OpenFlow: Introduction to Panel Design

We have shown you how to set up a flow cytometer, looked at compensation and data analysis and now is the time to look at panel
design! In this session we will look at what makes a good fluorochrome choice, how we need to consider sample preparation and fixation
and how we can begin to think about making an optimized combination for your cytometer. With a mixture of theory and practice, this
session is suitable for those beginning in flow cytometry and is a place to hear about current best practices.

[@Science.Medicine209]

When Kathy explains, every topic in flow; flows like a flow

[@Science.Medicine209]

I don't understand why Rui Gardner got issues every signal time"...his issues are more complex than Flow...😉😉

[@fabiankeppler315]

Why would there be a difference between DIVA Experiment FCS files vs Exported FCS files? It's raw data, is it not?
I also do not understand why the sorter would sort less good, having a double positive cell population.
If purity was the goal, wouldn't we expect a 'clean' sort result, having the gates set correctly and sort set to purity.
Efficiency will be low, but why a low purity?

[@DerekDaviesCytometry]

Hi Fabian, Diva produces .fcs files but they are stored in the database with numerical file names - to preserve the naming that was performed in Diva they need to be exported via the export function. It is also possible to export the experiment which would contain plots and gating etc. Sorting question: Doublet discrimination is never perfect and a doublet of a positive and negative cell could be sorted as a positive and this will impact on purity. This would not be the case if we sorted two positives or two negatives together.