Adhatoda Vasaka Leaf | How to Do Section Cutting & Microscopy of Vasaka Leaf | सेक्शन कटिंग ऑफ़ लीफ

Adhatoda vasica Leaf= How to Do Section Cutting and Microscopic Study (ENGLISH) By Solution Pharmacy • Adhatoda vasaca Leaf S...
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Leaf of Adhatoda vasica ( Vasaka ) is an important drug of Ayurveda, prescribed as an expectorant. For pharmacognosy and botany students it is very important to know the procedure for section cutting off any part of plant. To make a microscopic study we need to make a clear section of Adhatoda vasica ( Vasaka ). For the preparation of the section, we have followed following steps-
1- Collect fresh and clean leaves of Adhatoda vasica ( Vasaka )
2- Collect 04 watch glass which will be used in keeping the leaves of Adhatoda vasica ( Vasaka )
3- Place water, Safranin, Potato cube and again plain water in respective watch glass for Adhatoda vasica ( Vasaka )
4- Take a fresh leaf of Adhatoda vasica ( Vasaka ) and place it over the plain surface
5- now take a new and sharp razor blade to cut the leaf of Adhatoda vasica ( Vasaka )
6- Prepare the cubes of potato for further use for holding of Adhatoda vasica ( Vasaka )
7- Now cut the leaf of Adhatoda vasica ( Vasaka ) containing its midribs portion.
8- Transfer the cutted portion of Adhatoda vasica ( Vasaka ) in watch glass containing water
9- Now place that cut leaf of Adhatoda vasica ( Vasaka ) in between the opening of potato cubes.
10- Now cut the excess portion of leaves of Adhatoda vasica ( Vasaka ) through a razor blade.
11- Now move the blade through and forth direction
12- Place the leave of Adhatoda vasica ( Vasaka ) in watch glass containing water
13- Transfer the section of Adhatoda vasica ( Vasaka ) into a safranin contain watch glass
14- Now place that stained leaf of Adhatoda vasica ( Vasaka ) into watch glass containing plain water to remove excess color.
15- Place back the leaf into glass slide and add 2-3 drop of glycerin-water to prevent drying of Adhatoda vasica ( Vasaka )
16- Now add a coverslip over a slide containing leaf of Adhatoda vasica ( Vasaka )
17- Watch the leaf under a microscope and draw the structure.
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[@rajvanshiaditya]

First of all thank you madam for your guidance. But I'm still nervous regarding the samples I'll be getting in my practical exams, coz most of them are either infected leaf or bryophytes and therefore they are fragile and bit difficult to cut.

[@hareeshkumarg1087]

We never the usage of pith, so helpful mam, taking thin section was a challenge to us ,but not now 🙏🙏🙏

[@solutionpharmacy]

All the best

[@shitalkhade2388]

Very helpful vedio ma'am....and well explained also 😊😊

[@umeshtayde7598]

ye video bahot kam ayega madam thanx

[@Science.in.action1]

Thanku mam

[@lifescience9091]

very helpful

[@Sifer-m3w]

Nice

[@shivamkumarair4973]

Thank you mam

[@Drkhushigarg]

Very helpful 🙂

[@solutionpharmacy]

Thank you

[@solutionpharmacy]

Thank you

[@ravinapatidar9289]

Ma'am curry leaves k exam bhi same process h kr sukty?

[@MiniMovies011]

Ma'am can you give in pdf format in procedure

[@prernasharma3900]

Kaya aap D pharmacy ka new syllabus complete kariye naa pls

[@rinku99243]

👍👍👍

[@Adityabhatiwad]

Make a video on pdamja Uday Kumar medical pharmacology book plz

[@solutionpharmacy]

Refer pharmacology for Pharmacy students by Padmaja Udaykumar. Both are almost same

[@Adityabhatiwad]

@@solutionpharmacy sir I'm bsc nursing student

[@mehtabjan7776]

Hello sir I have a question??