How to Change Cell Culture Media

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  • Опубликовано: 26 янв 2025

Комментарии •

  • @science_mbg
    @science_mbg 8 лет назад +37

    You should need to edit the title or add in description section about the cell type whether it is attached or suspension cell line. Because if it was suspension cell line, all the cell would be discarded as well by removing the media at first place.

    • @정주안-k8d
      @정주안-k8d 4 года назад

      i agree with you

    • @cansutacettin3024
      @cansutacettin3024 3 года назад

      totally agreed!

    • @funny11744
      @funny11744 9 месяцев назад

      Do you have any idea how to isolate the URNIE STEM CELLS by simple centrifugation? Usually, the stem cells are mixed with sediments in the morning urine- the best time for obtaining the urine stem cells. I resuspended the sediments -which disappeared at the next centrifugation , but it did not help to find any single urine stem cell. Usually, there are just 5 to 10 urine stem cells in 100 ml of fresh urine. I used a protocol with 500 g for 5 minutes of urine centrifugation.

  • @JYOtiRaNJanMANgaRaj
    @JYOtiRaNJanMANgaRaj 9 месяцев назад +1

    Thank you so much 😊

  • @osmirod1
    @osmirod1 2 года назад +2

    The t75 must remain with the cap on at all times except when pipetting

  • @el-yugudaabdul-dahiru39
    @el-yugudaabdul-dahiru39 9 лет назад +2

    The video is educative and interesting. However, if you are not of maintaining the sterility or not too sure of your working environment please revert to using flame at every point of your work.

  • @retna2183
    @retna2183 12 лет назад +1

    KS LEE you mean open the lid slightly of the T-75, after placing in the incubator for air flow? It may be the T-75 where the cap has a filter, so you do not have to loosen it.
    Cheers

  • @FireboltRiver
    @FireboltRiver 11 лет назад +5

    Shouldn't he spray his hands again with alcohol whenever he discards used pipettes under the cabinet?

    • @jeeva1996
      @jeeva1996 4 года назад +1

      No, that quick movement is fine

  • @kyoung731
    @kyoung731 11 лет назад +5

    There's no need for PBS if it's you're just doing a regular media change.

  • @ibraheemomar8109
    @ibraheemomar8109 9 лет назад +4

    why are you not wearing a face mask?

  • @moonlikeyou3339
    @moonlikeyou3339 Год назад

    How long do you change the medium?

  • @12823matthewkao
    @12823matthewkao 3 года назад +2

    you didn't wash the culture with PBS before adding the fresh media?

  • @tulsijoshi1046
    @tulsijoshi1046 Год назад

    If she discarded the media won't the cells go with the media?

    • @eversincekorea
      @eversincekorea Год назад

      the cells are adhered to the flask

    • @funny11744
      @funny11744 8 месяцев назад

      Only the dead cells go with media, the adherent cells are fine. Which is good for the healthy of last one. I think.

  • @funny11744
    @funny11744 4 года назад

    Please mention what type of cells are ? Are human cell ?

  • @amrutasudake7271
    @amrutasudake7271 6 лет назад +1

    In how many days we should change media

  • @ubanakoblaise9138
    @ubanakoblaise9138 11 лет назад +2

    No need to wash cells with PBS?

  • @kslee8096
    @kslee8096 12 лет назад

    Why not open partially the T-75 in the CO2 incubator in the last part of the video?

    • @kannanrk100
      @kannanrk100 7 лет назад +1

      KS Lee it's a vented flask

  • @HuongPham-ur1sz
    @HuongPham-ur1sz 11 лет назад

    làm sao để xem dc đây

  • @toshibacoast
    @toshibacoast 13 лет назад

    good video.

  • @SubhadeepD
    @SubhadeepD 9 лет назад

    great video indeed :)
    am just start doing my phd work .... and this video will help me :)
    sterile work = great data/result :) cheers :)

  • @vadersfist12
    @vadersfist12 11 лет назад +6

    what kind of bracelet was he wearing? is it sterile? I think not... for shame, sir. for shame.. :( not :D

  • @lemyafinder289
    @lemyafinder289 8 лет назад +1

    i'm looking for a cell culture lab for an internship..can any one help?

  • @abumosabelhindi5505
    @abumosabelhindi5505 12 лет назад

    its very nice

  • @Carl-Ernst-Otto-Kunze
    @Carl-Ernst-Otto-Kunze Год назад

    @Zauberberg

  • @Gangularis
    @Gangularis 8 лет назад +2

    Why are you wasting your time spraying the pipette packaging? You aren't
    going to get it close to being completely sterile, and that wrapper
    should never be over any of your open containers, anyway.

    • @funny11744
      @funny11744 8 месяцев назад

      just for precaution.

  • @vadersfist12
    @vadersfist12 11 лет назад +1

    My bad I meant to dislike this. CSU Pomona does it hood status!

  • @infinitefuture1828
    @infinitefuture1828 3 года назад

    You shouldn't wear socks while performing experiments

    • @funny11744
      @funny11744 8 месяцев назад

      it depends on how skilled you are and it depends on the cells.

  • @vadersfist12
    @vadersfist12 11 лет назад

    CSU Pomona does it better!!!