Gotta love how with quarantined schooling teachers have given up on teaching and recognized that people like you have been the ones actually teaching us.
Even with a biology degree and in medical school, this is the most straight forward and easy to understand video on DNA replication. You sir are the GOAT.
I love that I can learn by watching videos. I am interested in many subjects and my idea of a good session is learning something new. I will definitely be checking out Professor Dave!
I just realized something very important in terms of how to interpret your animation. The directionality of the synthesis of the new strand is correct. However, I initially thought that the numbers you put on the leading strand was identifying the ends of the new strand, not the original strand. In other words, the new strand would read/synthesize as such: 3' under the 5' of the leading strand and 5' under the 3' of the leading strand with the arrow going in the same direction.
Spending hours in boring college lectures with professors that lack the ability to teach and not learning ANYTHİNG vs watching a 6 minute video from a great teacher and understanding everything. I think I have spoken enough
All the videos I have viewed on DNA replication struggle to explain the 5'-3' process. Unfortunately, this wasn't any better, but the rest of the video was helpful.
Thank you Prof. Dave! I spent like two hours trying to understand my professors lecture, and you helped quite a lot! I am really grateful that I found this video online. Thank you for what you do! :)
DNA chain can only be synthesized in 5'-to-3' direction, not 3 to 5. As the subunits is being added at the 3' end, that means it runs in 5'-to-3' direction....
Actually he's correct, the DNA polymerase III moves in a direction of 3'- 5' of the preexisting strand. However, the new strand that's being synthesized is 5'-'3. The video is crystal clear .
The video is correct, but NOT clear. This "Dave" person knows the stuff but doesn't know how to explain it to a naive mind. Someone listening to this for the first time can get confused for all his life ! REPLICATION HAPPENS FROM 5 to 3 END !
sir you are a really talented teacher and a speaker ...... a process which would take me hrs to understand was made easy by you and i got it in juat 6 mins ! thanks man
Video was crystal clear . I understood the whole topic In ONLY 6 minutes where I didn’t get one thing through my head in a 80 minute class . Thank you so much Professor Dave !!
I enjoy that I can learn the basics from your mini-lectures. Great job, Professor Dave. You put me in mind of my late father, a physician, teaching me about reproduction, mitosis, meiosis, etc. He started with onions and went on from there, ending with human reproduction when he felt it was age-appropriate (about 13-14 in my case). This puts me in mind of his teaching methods. I just subscribed a couple of hours ago and will be adding your videos to my Geology/Volcanology and Evolution and Extinctions playlists. So glad I found your channel!
hi prof, had a few doubts: 1. after binding the primer to the DNA strand, the primase leaves the spot, and DNA polymerase iii takes the same spot to start adding the free DNA nucleotides? 2. on which strand do the following take place first or do the following processes take place simultaneously (as there is one molecule of enzyme allocated for each strand?) on both the strands? a) exonuclease removing the RNA nucleotides/primers b) DNA polymerase I adding DNA nucleotides in place of the primers c) Primase adding primers d) polymerase III adding free DNA nucleotides
Dave, polymerase III reads from 3' to 5' but polymerases new strand from 5' to 3'? But you said polymerase copies 3' to 5'. Did you mean polymerase reads 3' to 5'?
Not sure if nitpicky or not but it could help to clarify that DNA polymerase III/I are those used in prokaryotes. I remember in high school still using the terms generically but I believe DNA pol-alpha/delta are the current standards for eukaryotes?
As was stated previously, the direction is NOT 3' -5'. According to Mc Graw-Hill 11th ed. Biology, "As with all known DNA polymerases, all three of these enzymes synthesize polynucleotide strands only in the 5'-3' direction and require a primer"
i meant that it moves along the template strand 3'-5', though you are correct the new strand is synthesized 5'-3', perhaps i should have been clearer in the animation.
Its so frustrating, I understand the protein syntecis, the DNAs structure, mitoses and meiose, transcription, translation etc. when I get it explained to me, but there is no way I can repeat it😂😭
Loving the vids, I'm revising what I learned in the balkan equivalent of advanced biology & chemistry highschool. Just one thing: the audio volume is a bit low everywhere except the intro. These are old af but if you plan to make more videos maybe amplify the volume a bit in post.
Do you have a video about endogenous retroviruses (ERVs)? Would like to get a better grasp about the subject. Thanks for these videos btw. Really helpful.
Polymerase starts traveling at the 3’ end of the existing strand(so 3’to5’). The new strand it adds would be in the opposite direction. And that is what is confusing you.
Thank you very much for this lecture! But isn't it, that the direction is from 5' to 3' and not the other way around? At least that is what Amboss states.
