If the sunscreen is zinc based, they should be fine. If the sunscreen is carbon based, the UV will just heat it up and they’ll either incubate or die trying.
Why is there so many people talkin 'bout gloves? I work in a microbiology lab on pharma and we do not wear gloves. It' s not like splashes or something. It's all about knowing how to handle them. I believe you did it just fine. Normaly people who's afraid without knolwedge of bacterias get scared.
Yeah, in school, even when I worked with piranha solution or aqua regia I didn't really wear gloves. The only time we did was for hydrogen fluoride. If I didn't need to wear gloves for aqua regia you probably don't need to wear gloves for E. coli, especially when there's already a shit ton on your skin.
@@mango251 that funny, you know Flexseal really liquid Rubber. UV light eats and destroys elastic polymer Rubber. Rubber enemy is UV light. Don't care if it's vulcanizing like tires they degrade. That why sunscreen like a Rubber protection cream lotion. You are actually protecting the dermis, elastin elastic rubbers band's fibers in our skin, from degradation and wrinkles. Our skin, flesh matter or whatever you call it is similar to a Rubber coverage, over our body's. The pigment melanin a natural sunscreen is natural built-in our DNA. But what most don't understand is, the more skin pigmentation we have the more the skin is tighter lest ability to stretch, the less pigmentation and dna of lighter skin types the more elastin rubber like the skin is. That the way DNA ,dermis etc is , but UV will destroy Flexseal🤣😃😄😄, and Rubber and skin.
How did you quantify the amount of bacteria left over? Like is there a way to compare the growth of the control side and experimental side? Not sure if that makes sense, but thanks
Hi Katie, Usually you can count the number of bacterial colonies and compare the different sides. Highly concentrated cultures often leave a "lawn" of bacteria which we can see in the control side. Hope that helps.
You can literally see the living bacteria with the naked eye. Couldn't you see the difference between the sides? And he was just counting how many separate groups (colonies) were remaining. Just the number of colonies and total size (area, because they're on a very flat surface) should be all we care about.... not the actual number of bacterium left over.
What if after being exposed the the UV light, you look down is shock as the E. coli takes of a pair of shades and says “Hey! Thanks for this nice, golden tan!”
John Olson it does work, but it also kills everything else. Using UV light to treat Covid in a person makes about as much sense as boiling them to sterilize them.
I honestly love watching bacteria colonies grow, it’s my favorite thing to watch, it’s interesting to see this though. It reminds me of my science fair project, how much bacteria does make up contain? I had a lot of fun watching the progress of the bacteria colonies and seeing them grow, it was very fun. I would like to try this one day at my schools lab, maybe with something different.
Anyone else freaking out about him not using gloves while handling the E. coli? Lmao. My microbiology teacher always made sure we handled our Petri dishes with gloves.
Thanks the video is a boon to explain community the effect of time and UV radiation on harmful ecoli found in so many water and food items we consume daily.
I know this may sound a bit insane, but if only we could do this same experiment but with even stronger EM waves to test how long microbes could withstand, like say, X-Ray.
@@sci-inspi I'm not a biologist so idk if increasing light intensity would decrease disinfection time. But, I do know that uv follows the inverse square law so the light should be far more intense the closer it is.
@@Dysfunctionality15 I think it's also depend on UV wavelength, from all I know, 260-270 nm is the best to kill E.Coli. The lower is, the less effective. I'm not sure what happend if UV wavelength is higher.
I have thought of an experiment involving this method. I'll cover a plastic wrap above the petri dish after inoculation where I can apply a handful of sunscreen. In theory, this should filter, or at least some, UV rays; thus having more growth compared to those completely exposed to UV. It would be nice if you have an insight to this matter.
thanks I have 13w uv light.. I submerge it into my water dispenser for drinking.. I was wondering how long to turn on the disinfection cycles.. now I have an idea..
This is what I was also wondering. Ozone is heavier than air so it will fall down onto the uncovered side of the petri dish. Perhaps they could seal the dishes and remove all oxygen.
So to protect myself from bacteria I just have to stand under an extremely intense UV light source for extended periods of time. Maybe if UV kills bacteria, it can also kill skin cancer. I think I just earned myself a noble prize.
