A Detailed Look at Golden Gate Assembly

Delve deeper into Golden Gate Assembly with this detailed look. This video covers the features of Type IIS enzymes, Golden Gate destination vectors, preparing DNA fragments and the #GoldenGateAssembly reaction.
IN THIS VIDEO:
00:00
00:14 Type IIs Enzymes
02:04 Destination Vectors
02:46 Preparing DNA Fragments
03:49 Golden Gate Reaction
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[@llllllllllllllllllllllllIIIIl1]

Bsa1 cleaves one away from its restriction sites for top strand and five away from the bottom strand. it still gives 4 overhangs but the graphic at 0:49 are slightly inaccurate

[@kiranysn.6484]

how is it not paslindromic?in the video the sequence is palindromic
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[@nya4442]

tbh i have the same question 😂

[@rezanadaf1213]

It is not palindromic you fool that would mean it is read same forward as read backward...is GAATTC the same as CTTAAG? NO! Get GUD son! TATAT is an example of a PALINDROME FOOL! Reversed it is still TATAT

[@remcomentink6842]

What is shown is actually an EcoRI site, no idea why :p

[@bofriedbo]

No? It is not palindromic in the video, i'm wondering how you thought it was 🤔

[@DaveSmith-mv8ex]

The sense sequence read backwards is the same as the antisense

[@svenvida9590]

Really helpful Video, thanks

[@SnapGene]

Glad it was helpful!