Hi i would like to set up my own lab to analyze heroin samples, is there any way you could give me a list of equipment needed? Would be great if you could, I am looking for the cheaper stuff as I will be paying out my own pocket. I believe every town should have this kit with fentanyl around.
To set the scale. Geometry, two points define a line, and if you know the exact distance between the two known points, that sets the scale of the line.
Actually the best way is a lot easier. You have them made by accountable people in proper pharmaceutical production facilities. If we did that, here would be no need to test pills at all. This problem only exists because of prohibition. If GDS is really concerned about the contamination problem, why not highlight the solution?
Yes and yes Pma feels horrible but slightly trippy not like md and it takes a long time to kick in so if you don’t feel anything after the usual mdma come up time don’t take another because it may cause death
It wasn't exactly clear but it appears that he has a large bottle of methanol that he uses for the extraction, and then a smaller bottle of methanol spiked with a known quantity of an internal standard--the identity of this compound is unrelated to what you're analyzing. Instead, when you know there should be 1000 units of internal standard and you only measure 900 units on the GCMS you know that you lost 10% of your sample during the analysis and that loss is correlated to the compound you actually care to measure. (for anyone interested you could also include a spike of standard in the extraction/centrifugation (sample prep) process to measure for any loss during sample prep. Instead of being called internal standard this would be called a surrogate standard.)
With the second solvent, he diluted the 10uL of sample (which is likely quite concentrated) to a total volume of 1.010mL to avoid injecting too much sample into the instrument (this is an instrument--not a machine). The GC is composed of a small diameter column (think tube), typically 0.25mm inner diameter, and cannot handle too much sample, and the MS has the ability to "see" very small quantities of sample--50nanograms of each molecule is more than enough to "see". A typical injection volume is only 1uL (1/1000 of a milliliter) at a concentration of ~50ppm and will give a very strong signal in a properly operating instrument. So in short, the second solvent is simply there to dilute the sample down to an acceptable concentration for this highly sensitive instrument. It should be noted too that with GC, you often have to chemically modify (derivatize) the samples so they can exist in the gas phase at reasonable temperatures. A typical GC column can only be heated to 340*C, so if the sample has a boiling point near or above that, it won't travel through the column, and therefore never be "seen" by the MS. This is the case for polar molecules that have substantial intermolecular forces (remember London dispersion forces, hydrogen bonding, & dipole-dipole interactions from high school chem class? they lead to high boiling points), but not so for molecules that have limited intermolecular forces (and subsequently lower boiling points).
Mitchell Helling Hey, in the video he does actually say that the 2nd bottle of methanol contains internal standard, it wasn't just for dilution. The rest of what you were saying I'd agree with though :). (and what you're most correct about is that he has to bring the concentration of the unknown analytes down to an acceptable range so he doesn't crap up his inlet or dirty the source. Along with the chomatographic issues you have if you overload the capacity of the column.)
My guess was MDMA or MDA and/or some other type of rave stimulants. Close enough for government work, and my analysis didn’t require a $100,000+ machine!
Chemistry technology due to its expense tends to lag behind somewhat. My school's apparatus require that you have a floppy disk in the actual machine, after which you move it on to the network or a flash drive.
ethanpet113 well actually some of the machines are only compatible with older versions of windows, anyways they’re only for collecting data which doesn’t require much computing power
Since most of our pharmaceuticals are made in India and China under very questionable quality control, it would be prudent to have them analyzed from time to time. God forbid the FDA would recognize the logic of this and utilize tax payer money to protect the public.
Do you offer your sevices to public, if so how much will it cost? If not, who will?
Thanks for a really simple & interesting explanation on GCMS :)
Where can one go to have a pill tested?
Any police station will help. Just see the sergeant and tell him what you have
Good morning. Do we use the same procedure to prepare crystal methamphetamine for it to be analysed with GC-MS
Yes, any common organic solvents like methanol, ethanol, and acn are fine.
Hi i would like to set up my own lab to analyze heroin samples, is there any way you could give me a list of equipment needed? Would be great if you could, I am looking for the cheaper stuff as I will be paying out my own pocket. I believe every town should have this kit with fentanyl around.
Same!
Very good tutorial/informative video! Howcome you use two internal standards and not just one? Thank you! :)
To set the scale. Geometry, two points define a line, and if you know the exact distance between the two known points, that sets the scale of the line.
What is the make and model of the gscm machine ?
this excites the drug nerd in me
What is the rough cost of a GCMS survey for a tablet like this?
So how much for you to test micro samples of my stash?
Please can tell me what is the internal standard
Just posted on theobromine...well Caffeine...lol.
But will not work for the analyt due to subject sensitive knows when compounds flux, "knew" when 1 compound substitute for the other!🥶😉😶👁🤭👌
Actually the best way is a lot easier. You have them made by accountable people in proper pharmaceutical production facilities. If we did that, here would be no need to test pills at all.