Eh, the bacteria used in a lot of labs are non pathogenic, and if you use proper sterilization, handling, and clean up techniques it’s fine. You can use gloves for extra protection though (I generally do because I’m clumsy sometimes)
the video is 3 years old, but I really liked it, I just missed seeing the PPE protection in your hands when handling the bacteria, or with your eyes in the ultraviolet light. I'm from Brazil and this video will help me do some research
hii, you have got a good video. would you do a video on Ozon air toward bacteria and mold pls ? there are lot of cheap ceramic ozone generator on ebay.. thanks andrew
I hope you wore proper eye and skin protection when using UV-C bulbs! Even brief exposure can cause temporary damage, and anything more than a few seconds can cause burns in your eye. Apparently it feels like you have sand in your eyes all the time... some people's eyes seem to recover, others have irreversible damage. All depends on length of exposure and the power of the lamp. Best to just not take any chances. Even standard polycarbonate safety glasses cut pretty much 100% of UV-C light but even though I know it's factually true it still doesn't feel safe to me and I tend to use specialized UV blocking glasses. For those wondering, the reason UVC is so dangerous to humans is that we are never exposed to it naturally. The sun does produce quite a bit of UVC but the atmosphere filters almost all of it out, as well as most UVB. The UVB that gets through is what causes tans/sunburns/melanomas. UVA does also play a part but it's not nearly as damaging as the shorter wavelength UV.
Where do you get your bread from lol.. You don't have to do that if it's fresh bread, bacteria is killed within the oven.. But even if there is some bacteria left, and someone touches the bread, it can build your immunity to that bacteria.. If you disinfect every single food you eat, you wouldn't have immunity and you would easily get sick just by going out
can anyone tell me what is the best uv wand to get i want to make sure it's not a knock off and it kills the bacteria and i want one i can change the bulb if possible for toilet bed sheets and kitchen counter and my lazyboy. how would a person go about testing if it kills germs as it should.?
it s not good to use UV light to kill bacteria in closed area like your toilet, because Uv light reacts with Oxigen O2 in the air to form ozone O3, which is toxic!
i recieved a light in the mail with no instruction it is led and it is blue it showed how what surfices needed to be cleaned I think it is a black light. Is there such thing as a led uvc light?
+Hung Tr Ozone has no smell, this thing we are feeling is nitrous oxide and this is real threat. Because ozone is hyperactive then easy oxidate nitrogen.
Not mentioned here that the UV light needs to be UV-C light. Not UV-A or UV-B. A or B will not kill bacteria or viruses. That’s a very important note not mentioned.
I am not sure. I used a lamp that had two 15 watt bulbs for this video. My guess would be the 3 Watt lamp would require more time to kill the bacteria. If you have access to a microbiology lab, you may be able to test the 3 watt lamp it.
what if all the bacteria just ran away to the other side?
E. coli can't move on a solid medium. It would take them a very long time to move to the other side as well.
Sci- Inspi Not to mention that not all strains of E. coli are motile. Perhaps you could have used a nonmotile strain.
@@sci-inspi r/woosh
@@davidgamingremastered6784 r/notawooosh
@@davidgamingremastered6784 r/plzcommitapoptosis
What if bacteria apply sunscreen..???
It should protect them. If the chemicals in the sunscreen aren't bad for them in the first place.
If the sunscreen is zinc based, they should be fine. If the sunscreen is carbon based, the UV will just heat it up and they’ll either incubate or die trying.
Absolutely brilliant 👏👏👏👏👏👏
Let’s just hope no bacteria’s read this comment or else we’ll have a real problem on our hands.
@@sci-inspi I love how you respond to these questions that were meant to be a joke. Really shows how there are no stupid questions.
'Bacteria' is already plural
Why is there so many people talkin 'bout gloves? I work in a microbiology lab on pharma and we do not wear gloves. It' s not like splashes or something. It's all about knowing how to handle them. I believe you did it just fine. Normaly people who's afraid without knolwedge of bacterias get scared.
Larissa Amores umm! maybe because...its E-coli.
