- Видео 12
- Просмотров 386 294
Rejo Jacob Joseph
Добавлен 2 сен 2013
Coagulase Test
The coagulase test is used to differentiate Staphylococcus aureus from other Staphylococcus species like Staph. epidermidis and Staph. saprophyticus on the basis of the ability to produce the coagulase enzyme.
Просмотров: 21 919
Видео
LPCB Tease Mount & Scotch tape preparation
Просмотров 22 тыс.3 года назад
This video demonstrates LPCB tease mount & sellotape Flag preparation in mycology to examine the fungal structures. Lactophenol cotton blue stains the fungal cytoplasm and provides a light blue background against which walls of the hyphae can readily be seen. www2.slideshare.net/RejoJacobJoseph/mycology-colony-morphology-microscopy-part-1
Streak plate culture method
Просмотров 3,6 тыс.3 года назад
This video demonstrates the streak plate method for isolating a single colony of bacteria from a solid and liquid culture with the help of a nichrome wire loop and a disposable plastic loop. www2.slideshare.net/RejoJacobJoseph/gram-positive-cocci-identification-part-1 www2.slideshare.net/RejoJacobJoseph/identification-of-gram-negative-bacilli-part-1-239370906
Agar well diffusion assay
Просмотров 127 тыс.3 года назад
Sensitivity test by well diffusion method. www2.slideshare.net/RejoJacobJoseph/antibiotic-susceptibility-test-ast-239030380 www.slideshare.net/RejoJacobJoseph/antibiotic-sensitivity-test-by-dilution-method
E-Test (Epsilometer Test)
Просмотров 41 тыс.3 года назад
E-test is a laboratory test used to determine the minimum inhibitory concentration (MIC). This video demonstrates how to perform and interpret E-test antibiotic susceptibility. www2.slideshare.net/RejoJacobJoseph/antibiotic-susceptibility-test-ast-239030380
Antibiotic Sensitivity Test by Disc diffusion method (Kirby Bauer method & Stokes method)
Просмотров 121 тыс.3 года назад
This video demonstrates how to perform a disk diffusion test includes Kirby Bauer and stokes method. This method is used for testing the susceptibility of bacteria and fungus to different antimicrobials. www2.slideshare.net/RejoJacobJoseph/antibiotic-susceptibility-test-ast-239030380 www2.slideshare.net/RejoJacobJoseph/antibiotic-sensitivity-test-by-dilution-method
Oxidase Test
Просмотров 14 тыс.3 года назад
How to perform oxidase test with the oxidase paper strip method and by oxidase reagent. The Oxidase test is used to differentiate oxidase-positive bacteria from oxidase-negative bacteria. www2.slideshare.net/RejoJacobJoseph/identification-of-gram-negative-bacilli-part-1-239370906
Catalase Test
Просмотров 2,2 тыс.3 года назад
The catalase test is used to check the presence of catalase, an enzyme that breaks down the hydrogen peroxide into water and oxygen. www2.slideshare.net/RejoJacobJoseph/gram-positive-cocci-identification-part-1
Gram Staining
Просмотров 6523 года назад
This video will cover what is gram staining, what are the reagents needed for gram staining, and its action, and how to perform Gram staining? www2.slideshare.net/RejoJacobJoseph/different-staining-techniques-in-bacteriology-238562953
Bacterial Smear Preparation and Simple Staining
Просмотров 30 тыс.3 года назад
How to prepare a good bacterial smear from solid and liquid cultures. How to perform a simple stain
Compound Light Microscope Parts & How to focus using Oil Immersion lens
Просмотров 9703 года назад
This video helps to learn the parts of the compound light microscope and how to focus using on a 100x or oil immersion objective lens properly.
Bacterial and Fungal Exhibition
Просмотров 1,6 тыс.4 года назад
Catalog of Bacterial and Fungal lab protocol
Thanks a lot sir
Wht was the use of 0.5 mc farland standard😢
www2.slideshare.net/RejoJacobJoseph/antibiotic-susceptibility-test-ast-239030380
Thank you for sharing, I finally feel enlightened
How many colonies do we need to pick ? Thanks
How to perform antibacterial activity of CuO nanoparticles
Which chart did you use as reference please and where can I download it?
Why the bacterial suspension is compared to 0.5 Mc farland standard
It helps to ensure that the number of bacteria will be within a given range to standardize microbial testing.
This really helps me a lot in carrying out sensitivity test in my lab
You need to streak in 4-5 directions, not 2
Sir in agar well addition of melted agar drop is necessary
For better results add 1-2 drops of melted agar to seal the lower border of the well..
