One way I can think of is to first spot check how many RNA-seq reads map to a select few genes in IGV. Then using GTF file to quantify those reads and checking whether we get close numbers of quantified reads to the same set of genes as observed in IGV. That could give us an idea on how correct are the annotations.
hii. thanks for making such beautiful videos. A little request. Can you please make a video on analysing rna-seq data on the usegalaxy web tool? thanks.
Hi thanks for your videos. Why do u not start a course with very basic coding detail . As I dont have experience in coding and find it difficult to understand the scripts language. Moreover I would appreciate your if u start a series of videos with aim.of teaching analysis of GWAS studies and applying statistical models on data.
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Starting with very basic concept and then taking it to the next level --- wow -- Beautifully explained.
Explained very clearly. Easy to understand
Your channel is the best!
U really explain very beautifully
Thank you!! At 7:06 where is the upper part of the slide taken from?
It is just a screenshot of DLK1 gene in IGV.
Since you are discussing file formats please discuss VCF and BED formats too. It would be a great help.
These are definitely in the list.
As always, syper helpful!
You the best! How can some use RNA seq data to validate if annotations are correct?
One way I can think of is to first spot check how many RNA-seq reads map to a select few genes in IGV. Then using GTF file to quantify those reads and checking whether we get close numbers of quantified reads to the same set of genes as observed in IGV. That could give us an idea on how correct are the annotations.
hii. thanks for making such beautiful videos. A little request. Can you please make a video on analysing rna-seq data on the usegalaxy web tool? thanks.
Hi . Can u make tutorials for Hi C and HiChIP data analysis
I will surely consider making a video covering these topics :)
Hi thanks for your videos.
Why do u not start a course with very basic coding detail . As I dont have experience in coding and find it difficult to understand the scripts language.
Moreover I would appreciate your if u start a series of videos with aim.of teaching analysis of GWAS studies and applying statistical models on data.
These are great suggestions, thank you. I will surely think about creating a series of videos covering basics of coding and performing GWAS.
Hello. Thank you very much for the video.
Can you make a video on GWAS.
Thank you
I will surely consider making a video covering GWAS. Thanks for the suggestion :)