@@whendeathdeclareswar7458 Do you know anything about E. coli besides "It makes you very sick"?
You are a part of the problem Larissa describes.
@@whendeathdeclareswar7458 how you shit
if he gets e coli on his hand he could just wash it off
Yeah, in school, even when I worked with piranha solution or aqua regia I didn't really wear gloves. The only time we did was for hydrogen fluoride. If I didn't need to wear gloves for aqua regia you probably don't need to wear gloves for E. coli, especially when there's already a shit ton on your skin.
You should spread the colonies who survived into a new petri dish and repeat the process until they all survive.
That's a good way to demonstrate adaption and evolution! Thank you.
Lol
Yep
That's a good way to create a damn superbug, don't do it!
To create a new super bacteria. No, don't do that.
Germs: 'exist'
Light: im gonna end this man's whole career
The sun is a deadly laser
Did he just write them on his death note or something?
Low Quality Shitposts not anymore there's a blanket
A bacteria isn't a humanoid, if you are gonna meme do it properly. T_T
love how he answer most of the comments
I try my best
@@sci-inspi Great video. Can you buy a regular lamp and put these bulbs in them?
Would be interesting to see the comparison between this video and other types of UV light being used
When Thanos even wipes out half of bacteria
Wayy underrated
noes noes PÉGATE UN TIRO
Hold up bacteria! it is i THANOS
SkiLLsZ u beat me to it,
Your profile picture make it better
upvote
Excellent video! I was going to make a video showing UVC light tests using salmonella, but no need to now.
_"To show you the power of UV light, I covered this specimen in half!"_
Protect yourself against the might of UV light with Flex Seal!
that is a laboratory UV source, more powerful than the battery UV-C wands.
@@mango251 that funny, you know Flexseal really liquid Rubber. UV light eats and destroys elastic polymer Rubber. Rubber enemy is UV light. Don't care if it's vulcanizing like tires they degrade. That why sunscreen like a Rubber protection cream lotion. You are actually protecting the dermis, elastin elastic rubbers band's fibers in our skin, from degradation and wrinkles. Our skin, flesh matter or whatever you call it is similar to a Rubber coverage, over our body's. The pigment melanin a natural sunscreen is natural built-in our DNA. But what most don't understand is, the more skin pigmentation we have the more the skin is tighter lest ability to stretch, the less pigmentation and dna of lighter skin types the more elastin rubber like the skin is. That the way DNA ,dermis etc is , but UV will destroy Flexseal🤣😃😄😄, and Rubber and skin.
How did you get italics?
How did you quantify the amount of bacteria left over?
Like is there a way to compare the growth of the control side and experimental side?
Not sure if that makes sense, but thanks
Hi Katie,
Usually you can count the number of bacterial colonies and compare the different sides. Highly concentrated cultures often leave a "lawn" of bacteria which we can see in the control side. Hope that helps.
ImageJ is also typically used so you can measure the area of patches or lawns of bacteria
You can literally see the living bacteria with the naked eye. Couldn't you see the difference between the sides? And he was just counting how many separate groups (colonies) were remaining. Just the number of colonies and total size (area, because they're on a very flat surface) should be all we care about.... not the actual number of bacterium left over.
I don't think the OP took basic biology class >.>
What if after being exposed the the UV light, you look down is shock as the E. coli takes of a pair of shades and says “Hey! Thanks for this nice, golden tan!”
Wait so that's not normal?
@U hahahahahahahahahhah
i saw u on the Lamborghini video too
love these scientific demonstrations!
Nice! E.coli! I like to spread that on my cheese peenut butter sammich
And how long you are eating shit?
@@4ipon4ik lmfao
*you are scaring the bacteria! Shush!*
Are you sure that it's peanut butter?
@@4ipon4ik that's a r/whooosh
Afraid you have E. Coli on your skin!? No worries! Lay under a UV lamp for a few minutes. You'll be fine.
Coronavirus:uhhhh why i can't infected you!!!?!
Me with full uv armor: i came prepare
Learnt a lot!
