How to Identify Bacteria by 16S rRNA | EzTaxon vs NCBI Database Comparison| Phylogenetic analysis
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- Опубликовано: 5 фев 2025
- 🧬 How to Identify Bacteria Correctly by 16S rRNA | EzTaxon vs NCBI Database Comparison
In this video, I’ll show you how to accurately identify bacteria using the 16S rRNA gene and compare two powerful tools: EzTaxon and NCBI. Learn about the strengths and limitations of each database, and find out which one is best for bacterial species identification in your research. Whether you're working in microbiology, bioinformatics, or just curious about 16S sequencing, this video has you covered!
🔍 Topics covered in this video:
What is the 16S rRNA gene and why is it important?
Overview of EzTaxon and NCBI databases
Differences in accuracy, curation, and ease of use
Tips for choosing the right database for your work
💡 Why this matters:
Accurate bacterial identification is key in microbial research. By the end of this video, you’ll know which tool to trust for your 16S sequencing data and how to improve the quality of your bacterial identifications!
📌 Useful Links:
EzBioCloud (EzTaxon): www.ezbiocloud...
NCBI BLAST: blast.ncbi.nlm...
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👍 Like this video if you found it helpful, and leave a comment below with your thoughts on EzTaxon vs. NCBI!
🧑🔬 Tags:
#16SrRNA #BacterialIdentification #Bioinformatics #Microbiology #EzTaxon #NCBI #16SSequencing #BacterialGenome #Genomics #DrSajjad #BacteriaIdentification #NCBIBlast #BioinformaticsTips #MolecularBiology
thank you for all these informative informations, when I blast my sequences in NCBI the first seq appear is not the same first seq appear using EZtaxon, however %similarity in EZtaxon indicate 99.40% and ncbi indicate 99.02% / why there is difference / why not appearing the same seq for both of database
The difference in results between EzBioCloud (EzTaxon) and the NCBI database is due to the type of sequences each provides. EzBioCloud only includes type strains, which are manually curated and accurate, while NCBI offers a broader range of sequences that are not manually curated. You can choose either database based on your study needs. If NCBI is yielding satisfactory results, you may prefer to use it.
@Dr.Sajjadasaf it is 16s RNA, what do you advise please to use
@ If you obtain similar results and they show the same species, it's better to use EZTaxon. However, if the results differ significantly in NCBI, it's preferable to use NCBI, as you’ll need to submit there to receive an accession number.
@@Dr.Sajjadasaf it is showing a different species with the same genus, but EZtaxon shows the highest similarity percentage
@ then use eztaxon