Видео 19
Просмотров 133 414

OpenFlow: Multicolor Flow With Compensation Using a CytoFLEX LX

1:37:40

Antibody Titration Using a CytoFLEX LX

1:31:50

OpenFlow: Cell Proliferation by Flow Cytometry II

1:31:46

OpenFlow: DNA Analysis by Flow Cytometry

1:34:12

OpenFlow: Introduction to Panel Design II

1:38:49

OpenFlow: Introduction to Panel Design

1:30:17

OpenFlow: Three Colour Immunophenotyping and cell cycle analysis

In this session we will look at the cell cycle profiles of phenotypically defined subsets using a full spectrum cytometer. This will pull
together some of the things we have looked at in previous sessions - the importance of sample preparation, consideration of
fluorochromes, and controls. We will look at the set-up of the cytometer and the plots and gates needed to produce optimal results. We
will be using human blood cells and a Cytek Aurora.

Видео

OpenFlow: Multicolor Flow With Compensation Using a CytoFLEX LX

1:37:40

OpenFlow: Multicolor Flow With Compensation Using a CytoFLEX LX

Просмотров 5 тыс.Год назад

In any multicolor experiment, we will need to account for spectral spillover using the process of fluorescence compensation. In cytometers that allow users to change voltages or gains, adjusting the fluorescent detector settings is a critical step as this will affect the compensation values. In this webinar we will use the CytoFLEX LX flow cytometer and Slingshot Bio's TruCytes™ TBNK Controls &...

1:31:50

Antibody Titration Using a CytoFLEX LX

Просмотров 4,2 тыс.2 года назад

In this webinar we will re-visit one of the important parts of setting up a successful flow cytometry experiment - titration of antibodies which will allow us to determine the optimal concentration to use in a flow cytometry experiment. This time we will be using a Beckman Coulter CytoFLEX to run and analyse samples. We will cover some tips and tricks as well as highlighting best practices. As ...

OpenFlow: Cell Proliferation by Flow Cytometry II

1:31:46

OpenFlow: Cell Proliferation by Flow Cytometry II

Просмотров 3,2 тыс.2 года назад

In this session, we will continue to look at the ways cells divide but using more complex approaches - BrdU uptake and dye dilution. We will look at the theory behind these techniques, run samples on the cytometer and analyse the data produced to illustrate best practice in all these areas.

OpenFlow: DNA Analysis by Flow Cytometry

1:34:12

OpenFlow: DNA Analysis by Flow Cytometry

Просмотров 1,9 тыс.2 года назад

In this session we will look at how flow cytometry can be used to measure DNA content in mammalian cells. We will look at the available DNA dyes, the importance of sample preparation, cytometer set-up and how to perform the correct analysis. The session will be a mixture of theory and practical and we will be returning to the cytometer to give tips and tricks in machine set-up, pitfalls to watc...

OpenFlow: Introduction to Panel Design II

1:38:49

OpenFlow: Introduction to Panel Design II

Просмотров 1,2 тыс.2 года назад

We have shown you how to set up a flow cytometer, looked at compensation and data analysis and now is the time to look at panel design! In this session we will look at what makes a good fluorochrome choice, how we need to consider sample preparation and fixation and how we can begin to think about making an optimized combination for your cytometer. With a mixture of theory and practice, this se...

1:30:17

OpenFlow: Introduction to Panel Design

Просмотров 2,5 тыс.2 года назад

OpenFlow: Introduction to Flow Cytometric Data Analysis Part III

1:27:15

OpenFlow: Introduction to Flow Cytometric Data Analysis Part III

Просмотров 3,3 тыс.3 года назад

In this third session we will look in more detail at offline compensation using both FlowJo and FCSExpress to show manual and automatic compensation and how to assess success. We will run this analysis live and interactively allowing delegates to ask questions as we go along.

OpenFlow: Introduction to Flow Cytometric Data Analysis Part II

1:39:22

OpenFlow: Introduction to Flow Cytometric Data Analysis Part II

Просмотров 6 тыс.3 года назад

In this second session we will look in more detail at how we can report metrics to answer biological questions which will include looking at statistics, the proper use of controls and how to display your data. In this session we will use FCSExpress software. We will run this analysis live and interactively allowing delegates to ask questions as we go along.

OpenFlow: Introduction to Flow Cytometric Data Analysis Part I

1:33:26

OpenFlow: Introduction to Flow Cytometric Data Analysis Part I

Просмотров 39 тыс.3 года назад

In this session, we looked at data analysis of flow cytometry files. In this first session presented an overview of data analysis and how it can be used to answer the biological questions you have using FlowJo software. This included quality control, choice of plots, regions and gating, data scaling and selection of appropriate metrics.

OpenFlow: Introduction to Cell Sorting Part III

1:39:17

OpenFlow: Introduction to Cell Sorting Part III

Просмотров 1,9 тыс.3 года назад

The success of a cell sort depends on many factors including the sample preparation and knowledge of how the sorter works. In this session, we will look at what can go wrong and see the importance of experimental optimization. Delegates will also discover how to troubleshoot the most common problems and will have the opportunity to ask questions. Note: At the end of this session an issue was se...

OpenFlow: Introduction to Cell Sorting Part II

1:47:54

OpenFlow: Introduction to Cell Sorting Part II

Просмотров 5 тыс.3 года назад

In this OpenFlow session we will review the theory and practice of cell sorting. We will consolidate knowledge from our previous session and use a BD FACSAria to perform a more complex cell sort. The aim of this session is to show attendees the theory of cell sorting in addition to the practicalities of performing a successful sort.

OpenFlow: Introduction to Cell Sorting Part I

1:38:05

OpenFlow: Introduction to Cell Sorting Part I

Просмотров 8 тыс.3 года назад

In this sessions, we will explore cell sorting by flow cytometry. This is an extremely useful technique as it allows us to separate cells of interest for further study. However, the success of a sort depends on many factors including the sample preparation and knowledge of how the sorter works. In part I, we will look at the theory of cells sorting and perform a simple sorting experiment using ...

OpenFlow: Full Spectrum Flow Cytometry Part II - Panel Design & Troubleshooting

1:49:40

OpenFlow: Full Spectrum Flow Cytometry Part II - Panel Design & Troubleshooting

Просмотров 3,1 тыс.3 года назад

In this session, we will continue to look at full spectrum flow cytometry. Specifically, we will review considerations for fluorescence panel design. What do we have to think about to design a successful panel and how can we combine fluorochromes that might be difficult on a conventional flow cytometer? We will also look at autofluorescence extraction and present some troubleshooting tips. The ...

OpenFlow: Full Spectrum Flow Cytometry with the Cytek Aurora

1:40:07

OpenFlow: Full Spectrum Flow Cytometry with the Cytek Aurora

Просмотров 12 тыс.3 года назад

In this OpenFlow session we will review the theory and practice of full spectrum flow cytometry. We will address some of the fundamentals behind full spectrum flow cytometry and how it compares with the current technology, as well as running a multiparameter experiment on a five-laser Cytek Aurora using Spectroflo software.