Hello everyone, I want to know is this test identify the ESBL bacteria or I have to do more tests.
Sir in comments ,you said that if the extract is viscous ,we dont need to add melted MH agar at bottom, in your experiment also the extract is viscous then why you added MH agar on bottom?
Sir,Which is media is used for sauropus androgynus plant extract for antibacterial test?
For antibacterial test better use MHA or MHA with blood
Sir when I pour my extract into well then zone of inhibition is not clearly visible due to color of extract then what I do please suggest
Before adding the extract, seal the underside of the well. When adding the extract, ensure not to exceed 100 microL. After incubation, if no clear zone is observed or if bacterial growth surrounds the well, it indicates that the extract concentration is insufficient........
@@rejojacobjoseph okay sir thank you for your suggestions
Can nutrient agar plate be used?
Better use only MHA
Which fungus is this????
I think aspergillus
Sorry, can you write the method here?
Tq sir
It's really helpful..Thank you so much sir
Hello sir I'm inter 2 year student can you please tell me this mothad: tube dilution and agar dilution please explain me sir
www.slideshare.net/RejoJacobJoseph/antibiotic-sensitivity-test-by-dilution-method
Hi what is the diameter of the well using blue pipette tip
Is incubation not required if bacterial suspension is prepared using MH broth, as with glucose broth or peptone water where the suspension is incubated before making a lawn
Incubation is required after inoculation
Nice thanks sir
hello, thank you for the video! . I want to ask, how do you choose the positive control?
U can select one commonly used susceptible antibiotic for the inoculated bacteria. eg. For staph aureus u can use erythromycin as PC.
@@rejojacobjoseph thank you
Dear sir How to measure the ZOI in agar well diffusion method. since incase of Disc Diffusion assay the ZOI is measured end to end which also includes the area under the disc. in this case there is a well and couldnt be counted as clearing zone
Measure same like disc diffusion method if the zone is clear.
What is the purpose of the saline in the tube method?
Sir helpfull thanks 👍
Can this method work for 2cm or 2mm fabric test
How can we take reading of 2mm or 2cm fabric by this method
Thank u sir
Sir can you please make video on aloe gel extraction
hello sir, i am working on fungus but I am unable to culture it because of contamination, and how else can I try to culture and isolate fungus from seed cake surface.please help by suggesting something
U can transfer the fungus to the media by loop. When fungus grows, u can see different colours (means different type of fungus). With a sterile loop Touch only on to the top (spores) of one type of fungus (touch only 1 color) and transfer to the media and incubate the plates in an inverted position.
@@rejojacobjoseph yes sir I've tried that. Any more techniques or ways to culture it?
And it's turning out to be milky in consistency
Clear and easy to understand, relaxing as well! Thank you!
Wonderful. How do I get the interpretation charts for the antibiotics. Any help will be much appreciated. Thanks
Interpretation chart u will get it from the antibiotic company site / u can enquire with the suppliers.
Hello my products are neem extract nutmeg extract combo of neem & nutmeg extract herbal gel & marketed preparation. My need extract is viscous with what solvent I should dilute with?? Tween 80?? Can't I add above products directly?????
Plz check some article related to neem extract study. So that u will get an idea about it..
should the plate place inversed or not when incubated?
Sir pls also upload video of poison food method
Pls do reply me sir
Thanks for helping me understand heat fixing.
Sir is this entire procedure done inside luminar air flow
Yes..All the works must done in the safety cabinet only.
I love u brother ♥️♥️
Sir, I want to know, when one is using a plant extract, unlike conventional antibiotics that have a standard, how can one determine the concentration of the plant extract that inhibits the bacteria when using MIC
Thank you for the video sir. What control disc have you used in this video sir? I have a similar experiment with curcumin nanoparticles, but they are in paste form, not liquid form, do you have any suggestion in how I should load my sample inside the wells?
When u do this procedure first u add full con: of ur extract in to the well and check the zone of inhibition and compare the zone with antibiotic zone (commonly used antibiotic used for the bacteria u inoculated). If the zone is big then u can perform the MIC/ dilution and again repeat the procedure to get a good result with less dilution.
@@rejojacobjoseph thank you for the response sir
Tks for the video! What dilution for plasma do you use?
ruclips.net/user/shortsWNWQcn9IcG4?feature=share
What will be the distance between two disc in 110 mm Mueller Hinton Agar?
What is the distance between the disc and the periphery?
Awaz boht slow hai.
Yup
Thanks so much
What is the composition of Muller Hinton Agar media?
Greate explanation thank you