Does DNA polymerase III only work on leading strand ? Does DNA polymerase I also work on leading strand to replace RNA nucleotide into DNA nucleotide ?
Didn't understand one thing. Why does the lagging stand require a separate primer for each okazaki fragment, when the leading strand is happy with only one primer?
because on the leading strand replication goes in the direction of the moving replication fork, on the lagging it goes the other way, so it has to synthesize a bit going the opposite direction, then some more when it becomes available, etc. it's all in here!
broo u make me think of my science teacher, I love listening to him, he has nice looking long hair which is cared and u understand every single word wtf this is freaky 😭🤚
So I am bit confused is it dna Polymerase 1 only need to go through lagging strand because the lagging strand have much more primer than the leading strands do?
Dear Prof nice to follow you. you have mentioned tow polymerase : polymerase I and III, what about polymerase II? is there II or not, if isn't, why number II has skipped?
![Felix "Unfair" | [Stray Kids : SKZ-PLAYER]](http://i.ytimg.com/vi/Oswujxm2Ag0/mqdefault.jpg)
Gotta love how with quarantined schooling teachers have given up on teaching and recognized that people like you have been the ones actually teaching us.
that why im currently watching this
@@Emily-gy8pe same!
🙌🏽
Professors and teachers are getting lazy and it is so much easier for them to just send us to a website and let someone else explain it! Thank you!
Even with a biology degree and in medical school, this is the most straight forward and easy to understand video on DNA replication. You sir are the GOAT.
You literally just summarized about half of what I learned in an entire semester-long molecular biology class in a 6 minute video. Nice.
Dang what!?!?!?!
really this is day one for us..... it is fricking hard XD
I love that I can learn by watching videos. I am interested in many subjects and my idea of a good session is learning something new. I will definitely be checking out Professor Dave!
Every time I struggle to understand a course material and find out you have a video for it, I cry tears of joy
I just realized something very important in terms of how to interpret your animation.
The directionality of the synthesis of the new strand is correct. However, I initially thought that the numbers you put on the leading strand was identifying the ends of the new strand, not the original strand.
In other words, the new strand would read/synthesize as such: 3' under the 5' of the leading strand and 5' under the 3' of the leading strand with the arrow going in the same direction.
You single handedly made me not only pass but EXCEL in my biology 101 class. Thank you professor dave
Spending hours in boring college lectures with professors that lack the ability to teach and not learning ANYTHİNG vs watching a 6 minute video from a great teacher and understanding everything. I think I have spoken enough
5' and 3' DNA template strand should be more specific indicated in the slide. Its a little bit confusing.
yeah a lot of people have mentioned this, i wish i had made it a bit more clear. nothing i can do now!
Yes, he needs to label the new and old strand as it is very confusing (he's labeling the old strand)......this is where students get mixed up often.
@@ProfessorDaveExplains
You should make another video...
Please sir this is a humble request...
All the videos I have viewed on DNA replication struggle to explain the 5'-3' process. Unfortunately, this wasn't any better, but the rest of the video was helpful.
@@ProfessorDaveExplains annotations?
Thank you Prof. Dave! I spent like two hours trying to understand my professors lecture, and you helped quite a lot! I am really grateful that I found this video online. Thank you for what you do! :)
DNA chain can only be synthesized in 5'-to-3' direction, not 3 to 5. As the subunits is being added at the 3' end, that means it runs in 5'-to-3' direction....
Bawan Colnadar I noticed that error in the vid as well
He's going to get people confused. Need an upgrade.
Actually he's correct, the DNA polymerase III moves in a direction of 3'- 5' of the preexisting strand. However, the new strand that's being synthesized is 5'-'3. The video is crystal clear .
The video is correct, but NOT clear. This "Dave" person knows the stuff but doesn't know how to explain it to a naive mind. Someone listening to this for the first time can get confused for all his life !
REPLICATION HAPPENS FROM 5 to 3 END !
Sounds like you're not interested in using your logic only in memorizing facts.
Bro, why does the microscopic world low key feels like a mini-society
You remind me of an off-brand Dave Grohl and it makes studying way easier, thank you
my test is in an hour and your videos are the only thing that has made me understand replication/transcription/translation omg 😭😭😭
sir you are a really talented teacher and a speaker ...... a process which would take me hrs to understand was made easy by you and i got it in juat 6 mins ! thanks man
That Addicted Guy you forgot the 15 seconds
Video was crystal clear . I understood the whole topic In ONLY 6 minutes where I didn’t get one thing through my head in a 80 minute class . Thank you so much Professor Dave !!
