Pathogenicity of Bovine Bacterial Plasmid Derived Virus Like Infections

Numerous global epidemiological studies have established a significant connection between the consumption of red meat and dairy products and the incidence of specific cancers and neurodegenerative diseases. The spatial distribution of these cases has suggested a distinct role related to the consumption of such products derived from Eurasian cattle.
In response, our research endeavor focused on exploring potential infectious agents present in the serum and dairy products of these cattle. Through these efforts, we successfully isolated and sequenced over 30 single-stranded circular DNAs. These DNA fragments varied in length from approximately 1,000 to 3,000 nucleotides.
Categorically, we identified four distinct families and designated them as bovine meat and milk factors (BMMF). While one subgroup showed differences, the remaining isolates demonstrated remarkable nucleic acid similarities to plasmids found in Acinetobacter and Psychrobacter bacteria.
Upon introducing these DNA fragments into human cells via transfection, we observed transcriptional and translational activity across all tested samples. In certain human cells, we noted instances of replication and synthesis leading to infectious progeny. Crucially, the ability of these DNA fragments to infect cells was contingent on specific sialic acid components, suggesting their role as integral parts of cellular receptors.
Interestingly, four of the isolated DNA fragments were extracted from the sera or post-mortem brain samples of patients diagnosed with multiple sclerosis (MS). Through serological analyses utilizing consensus protein epitopes of the BMMF-1 group, we found elevated antibody titers in MS patients compared to healthy controls. A model elucidating the pathogenesis of MS has already been published based on these findings.
Currently, we are investigating the seroreactivity of patients afflicted with malignant tumors and neurodegenerative diseases in relation to antigens from all four isolated BMMF groups.
The data we have amassed represents a groundbreaking instance of bacterial plasmid-derived sequences that have been adapted to express genes, replicate autonomously, and generate infectious offspring within human cells. This discovery paves the way for novel methodologies in exploring their potential involvement in diet-related cancers, neurodegenerative disorders, and autoimmune diseases.
DOI: doi.org/10.544...
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