I've been counting on your videos since the start of A-levels and now I'm doing bio at my first choice uni, still coming back to your videos since they are so clear and concise, I truly thank you so so much
4:58 this only gives one of the sequences though? what about the other ones written above? Also, how come the bases are shown as if they are in different wells if they are from the same sequence? I understand the process but I don't get how to intepret it
Thank you so much for this video :D I just had one question: If a question asked, whats the difference between PCR and This method, what would you have to write I am very sorry for the last minute question :(
Ahh sorry I might have missed it by now...! It's hard to say what exactly you'd have to write, since I don't know what the other method is. But I'd think you can compare the temperatures and enzymes used. Thanks for watching!
At 2:59 when you are discussing the lack of an oxygen atom where the hydroxyl group would be present on carbon 3 of the pentose sugar do you mean deoxyribose seeing as we are using DNA nucleotides in the synthesis of the new double-stranded DNA molecules, I was wondering because you said ribose.
Shouldn’t we only add free nucleotides that aren’t the same type as the terminator bases? For example, lets say in a test tube we add the terminator base A, should the “ free nucleotides” added only be T,C,G? Otherwise the chain wouldn’t always terminate at the base A ?
DNA profiling is separating DNA fragments based on their lengths, which is useful for identifying individuals based on their genetic profiles. You will see bands of DNA fragments with the help of gel electrophoresis. DNA sequencing is finding the order of nucleotide/bases that make up an individual's genome. The result is a sequence of A/T/C/G.
@@BioRach This is going to sound like such an obtuse question but why does it need to be separated and sequenced if it is already in the sequence ? i understand the process but for me to have the confidence to answer the more implicit and nuanced questions with answers you dont find in textbooks but rather initiative , i need to know why it has to be sequenced also thank you for these two clear definitions
@leen908 You might not need this anymore but in case it helps anyone ....we don't know what the sequence is until we analyse the fragment lengths by electrophoresis. each individual fragment tells us the DNA base present at "that length" of the sequence (because each fragment ends in the corresponding terminator base), and since we have the full range of sequence positions, we have the base present at every position. if we order them by size, we get the entire dna sequence.
It's not really specified in the spec, but I would assume so as DNA sequencing is done using a thermal cycler (same as in PCR) which changes its temperature frequently. Therefore it'll need the Taq polymerase to withstand the high temperature. Hope this helps :D Thanks for watching!
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I've been counting on your videos since the start of A-levels and now I'm doing bio at my first choice uni, still coming back to your videos since they are so clear and concise, I truly thank you so so much
So pleased for you! And I'm glad to help :)
This is the topic i struggle with most, thank you so much
Glad the video helped :) Thanks for watching!
These are so helpful! Thank you so much! These have helped so much with my revision so far!
And thank you for watching! I'm really glad to hear that you find them helpful :D
this is amazing, thank you!
Glad it helps :)
thank youuuu！plz upload more videos
4:58 this only gives one of the sequences though? what about the other ones written above? Also, how come the bases are shown as if they are in different wells if they are from the same sequence? I understand the process but I don't get how to intepret it
Thank you so much for this video :D
I just had one question:
If a question asked, whats the difference between PCR and This method, what would you have to write
I am very sorry for the last minute question :(
Ahh sorry I might have missed it by now...!
It's hard to say what exactly you'd have to write, since I don't know what the other method is. But I'd think you can compare the temperatures and enzymes used.
Thanks for watching!
Your work is so amazing. Thank you so so so much.
Please could you do hormonal communication too x
haha glad you find it helpful! Will add this onto the list :D Good luck with revision and thanks for watching! :)
At 2:59 when you are discussing the lack of an oxygen atom where the hydroxyl group would be present on carbon 3 of the pentose sugar do you mean deoxyribose seeing as we are using DNA nucleotides in the synthesis of the new double-stranded DNA molecules, I was wondering because you said ribose.
Yes you are correct! It should read "deoxyribose" since it is DNA sequencing... Well done for spotting it and thanks! :D
Shouldn’t we only add free nucleotides that aren’t the same type as the terminator bases? For example, lets say in a test tube we add the terminator base A, should the “ free nucleotides” added only be T,C,G? Otherwise the chain wouldn’t always terminate at the base A ?
Great video! Can I ask if you are going to a video on genetic engineering?
Yes it's been requested a few times so will be high on the list! :) Just need to have enough time to make videos... Thanks for watching!
Hey! Do you think you could make a video for chapter 21.3?
youre actually the best thankq so much every thing follow my book and make everything easier to understand
so helpful thank you!
Glad you find it helpful! Thanks for watching :D
Wow...thank you so much!!!
thank youuu!
do you think you'll be uploading any other videos today or tomorrow?
Sorry I don't think I'll be able to upload any more today... :( But I will be making more next week, so hopefully they can help you with paper 3...!
alright thank you very much :)
6:42 is this DNA polymerase the same as taq polymerase? can we say taq polymerase instead?
Yes, since many DNA sequencing processes are done in thermocyclers as well.
@@BioRach my nigga
@@sullym9383 😭
Ok so the pattern of bases on the nylon membrane is actually complimentary to the actual DNA molecule.
love!!!
ltrying earning the difference between profiling and sequencing ,
DNA profiling is separating DNA fragments based on their lengths, which is useful for identifying individuals based on their genetic profiles. You will see bands of DNA fragments with the help of gel electrophoresis.
DNA sequencing is finding the order of nucleotide/bases that make up an individual's genome. The result is a sequence of A/T/C/G.
@@BioRach This is going to sound like such an obtuse question but why does it need to be separated and sequenced if it is already in the sequence ?
i understand the process but for me to have the confidence to answer the more implicit and nuanced questions with answers you dont find in textbooks but rather initiative , i need to know why it has to be sequenced
also thank you for these two clear definitions
@leen908 You might not need this anymore but in case it helps anyone ....we don't know what the sequence is until we analyse the fragment lengths by electrophoresis. each individual fragment tells us the DNA base present at "that length" of the sequence (because each fragment ends in the corresponding terminator base), and since we have the full range of sequence positions, we have the base present at every position. if we order them by size, we get the entire dna sequence.
Just to confirm is it still Taq DNA polymerase being used here?
It's not really specified in the spec, but I would assume so as DNA sequencing is done using a thermal cycler (same as in PCR) which changes its temperature frequently. Therefore it'll need the Taq polymerase to withstand the high temperature. Hope this helps :D Thanks for watching!
BioRach great thank you very much
Good👍
Glad to be of help! Thanks for watching :D