This problem only exists because of prohibition. If GDS is really concerned about the contamination problem, why not highlight the solution?
PMA, isn't it rare in ecstasy tablets? And can i feel the PMA if It's in my ecstasy pill?
Yes and yes
Pma feels horrible but slightly trippy not like md and it takes a long time to kick in so if you don’t feel anything after the usual mdma come up time don’t take another because it may cause death
What is the filler attached to the methanol bottle called please? Need to buy a similar item.
You probably got the answer by now, but if you haven't a simple google search of "laboratory dispenser" will show you.
bottle-top dispenser, it is an adjustable pipette.
bottle-top dispenser, it is an adjustable pipette.
PMA, Caffeine... "he said green tablet? Lmao."
the extracting solvent is methanol....what is that second solvent you added that contains what standard? i didnt get that part...thank you
It wasn't exactly clear but it appears that he has a large bottle of methanol that he uses for the extraction, and then a smaller bottle of methanol spiked with a known quantity of an internal standard--the identity of this compound is unrelated to what you're analyzing. Instead, when you know there should be 1000 units of internal standard and you only measure 900 units on the GCMS you know that you lost 10% of your sample during the analysis and that loss is correlated to the compound you actually care to measure.
(for anyone interested you could also include a spike of standard in the extraction/centrifugation (sample prep) process to measure for any loss during sample prep. Instead of being called internal standard this would be called a surrogate standard.)
With the second solvent, he diluted the 10uL of sample (which is likely quite concentrated) to a total volume of 1.010mL to avoid injecting too much sample into the instrument (this is an instrument--not a machine). The GC is composed of a small diameter column (think tube), typically 0.25mm inner diameter, and cannot handle too much sample, and the MS has the ability to "see" very small quantities of sample--50nanograms of each molecule is more than enough to "see". A typical injection volume is only 1uL (1/1000 of a milliliter) at a concentration of ~50ppm and will give a very strong signal in a properly operating instrument. So in short, the second solvent is simply there to dilute the sample down to an acceptable concentration for this highly sensitive instrument. It should be noted too that with GC, you often have to chemically modify (derivatize) the samples so they can exist in the gas phase at reasonable temperatures. A typical GC column can only be heated to 340*C, so if the sample has a boiling point near or above that, it won't travel through the column, and therefore never be "seen" by the MS. This is the case for polar molecules that have substantial intermolecular forces (remember London dispersion forces, hydrogen bonding, & dipole-dipole interactions from high school chem class? they lead to high boiling points), but not so for molecules that have limited intermolecular forces (and subsequently lower boiling points).
Mitchell Helling Hey, in the video he does actually say that the 2nd bottle of methanol contains internal standard, it wasn't just for dilution. The rest of what you were saying I'd agree with though :). (and what you're most correct about is that he has to bring the concentration of the unknown analytes down to an acceptable range so he doesn't crap up his inlet or dirty the source. Along with the chomatographic issues you have if you overload the capacity of the column.)
hexane or isooctane
I love the speed of the injector
Nearly three years later, how do I befriend this guy? No reason.
very informative video ..
Hi sir , iam using this technique a reference for it please please .
How I get job in this field any reference
A MSc or above in chemistry I’m guessing.
you saved someon's live doc, PMA is bad shit no cap
My guess was MDMA or MDA and/or some other type of rave stimulants. Close enough for government work, and my analysis didn’t require a $100,000+ machine!
With this test can we know the ingredients of the pill and how many milligrams are in the pill?
The two wackest things that you DONT want in your pills. This must be from around 2008 all the pills I see now days are MDMA again fortunately
Is he running windows '98?
Chemistry technology due to its expense tends to lag behind somewhat. My school's apparatus require that you have a floppy disk in the actual machine, after which you move it on to the network or a flash drive.
ethanpet113 well actually some of the machines are only compatible with older versions of windows, anyways they’re only for collecting data which doesn’t require much computing power
Cool! Thanks!
Thanks sir
3:39 "ah bloody ell"
PMA and caffine, thats a death tablet there
Hey can you test yellow snapchats and red supreme please that would be a nice video
Do they REALLY call them such shitty names nowdays? Or you just havin a laugh? Whatever happened to good ol Mitsus and Smileys? 🤣🤣🤣
How can I do this at home?
PMA wow dangerous, surprised that's in any tablets anymore..
Don't we need to prepare dilutions
Since most of our pharmaceuticals are made in India and China under very questionable quality control, it would be prudent to have them analyzed from time to time. God forbid the FDA would recognize the logic of this and utilize tax payer money to protect the public.
Lol. Myself included, I have another way, much shaking.
damn that was fake MDMA