ever.thank you so much you've helped me a lot.
I searched this topic so much and finally found this video. Thanks a lot... Only gram staining is much talked about but you made me help endospore staining ❤️
calm. concise. clear. I love all your lectures on biology! thank you
love your videos! I got a micro lab midterm on monday and these helped sooo much ( :
Nice video, i definitely hats of ur talent , the way u explains the topics really amazing ❤️
Your video is very very best mam
Brilliant is she, I like her way in teaching
Deon Murray Some species of bacteria (but not all) go into an endospore state when conditions for growth become really unfavorable and, yes, high temperatures are one such condition.
Thank You
You are wonderful. Thank you for the help.
your videos are fairly useful and understandable , thank you so much
You're a wonderful teacher !
I'd like to know if heating the bacteria would put it in an endosporic state?
No... Endospores form only when bacteria are in very harsh conditions... Just like lack of nutrition..(This stage is a survival strategy)
Heating just kill the bacteria..
Hope you understand...
Nice explanation than our college teacher explanation.
you are the best!! Thanks!!
Still alive ❤️❤️❤️
Very very helpful !!
Hello, is it preferred to get the sample being placed on the broth be from a slant or a broth?
I don't think it matters.
Thanks so much
Awesome video. thanks
I love your videos, but I think this one should be using Malachite Green and Safranin to identify endospores. The one you are describing here is the Acid-Fast stain. Or am I wrong?
I go over that at 4:30. That’s the Schaefer-Fulton method.
Biology Professor Sorry I meant when it is heat fixed to the slide.. wouldn't that put all the bacteria in an endosporic state?
I'm a little confused as to how this is different from the acid-fast stain. Doesn't that also use carbolfuscin and methylene blue?
Yes, the Moeller endospore stain uses the same stains as the acid-fast stain. There is a longer "steaming" step between dyes than in acid-fast, but they are pretty similar! en.wikipedia.org/wiki/Moeller_stain
Interesting.
Do bacteria decompose? Why are we able to keep slides for so long without them breaking down?
Yes, bacteria decompose. Microscope slides last a long time because the bacteria are “fixed” or “preserved” in some way.
Hi I'm a high school student and I'm trying to do this experiment (endospore staing) and i got problems buying malachite green soultion. So the point is do u know where to buy it or how to make it? And one more thing do u know the difference between malachite green and malachite green oxalate??? Fast answers would really help me thanks !!!!!!
Your videos are great and easy to understand. However, you have a mistake in this video. In the Schaeffer-Fulton method, you do not decolorize with acid alcohol. I would adjust your video to reflect this, as the way it is described sounds like both methods require the destain.
Mam please teach in hindi or simple words
Time for ather stage?
I thought the primary stain was MALAQUITE, and the SAFRANIN?
Would normal home detergent work?
I really don't know. I guess you can try it and see... Good luck!
Hello mam
u r lovely
Came here to learn about staining.......now completely distracted by how beautiful you are. Not getting any studying done now :p