A Detailed Look at Golden Gate Assembly

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  • Опубликовано: 5 янв 2025

Комментарии • 10

  • @llllllllllllllllllllllllIIIIl1
    @llllllllllllllllllllllllIIIIl1 Год назад +3

    Bsa1 cleaves one away from its restriction sites for top strand and five away from the bottom strand. it still gives 4 overhangs but the graphic at 0:49 are slightly inaccurate

  • @kiranysn.6484
    @kiranysn.6484 2 года назад +20

    how is it not paslindromic?in the video the sequence is palindromic
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    • @nya4442
      @nya4442 2 года назад +3

      tbh i have the same question 😂

    • @rezanadaf1213
      @rezanadaf1213 Год назад

      It is not palindromic you fool that would mean it is read same forward as read backward...is GAATTC the same as CTTAAG? NO! Get GUD son! TATAT is an example of a PALINDROME FOOL! Reversed it is still TATAT

    • @remcomentink6842
      @remcomentink6842 Год назад

      What is shown is actually an EcoRI site, no idea why :p

    • @bofriedbo
      @bofriedbo 3 месяца назад +1

      No? It is not palindromic in the video, i'm wondering how you thought it was 🤔

    • @DaveSmith-mv8ex
      @DaveSmith-mv8ex 2 месяца назад

      The sense sequence read backwards is the same as the antisense

  • @svenvida9590
    @svenvida9590 2 года назад +2

    Really helpful Video, thanks

    • @SnapGene
      @SnapGene  2 года назад

      Glad it was helpful!

  • @Andres-pe3eq
    @Andres-pe3eq 5 дней назад

    this is really hard to understand