I like the fact that what I learned here was better than what I learned in school, more detailed and compact
Keep it up 😊
I enjoy that I can learn the basics from your mini-lectures. Great job, Professor Dave. You put me in mind of my late father, a physician, teaching me about reproduction, mitosis, meiosis, etc. He started with onions and went on from there, ending with human reproduction when he felt it was age-appropriate (about 13-14 in my case). This puts me in mind of his teaching methods.
I just subscribed a couple of hours ago and will be adding your videos to my Geology/Volcanology and Evolution and Extinctions playlists. So glad I found your channel!
A great, fast, easy and organized video! Thanks!
i have a bio final tomorrow i love u professor dave ure the goat
hi professor. i thought polymerase creates the leading strand in a 5'-3' direction towards the fork?
I love the way you explain each step of DNA replication and other biological processes in your videos, I'm just glad to find your channel :')
This man has Taught me for 3 separate courses, its amazing
If only I had you as my lecturer ! Absolute legend mate!
Thank you sir
I spend 2 days on this topic but this 6 minutes video clear my concepts too much
Sir, can you state the difference between DNA polymerase alpha beta, gamma, delta,epsilon and DNA polymerase 1,2,3??
What software was used to image the DNA molecule in this video (at about time stamp 00:07 - 00:15)?
oh that's just a png i found on google images, i'm not sure what it was made with
Makes so much more sense hearing it from you vs. in class
Whenever I am in need of help in biology, I go to this dude! Nice job! 👍🏻
Thanks for hearting!
hi prof, had a few doubts:
1. after binding the primer to the DNA strand, the primase leaves the spot, and DNA polymerase iii takes the same spot to start adding the free DNA nucleotides?
2. on which strand do the following take place first or do the following processes take place simultaneously (as there is one molecule of enzyme allocated for each strand?) on both the strands?
a) exonuclease removing the RNA nucleotides/primers
b) DNA polymerase I adding DNA nucleotides in place of the primers
c) Primase adding primers
d) polymerase III adding free DNA nucleotides
This is one of the best videos I have seen on DNA Replication!! thanks a whole whole lot!!
Its perfect!!!!😍
Each time i want to search for something scientific i find a video for you, your explanation is perfecttttt, keep on Dr.♥️
This is the only video I've seen that accurately explains the difference in role of Topoisomerase and Helicase...Thank you
I have a final task in Genetics, thanks for this vid. It means a lot to me!
I can leisurely watch Professor Dave in my free time and enjoy every second learning as many videos as I can.
This helped me so so much! Your video really cleared this up! Thank you!
Dave, polymerase III reads from 3' to 5' but polymerases new strand from 5' to 3'? But you said polymerase copies 3' to 5'. Did you mean polymerase reads 3' to 5'?
I said moves along the template strand, so yes "reads" would be a good way to interpret that.
I'm in 12 standard from INDIA 🇮🇳 & I found this video which really boosted my knowledge ☺
Not sure if nitpicky or not but it could help to clarify that DNA polymerase III/I are those used in prokaryotes. I remember in high school still using the terms generically but I believe DNA pol-alpha/delta are the current standards for eukaryotes?
As was stated previously, the direction is NOT 3' -5'. According to Mc Graw-Hill 11th ed. Biology, "As with all known DNA polymerases, all three of these enzymes synthesize polynucleotide strands only in the 5'-3' direction and require a primer"
i meant that it moves along the template strand 3'-5', though you are correct the new strand is synthesized 5'-3', perhaps i should have been clearer in the animation.
Thank you for your timely reply! Perhaps it could have been clearer, but you are right as well. Thanks for the video, anyhow :D
It is very impressive to listen your explanation specifically biology 🎉🎉🧬🧬🧬
Its so frustrating, I understand the protein syntecis, the DNAs structure, mitoses and meiose, transcription, translation etc. when I get it explained to me, but there is no way I can repeat it😂😭
Loving the vids, I'm revising what I learned in the balkan equivalent of advanced biology & chemistry highschool.
Just one thing: the audio volume is a bit low everywhere except the intro. These are old af but if you plan to make more videos maybe amplify the volume a bit in post.
This is such a great video! short and sweet :)
You are awesome.. I was jumping from video to another and I can’t understand that. But with you just in 6 min I take it very well !!
Your videos are so helpful!
Do you have a video about endogenous retroviruses (ERVs)? Would like to get a better grasp about the subject.
Thanks for these videos btw. Really helpful.
it was beautiful sir. it helped me memorize it smoothly.
I like how you dont use gimics at all, you tell what needs to be told and have easy to understand diagrams
Super helpful! Would have taken hours for me to understand just by reading this.
Thank you Sir!
Thank you couldn’t understand from the biology book with the diagrams you just made me understand in 6 minutes
holy shit i've been looking for videos like this all year
search no longer!
