Golden Gate Cloning

Heya there!
This is a video explaining a bit about Golden Gate Cloning, an elegant and simple technique to clone multiple fragments at once, using just one restriction enzyme. The so called "One step one pot" reaction.
I use this technique (or exactly a slight modification of it) in my usual cloning reactions.
I've wrote a tiny article on Biorxiv (preprint) in which I describe the method in detail (with protocols).
www.biorxiv.or...
This is the protocol I use for Site directed mutagenesis, called S.P.R.I.P :
bmcbiotechnol....
This is the original Golden Gate Assembly article :
journals.plos....
I also have videos on how to :
Do a restriction digest, do an electrophoresis, do a bacterial transformation etc.

[@nickst2797]

Thank you for all your work!

[@CanYouBio]

Thank you so much for commenting! If you have feedback or questions please feel free! :)

[@nickst2797]

@@CanYouBio Can you do more videos on how to do Gene Editing with software?

[@CanYouBio]

​@@nickst2797 I was thinking of making for example a CRISPR plasmid. Explaining how to use the Golden Gate Assembly tool from NEB so you can design your own primers for the assembly. Or using my own system. Or something simpler. Maybe an orange gene in bacteria :)

[@nickst2797]

@@CanYouBio These are all great ideas! Kinda like what The Thought Emporium used to do in the first lockdown. But instead of livestreams, guides. Thank you very much!

[@hamidkiangaikani]

That's a fantastic tutorial Adrian! Many thanks for that. Are you on Twitter? (:

[@CanYouBio]

No, sorry! Thank you so much for your feedback, though! I'm on facebook! Adrian Ionut Pascu. Keep in mind. This is not a "HOW TO" video, this is a HOW I DO video. My method is clearly not the best one but if it works for me I find it ok. Usually one uses blue white screening in golden gate standards.