Keep posting such educational videos
स्टूडेंट की दुआ लगेगी😁
Who is here after COVID-19 ouybreak
I am! Remember to wash hands 👍
I am! I am looking to build a box to decontaminate packages before they go into the house without destroying it
SURVIVAL OUTPOST CHANNEL how do you figure? UVC rays do not pass through the ozone layer.
Seriously, can this kill viruses like covid-19 ??.
John Olson it does work, but it also kills everything else.
Using UV light to treat Covid in a person makes about as much sense as boiling them to sterilize them.
I honestly love watching bacteria colonies grow, it’s my favorite thing to watch, it’s interesting to see this though. It reminds me of my science fair project, how much bacteria does make up contain? I had a lot of fun watching the progress of the bacteria colonies and seeing them grow, it was very fun. I would like to try this one day at my schools lab, maybe with something different.
Person: *AcHoO*
*BEGONE UNHOLY SPIRITS, I HAVE GOD AND UV ON MY SIDE*
Anyone else freaking out about him not using gloves while handling the E. coli? Lmao. My microbiology teacher always made sure we handled our Petri dishes with gloves.
FightingMario54321 nope
The apocalypse: bacteria edition
Today this year it seems more like virus, but you were close my friend!
I was hoping a video like this existed. Thank you!
Thanks the video is a boon to explain community the effect of time and UV radiation on harmful ecoli found in so many water and food items we consume daily.
E.coli: *Exsist*
Creator:* THE SUN IS A DEADLY LASER*
U r criminally underrated bro
I know this may sound a bit insane, but if only we could do this same experiment but with even stronger EM waves to test how long microbes could withstand, like say, X-Ray.
A bunch of them would pass through, UV is pretty readily absorbed due to their respective photon energy levels.
Isn't it weird how he handles bacteria so normally? No gloves, no equipment, just swab like there's no tomorrow.
Nice video, thanks for sharing. Would moving the light closer to the petri dish kill the bacteria faster? Just wondering...
I am not sure, but I think it would. I think bringing it closer would make it more intense I assume.
@@sci-inspi I'm not a biologist so idk if increasing light intensity would decrease disinfection time. But, I do know that uv follows the inverse square law so the light should be far more intense the closer it is.
@@Dysfunctionality15 I think it's also depend on UV wavelength, from all I know, 260-270 nm is the best to kill E.Coli. The lower is, the less effective. I'm not sure what happend if UV wavelength is higher.
The experiment is cool enough, but you provide explaination too? Perfect.
*Casually pulls out E. Coli*
This is also why every probiotic drink is in a black bottle, so that UV light doesn't kill the bacteria.
So bacteria are mini vampires actually, themore you know.
Narration, explaining what we're looking at, and avoiding labels on wrong sides would help this a lot.
Why? You wouldn't understand anyway
Basically of reasons planets become deserts when the atmosphere is removed, constant UV bombardment.
This is an awsome channel
I have thought of an experiment involving this method. I'll cover a plastic wrap above the petri dish after inoculation where I can apply a handful of sunscreen. In theory, this should filter, or at least some, UV rays; thus having more growth compared to those completely exposed to UV. It would be nice if you have an insight to this matter.
Plastic wrap would block the UV rays. An alternative would be a agar or clear overlay with sunscreen and see if that works.
So basically bacterias are just microscopic vampires
Nah everything else is just a bigger and tougher vampire that can handle more UV.
(I liked and suscribed) so if I leave something out in the sun for 2 minutes, will all the bacteria on it die?
Not all the bacteria, but some will die.
These lights are UV-C which cannot penetrate or atmosphere.
Now I’m happier knowing my local chicken restaurant has a UV lamp prominently hanging up on the kitchen wall.
Wow, thanks for this! I will quit with overnight uv exposure. It was melting plastic tubes and Pipet tips
thanks I have 13w uv light.. I submerge it into my water dispenser for drinking.. I was wondering how long to turn on the disinfection cycles.. now I have an idea..
What was the wavelength of your UV light? Did you use uva or uvb or uvc?
UVC is the highest energy and therefore more likely to form thymine dimers, making it most lethal.
This was a very helpful video. Much appreciated. I'm sorry you had so many childish replies.
