What is phase contrast microscopy?

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  • Опубликовано: 13 янв 2025

Комментарии • 22

  • @ahmetkaracaoglu5617
    @ahmetkaracaoglu5617 10 месяцев назад +1

    Very useful and nice sharing.
    I am very happy to come across your videos on RUclips.
    Thank you very much.

  • @MarieChardome
    @MarieChardome 10 месяцев назад

    thank you for this. it highlighted the use of PC, showed how DIC is way nicer and i can't wait for oblique illumination

  • @MarieChardome
    @MarieChardome 10 месяцев назад

    @MH, regarding the blurry 'aura' around objects in PC: does a higher objective, eg 100 oil, magnify everything? or does it have mechanisms inside that allows it to magnify the object, but not the phase aura exponentially? too farfetched?

  • @tinimunson1242
    @tinimunson1242 10 месяцев назад

    hi, will you go live on Saturday this week like normal? I don't see the announcement.

    • @Microbehunter
      @Microbehunter  10 месяцев назад +2

      Yes, I will go live as normal (Satruday). I prepared several pollen slides with different mounting media and I also set up a second microscope to observe using darkfield.

  • @philhunt1442
    @philhunt1442 8 месяцев назад

    will my Olympus ck2 phase contrast microscope give similar results?

  • @warmgreetings
    @warmgreetings 10 месяцев назад

    @MH- Hi😊
    Would you please consider showing the fluid from your eye 👁️ 👁️ under the microscope 🔬?
    Your saliva sample was so interesting 🤩

  • @lotharmayring6063
    @lotharmayring6063 10 месяцев назад

    has the lambda shifting ring to be located after objectiv or as well in condensor diaphragma and what efect would this cause Can i use mica plates for the lamdaquarter shift. Is the gray colour of the objectivring only for aligning or has it other purpose. If you really are an expert of microscopy understanding really phasecontrast you should be abel to answer my questions. i am waiting

    • @MarieChardome
      @MarieChardome 10 месяцев назад +1

      bro: watch your tone ay.

    • @lotharmayring6063
      @lotharmayring6063 10 месяцев назад

      @@MarieChardomeno because this amateur guy never responds my questions , he sells microscops ay

    • @MarieChardome
      @MarieChardome 10 месяцев назад +2

      @lothar: 1. please be patient, he cannot always answer all our questions right away. 2. your stuff seems pretty interesting and advanced. maybe you could write him an email? 3. this is an amateur microscopy channel and never pretended otherwise 4. i learned so much on this channel and it gave me a whole new direction. i am extremely grateful. if it's not your scene, let us be happy 5. i don't give a toss about him selling things or not: it is his time and effort and money that gives us all this free information. 6. he's the best teacher i ever had. talking like he sits on the other side of your table, unlike on some other nice channels where they use unnatural voices. you will not ruin our happiness with your rather agro and demanding behaviour. please be kind. i wish you an awesome microscopy journey 🙂

    • @lotharmayring6063
      @lotharmayring6063 10 месяцев назад

      @@MarieChardome1. there are no amateur microscopers, this is his own invention
      2. at the beginnig i liked his videos but now he is making a bad sutterday late night show
      and is telling always the same, trying to make publicity for his microscop shop
      3. he calls himself professor, but in which university is he teaching
      4. he often tells nonsene and, i have written hundrets of comments but he is not able to respond one
      5. there are much mor better sides on youtube like microcosmos
      6. he makes very bad preparations and even his pictures aren good
      7. i like to critisise him and telling people the false information he gives and dont like people who alwas say
      ,,,how good is this micobehunter-profe
      8. i work over 50 years in microscope also at university and have my dr. rer, nat . i have over 20 Microscopes and many publication

    • @lotharmayring6063
      @lotharmayring6063 10 месяцев назад

      @@MarieChardomeplease be more objectiv and answer my questions instead of blah blah of the manner i am talking. I dont like peopel who always say.. oh how good is this profe

  • @lotharmayring6063
    @lotharmayring6063 10 месяцев назад

    the most important of phase contrast is the phase shift of the specomaen wave front done by objectivs phase ring. This profe tells us nothing about that he even did not mention it

  • @lotharmayring6063
    @lotharmayring6063 10 месяцев назад

    why do you not compare obique illumination to phase contrast...........would make much more sense

  • @lotharmayring6063
    @lotharmayring6063 10 месяцев назад

    here microbehunter tells us nothing of the principle of phase contrast. He onyly tells us some technical stuff of his microscopes and shows us the care phasecondensors which really are not necessary acheving phase contrast. What you really need arr those phase ringdiaphagmas . You cand make them DIY and put them in a normal condensor. of course for each objective. Remember that th outer circle of the ring can be the Koehler diapharagm

  • @lotharmayring6063
    @lotharmayring6063 10 месяцев назад

    why does he always make this publicity for Olympus.......not only japanese make microscops

    • @Microbehunter
      @Microbehunter  10 месяцев назад

      Because I happened to have phase contrast condensers for Olympus. This is the system that I use.

    • @lotharmayring6063
      @lotharmayring6063 10 месяцев назад

      @@Microbehunterok my friend, that i could imagine me.
      But i do not understand why you show this absolutly unnessesary and super care
      so called phase condensors and so mislead peopel to buy them.
      What you need are only ring diaphragmas which can easly made by DIY.
      Your phasecontrastcondensors are only inventions of th microscop companies
      and serve to make money. Zernicke never used them for his methodd.
      As wel unnessesary are this centering phase telescops for about 100 bugs.
      Would be better to teach how to DIY a phase plate for the objektiv...
      How do they make them...

    • @lotharmayring6063
      @lotharmayring6063 10 месяцев назад

      @@Microbehuntershit happens

    • @lotharmayring6063
      @lotharmayring6063 10 месяцев назад

      @@Microbehunterok my friend, that i could imagine me.
      But i do not understand why you show this absolutly unnessesary and super care
      so called phase condensors and so mislead peopel to buy them.
      What you need are only ring diaphragmas which can easly made by DIY.
      Your phasecontrastcondensors are only inventions of th microscop companies
      and serve to make money. Zernicke never used them for his methodd.
      As wel unnessesary are this centering phase telescops for about 100 bugs.
      Would be better to teach how to DIY a phase plate for the objektiv...
      How do they make them...