Klebsiella (pink) and Pseudomonas on Macconkey agar of Sputum culture

When observing Klebsiella and Pseudomonas on MacConkey agar from a sputum culture, the key characteristics to look for are as follows:
MacConkey Agar:
It is a selective and differential medium designed to isolate Gram-negative bacteria and differentiate them based on lactose fermentation.
Lactose fermenters appear pink or red due to acid production, which lowers the pH and leads to the color change.
Non-lactose fermenters remain colorless or take on the color of the medium.
Klebsiella:
Klebsiella species, such as Klebsiella pneumoniae, are lactose fermenters.
On MacConkey agar, Klebsiella colonies will appear pink to red due to lactose fermentation.
The colonies are usually mucoid and have a glistening appearance due to the presence of a polysaccharide capsule.
Pseudomonas:
Pseudomonas species, such as Pseudomonas aeruginosa, are non-lactose fermenters.
On MacConkey agar, Pseudomonas colonies will appear colorless or take on a slightly yellowish or greenish hue due to their pigment production.
The colonies may have a metallic sheen and a distinctive fruity or grape-like odor.
Image Description:
An image of Klebsiella and Pseudomonas on MacConkey agar from a sputum culture would show two distinct types of colonies. The pink colonies represent Klebsiella, indicative of lactose fermentation. These colonies are likely to be mucoid and glistening. The colorless colonies represent Pseudomonas, which do not ferment lactose and may show a metallic sheen with a possible greenish tint due to pigment production.
Klebsiella,
Pseudomonas,
MacConkey agar,
Sputum culture,
Lactose fermentation,
Pink colonies,
Non-lactose fermenters,
Gram-negative bacteria,
Selective medium,
Differential medium,
Mucoid colonies,
Glistening appearance,
Polysaccharide capsule,
Colorless colonies,
Metallic sheen,
Pigment production,
Fruity odor,
Grape-like odor,
Klebsiella pneumoniae,
Pseudomonas aeruginosa,
Bacterial isolation,
Acid production,
pH indicator,
Bacterial differentiation,
Colony morphology,
Microbial identification,
Enterobacteriaceae,
Nosocomial infections,
Pathogenic bacteria,
Antibiotic resistance.