Nice work. Can you also run that using O3 generated from 185 nm and no direct UV?
This is what I was also wondering. Ozone is heavier than air so it will fall down onto the uncovered side of the petri dish. Perhaps they could seal the dishes and remove all oxygen.
so nobody is gonna talk about how this guy got e. coli sitting around in his house???
IS it in his “house” .. ?
So to protect myself from bacteria I just have to stand under an extremely intense UV light source for extended periods of time. Maybe if UV kills bacteria, it can also kill skin cancer. I think I just earned myself a noble prize.
@Dušan Xmetov And that's exactly how radiotherapy works!
Except it uses x-rays instead of UV radiation. (The former is more energetic)
UV kills every cell, high UV exposition is bad for your body.
Garlic Bread that’s kinda the whole joke
@@Sir_Budginton Yeah, since UV is literally the cause of skin cancer. Don't know how garlic didn't get it. :l
is one minute too much for the skin?-how much is too much exposure?
I love how he just handles potentially dangerous bacteria with no gloves on
Eh, the bacteria used in a lot of labs are non pathogenic, and if you use proper sterilization, handling, and clean up techniques it’s fine. You can use gloves for extra protection though (I generally do because I’m clumsy sometimes)
ive literally ate E.Coli its fine
Great experiment! Will it get the same results if use UVB or UVA?
That face when he accidentally makes a super bug that can survive UV
doubt it. Since it's destroyed at the DNA level
Your content is amazing! Can you do the same experiment with fungi instead of bacteria????
I'll add it to my list of future videos.
@@sci-inspi Thanks
I don't know why but the sentence _"Let's spread E. Coli on the Petri plate"_ Is so funny
What distance was the lamp from the petri dish? Nice experiment, thank you!!
This guy answer everyone questions even silly one am subscribing
Can you do a video on the effects of alcohol on microbes? It's kinda relevant nowadays. Thanks!
Great Video! Whic doses have you applied? 40mJ/cm2 for 30sec? and for 2 minutes? Thanks!
Hi EOS,
I don't know the dose but I could give you information about the conditions and maybe we can calculate it?
so this is is the cure to pimples - UV. thank you for this.
That wouldn't be a good idea. UV light causes sunburn. And prolonged exposure of UV after years can cause skin cancer.
UV - C are lethal
It's great for psoriasis though because it kills all the cells that are rapidly regenerating.
@@sci-inspi if im correct, it also does give a thing called arc eye or welders eye or something? witch tends to be quite painfull.
Just curious, why are you not using gloves and an inoculation loop? I'd be nervous handling bacteria that way..
How much E.Coli would i need for this for this experiment
one 5-milliliter 24 hour old broth culture of E. coli should be plenty.
bacteria: they cant kill all of us.
UV Light: Hold my beer im gonna end this shit wholes career
The real question is, where on earth did you GET E COLI??
I bought it from scientific suppliers.
its ecoli not ebola lmao
nail techs worldwide united with their uv lamps
*Grabs E. Coli with his bare hands*
No he grabbed a test tube. The E. coli is inside the test tube
the video is 3 years old, but I really liked it, I just missed seeing the PPE protection in your hands when handling the bacteria, or with your eyes in the ultraviolet light. I'm from Brazil and this video will help me do some research
hii,
you have got a good video.
would you do a video on Ozon air toward bacteria and mold pls ? there are lot of cheap ceramic ozone generator on ebay..
thanks
andrew
Let me check and see if I can get my hands on some Ozone.
great!! can't wait.. thanks
I hope you wore proper eye and skin protection when using UV-C bulbs! Even brief exposure can cause temporary damage, and anything more than a few seconds can cause burns in your eye. Apparently it feels like you have sand in your eyes all the time... some people's eyes seem to recover, others have irreversible damage. All depends on length of exposure and the power of the lamp. Best to just not take any chances. Even standard polycarbonate safety glasses cut pretty much 100% of UV-C light but even though I know it's factually true it still doesn't feel safe to me and I tend to use specialized UV blocking glasses.
