Microbiology Techniques: Serial dilution

Vicki Symington (Society for General Microbiology) and John Schollar (National Centre for Biotechnology Education, Reading) demonstrate how to do a serial dilution.
The Microbiology Society is a membership charity for scientists interested in microbes, their effects and their practical uses. It is one of the largest microbiology societies in Europe with a worldwide membership based in universities, industry, hospitals, research institutes and schools.
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[@SamanehKeshavarz-k6b]

Thank a lot. However, Why is the second Bunsen burner on for no reason?

[@7906jun]

thanks. but shouldn't they keep the bunsen burner to pilot mode when not using it.

[@zalkhanabdullah5584]

Nice and so clear and well understandable method for lecture thanks you so much

[@hanoonayassin8697]

thanks ... great work

[@campbellm2530]

everybody's gangsta until he pulls out the frozen peas

[@AaroInTheKnee]

Lab safety!

[@darlenedeguzman4999]

(Time: 20:32) I'm sorry, just a quick q. where'd u guys get "50"?
Btw...awesome vid :))

[@jinty1232]

50 is the dilution factor. 20 ul out of 1 ml was spotted onto the plate. If you multiply 20 ul by 50 it equals 1 ml.
There were 18 colonies in 20 ul, therefore 18 x 50 = 900 colonies in 1 ml.

[@aliaajwa3996]

Really easy to understand! Thank you

[@jojo-vm6dt]

Good demo

[@adilarahimi3932]

great work, but I have one question, why was it x by 50? anyone plzz?

[@garytahmahkera9934]

20 ul is a 1/50 dilution of 1000 ul or 1mL.

[@danwittek985]

1000uL(1mL) divided by 20uL = 50; if 20uL = 18 colonies then 1000ul = 900 colonies. If you wanted to know how many colonies per 100uL you would multiply both sides by 5.

[@Raminder_Singh]

Gloves

[@mrpeanutbutter6094]

Not a great idea when working next to fire

[@clo1584]

you're working next to a blue flame bunsen... definitely not a good idea to wear gloves.

[@petermarwadelighton3544]

Thank you so much for your nice lectures... really they are helpful but one thing is the lady does not pronouns the words well to be clearly heard like the man....... and if possible she has to reduce the speed and clear spell the worlds well.... i need to use more energy listening to her... let her regulate that because u need to know that you are teaching the world, thank u we r together