Microbiology Techniques: Making a streak plate

Vicki Symington (Microbiology Society) and John Schollar (National Centre for Biotechnology Education, Reading) demonstrate how to make a streak plate of culture. Streak plates can be used to check the purity of a culture, and for spreading out a culture.
The Microbiology Society is a membership charity for scientists interested in microbes, their effects and their practical uses. It is one of the largest microbiology societies in Europe with a worldwide membership based in universities, industry, hospitals, research institutes and schools.
Our principal goal is to develop, expand and strengthen the networks available to our members so that they can generate new knowledge about microbes and ensure that it is shared with other communities. The impacts from this will drive us towards a world in which the science of microbiology provides maximum benefit to society.
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[@PhuongNguyen-uk7xj]

Lab is the only place where I love to see ppl "cheat" on their techniques, some are just paranoid, but some are really creative and life saving.

[@ehsankhan9177]

A very clear description of plate streaking

[@heanna172]

Both of you explain it really clear! Good job :)

[@jojo-vm6dt]

Great demo

[@LONEWOLF_APOCALYPSE]

Pls add microbial techniques for fungal culture on pda

[@salahaldinmohamed7940]

good job
tq from Libya

[@pzszify]

Excellent demonstration of a streak plate. What is a diameter of a standard petri dish like the dish shown in the video? Thanks.

[@auntiecarol]

100mm

[@pzszify]

Thank you

[@ShopperPlug]

Its weird that they did not give her a microphone... couldn't hear what she was saying. You guys are making twitter hashtags lol What if you know for a fact there is 4 different microorganisms in one petri dish and you did the "streaking" on the plate and all the bacteria ended up being same color, as if the 4 different microorganisms share the same color. How to isolate the bacteria in this scenario?

[@raghadabdu2507]

culture technique differ from year to year !

[@ahamedimthikab5779]

Yes there is the traditional streaking method which is not as efficient as this

[@lostinthoughts13]

Why flame the loop when your are working on the same thing like let me just show you when, 6:00

[@audrastephenson8747]

lost in thoughts from what I can tell it’s because you’re trying to dilute the culture each time you move to a different direction. If you flame the loop, you get those nice incremental decreases like you see at 9:30 because you’re not taking all the stuff you picked up in the previous detection with you to the next streak.

[@lostinthoughts13]

@@audrastephenson8747 aha thanks a lot

[@audrastephenson8747]

lost in thoughts no problem! Glad to help!