Explained in very easy and simple manner... Thank u sir
Thank you so much Prof Dave
I wish you could explain the rolling cycle mechanism too
Your explanation is very clear and the footnotes are very helpful, i now can understand the replication process thankyou prof
Best explanation I've ever heard and seen my whole life. Ily.
Great video... But did you forget SSBPs??
Watched so many videos on this and this one finally made sense. Thank you!
You have the replication going from 3' --> 5'. This is incorrect. Replication always goes from 5' -->3'.
the daughter strand is 5' to 3'. polymerase moves along the template strand 3' to 5'.
Yes Please See again...
Thank you so much! You helped clarify a lot of areas that my textbook failed to properly explain.
thank you so much, i am so grateful for this video. Explanations are perfect.
Thank you for saving my exam! Very clear and well structured explanation.
Thank you so much sir.... It really helped✌️👌
From Professor to Professor 👉🏼 you are great !!!
Doesn't polymerase travel 5' to 3' not 3' to 5'?
Polymerase starts traveling at the 3’ end of the existing strand(so 3’to5’). The new strand it adds would be in the opposite direction. And that is what is confusing you.
I'm confused ! isn't it always 5' to 3' direction ? In some videos I heard that DNA polymerase can only move in the (5' → 3') .
direction of motion along template and direction of synthesis are complementary
Thank you for this.. I had no idea what was going on until I saw your videos! Thank you so so much!
Thanks for this explanation. Some things are simplified. But this is a very good overview. It was really helpful.
And BOOM!!!! That's how I understood DNA replication!!!
one moment of silence for appreciating the complex administration of life
Great explanation, thanks!
Thank you very much for this lecture! But isn't it, that the direction is from 5' to 3' and not the other way around? At least that is what Amboss states.
Watch that section again and listen carefully.
Thank proff Devi .....am really enjoy your session
What happens to that mutation cells which polymerase and enzymes dont fix. Does that cells turn in cancer cells if immune system dont kill them?
Depends on the mutation. If it's in a proto-oncogene, then maybe.
Easy & concept clear explanation.... thank you sir.
you're the real MVP! and I love your intro lol it's hella catchy and cute
Does DNA polymerase III only work on leading strand ? Does DNA polymerase I also work on leading strand to replace RNA nucleotide into DNA nucleotide ?
Are DNA polymerases I and III in prokaryotic cells? What are the DNA polymerases in our cells(eukaryotic)?
You’re a life saver Professor Dave..
Thank you! Very helpful! Which okazaki fragment will be placed first in the lagging strand?
Hey Mrs.Hartley, am I still counted absent if I watch this full video?
I got more than I asked for! A lot of these stuff aren’t part of my textbook but I don’t care. I’ve always wanted to learn this anyway.
Didn't understand one thing.
Why does the lagging stand require a separate primer for each okazaki fragment, when the leading strand is happy with only one primer?
because on the leading strand replication goes in the direction of the moving replication fork, on the lagging it goes the other way, so it has to synthesize a bit going the opposite direction, then some more when it becomes available, etc. it's all in here!
Weird.
But, okay.
broo u make me think of my science teacher, I love listening to him, he has nice looking long hair which is cared and u understand every single word wtf this is freaky 😭🤚
Excellent video, Thanks!
Nice video but i've a little question, in the lagging strand .. why can't polymerase make the new strand with only one primer...?
because more template keeps becoming available as the helix unwinds! it needs a new primer for each new section.
Professor Dave Explains thanks
I love u Prof. Dave , u made it simpler
thank you so much for your explaining
Professor why the one strand is continuous and the other one is discontinue??
If there's DNA Polymerase I and III, then is there a DNA Polymerase II? If so, what does it do?
Can you explain the difference between DNA pol 1 and pol 3 a little more? I'm not getting it
Thanks for the help. Now I get DNA replication better than ever!
Which DNA polymerase are you referring to when you say DNA polymerase proof reads. Polymerase 1 or 3?
i believe all polymerases have proofreading ability
i believe i can fly
You just made this shenegan simple.
So I am bit confused is it dna Polymerase 1 only need to go through lagging strand because the lagging strand have much more primer than the leading strands do?
best DNA replication video!
Why RNA primer is used in DNA replication sir??plz reply
thank you so much for simplifying this for me. also, you kind of look like dave grohl? ;)))
Dear Prof
nice to follow you.
you have mentioned tow polymerase : polymerase I and III, what about polymerase II? is there II or not, if isn't, why number II has skipped?
Polymerase 2 is involved in DNA repiar not in DNA replication.
Medical Doctor thanks!
Wow! I love the lecture. Thank you Prof
Very detailed. Thank you very much! ♥️