Congratulations. A very professional and highly educative shot. I never met Bursaria in my high salinity samples from which I extract my videos. I wish to be the same lucky to capture Fabrea salina cysting but as I use hypersalinity it soon turns to salt and ruins my sample. When Bursaria excysts it leaves behind a kind of shell remnant? Is that so? I am very impressed by the way you direct your shooting. One of the best videos I ever saw.
Thanks George. By the way: Remnant with a hole = Bursaria. Prorodon very has similar cysts, but without a (predefined) hole, and they burst their cysts. I did not observe the excysting of Prorodon, will continue trying, at learst occasionally. With Bursaria, it took me approximately 1.5 years :-)
Amazing! I've been trying to find my Bulsaria for 3 weeks and I just stumbled upon them today when I looked at my samples and saw a larger than usual microbe swimming around. At the beginning of the video you had the Bulsaria being biolumicent, how did you do this? What type of light(Phase Contrast, Dark field, so on) was it and did you use any specific disks?
@@KambizMT Very cool! I don't mean to ask for too much but perhaps you could make a quick video of how to make those disks?(Even showing me what they look like is enough) I'd very much like to see in that type of dark field..I hope you can do it!
@@Suffex_1 I simply used a disk and put it on the condensor. Best diameter of the disk for my microscope, at least, was 12 mm. I did not use it for a long time, now, not sure where I put it :-). But, there are nice videos on RUclips. I hope this helps, good luck!
Very impressive optical quality; covers much of the gamut of activities of Bursaria in its life cycle. You should consider adding narration -- it is much work to do so, but adds educational value and answers questions before they are asked. I have recently also posted a program on Bursaria truncatella on RUclips which you may find of interest. Best wishes, GP Sartiano, M.D.
Thank you! Yes, plant infusions, based on this publication: Beers, C. Dale, 1948: Excystment in the ciliate Bursaria truncatella. Biol Bull: 86-98. Unfortunately, it´s still some kind of try and error. If you want to try it, too: it seems that the strong illumination of the microscope delays, or may even prevent excysting. Similar with the encysting, which is less difficult to be catched. But, if you start illuminating/recording too early, the process does not proceed. It seems that the organisms must have passed a specific point of no return, and then proceed, inspite of the illumination, to complete excysting and encysting, respectively.
Hi Riccardo, as Aaron mentions, it´s no symbiosos. The long organisms are paramecium, normally eaten by Bursaria. At 5:50 that is after Bursaria lost it´s shape, got through cell division, and did not gain it´s shape again, yet. It is not fully differentiated, and the bursaria finds it´s way out, again. Otherwise, it would have been eaten as shown at 1:42
Excellent.
Great editing to capture the whole life cycle of these organisms.
The use of timelapse helps cover all this and all in 6 minutes. Nice!
Congratulations. A very professional and highly educative shot. I never met Bursaria in my high salinity samples from which I extract my videos. I wish to be the same lucky to capture Fabrea salina cysting but as I use hypersalinity it soon turns to salt and ruins my sample. When Bursaria excysts it leaves behind a kind of shell remnant? Is that so?
I am very impressed by the way you direct your shooting. One of the best videos I ever saw.
Thanks George.
By the way: Remnant with a hole = Bursaria. Prorodon very has similar cysts, but without a (predefined) hole, and they burst their cysts. I did not observe the excysting of Prorodon, will continue trying, at learst occasionally. With Bursaria, it took me approximately 1.5 years :-)
Amazing!
I've been trying to find my Bulsaria for 3 weeks and I just stumbled upon them today when I looked at my samples and saw a larger than usual microbe swimming around.
At the beginning of the video you had the Bulsaria being biolumicent, how did you do this? What type of light(Phase Contrast, Dark field, so on) was it and did you use any specific disks?
Thank you!
At the beginning, that is dark field, with selfmade disks.
@@KambizMT Very cool! I don't mean to ask for too much but perhaps you could make a quick video of how to make those disks?(Even showing me what they look like is enough) I'd very much like to see in that type of dark field..I hope you can do it!
@@Suffex_1 I simply used a disk and put it on the condensor. Best diameter of the disk for my microscope, at least, was 12 mm. I did not use it for a long time, now, not sure where I put it :-).
But, there are nice videos on RUclips. I hope this helps, good luck!
Kambiz, this is exquisite. What microscope do you use? What are the little species interacting with the larger one?
Hi Dennis, Thanks! I use Zeiss Standard 16. The little species are Paramecium, which seem to taske quite well.
Very impressive optical quality; covers much of the gamut of activities of Bursaria in its life cycle. You should consider adding narration -- it is much work to do so, but adds educational value and answers questions before they are asked. I have recently also posted a program on Bursaria truncatella on RUclips which you may find of interest.
Best wishes,
GP Sartiano, M.D.
Thank you for posting this. These are amazing creatures.
Did you use a medium for their excysting? Fantastic videos.
Thank you! Yes, plant infusions, based on this publication: Beers, C. Dale, 1948: Excystment in the ciliate Bursaria truncatella. Biol Bull: 86-98. Unfortunately, it´s still some kind of try and error. If you want to try it, too: it seems that the strong illumination of the microscope delays, or may even prevent excysting. Similar with the encysting, which is less difficult to be catched. But, if you start illuminating/recording too early, the process does not proceed. It seems that the organisms must have passed a specific point of no return, and then proceed, inspite of the illumination, to complete excysting and encysting, respectively.
@@KambizMT Thank you so much for your reply. Sounds like fun. I have a bunch of cysts that I am planning to excyst. Your tips would greatly help
2:50 are they living in symbiosis? Looks like the long things are helping the circle ones to divide
5:50 ?
No, there are there own organism
Hi Riccardo, as Aaron mentions, it´s no symbiosos. The long organisms are paramecium, normally eaten by Bursaria. At 5:50 that is after Bursaria lost it´s shape, got through cell division, and did not gain it´s shape again, yet. It is not fully differentiated, and the bursaria finds it´s way out, again. Otherwise, it would have been eaten as shown at 1:42
@@KambizMT thank tou, cool video!