For those wondering, the reason UVC is so dangerous to humans is that we are never exposed to it naturally. The sun does produce quite a bit of UVC but the atmosphere filters almost all of it out, as well as most UVB. The UVB that gets through is what causes tans/sunburns/melanomas. UVA does also play a part but it's not nearly as damaging as the shorter wavelength UV.
Does UV light have any effect on viruses, and if so, how much exposure is needed to kill them, especially hard-to-kill viruses like SARS-CoV2?
Cov-sars-2 isn't "hard to kill", just use soap or any disinfectant. The problem is, it *spreads* really easily.
@@NigelTolley Billetes, mascarillas, verduras talvez
Thank you! Now im gonna spray my piece of bread with UV light so no filth is on it!
Where do you get your bread from lol.. You don't have to do that if it's fresh bread, bacteria is killed within the oven.. But even if there is some bacteria left, and someone touches the bread, it can build your immunity to that bacteria.. If you disinfect every single food you eat, you wouldn't have immunity and you would easily get sick just by going out
can anyone tell me what is the best uv wand to get i want to make sure it's not a knock off and it kills the bacteria and i want one i can change the bulb if possible for toilet bed sheets and kitchen counter and my lazyboy. how would a person go about testing if it kills germs as it should.?
Jeannette K i believe any regular uv light will work but you can always buy some off of amazon good luck:)
it s not good to use UV light to kill bacteria in closed area like your toilet, because Uv light reacts with Oxigen O2 in the air to form ozone O3, which is toxic!
i recieved a light in the mail with no instruction it is led and it is blue it showed how what surfices needed to be cleaned I think it is a black light. Is there such thing as a led uvc light?
+Hung Tr Ozone has no smell, this thing we are feeling is nitrous oxide and this is real threat. Because ozone is hyperactive then easy oxidate nitrogen.
Those with the strongest immune systems grew up playing in the dirt, those with the weakest immune systems grew up wrapped in cotton wool.
Thank you for the explanation at the end
Madlad not wearing gloves when handling E. Coli
meanwhile in an alternate universe:
"So lets expose it in 1 min"
1 second later
"SHIT SHIT SHIT I CREATED A SENTIENT BACTERIA"
Do the same experiment with various levels of alcohol and hydrogen peroxide.
And bleach. That should be interesting.
Thanos snaps all bacterias in petri dish dishappears
No bacterias were harmed during the making of this video
Is the light here uv a, uv b, or uv c light
UVC
thanks
Now you know that summer time is the worst time for cold and flu viruses.
That's quite fast
6 likes only lmao
Not mentioned here that the UV light needs to be UV-C light. Not UV-A or UV-B. A or B will not kill bacteria or viruses. That’s a very important note not mentioned.
Would a simple 3W UV LED be enough ?
I am not sure. I used a lamp that had two 15 watt bulbs for this video. My guess would be the 3 Watt lamp would require more time to kill the bacteria. If you have access to a microbiology lab, you may be able to test the 3 watt lamp it.
what if you got a 5 gal of bucket of yeast and water that would take forever?
Sci- Inspi which wavlength in nanometers was the lamp you used?
The wavelength was 254 nanometers
Yes the wavelength is 254 nanometers. Sorry for the late reply, I never got a notification for this comment.
Very godd footage and good presentation. Subscribed!
What temperature did you incubate the bacteria in??
37 degrees Celsius
Thank you. I was researching this on science buddies and they said 37 degrees Celsius but I wasn't sure and so I asked you what you did.
You're welcome Harish.
The bulbs are LP or MP?How distant are lamps from the plate? have you irradiated the plates in a room or outdoors?
2:45
High level stuff
Good Ending: No bacteria survived
Have u ever been sick from the work u handle with ur hands. I'm cringing so hard my face is gonna get stuck like this lol. But I'm here for this
Nope, never.
So if I have a uv light on all day and night in my bathroom there will be no bacteria? 😲
theoretically, yes
The dude just fiddled with E.Coli with his bare hands lmfao
bacteriophages: AM I A JOKE TO YOU?
This would be a great thing to show in vampire movies when scientists try to figure out exactly why vampires are evolving to become immune to UV light
Is it the same light used for check the money?
Lmao maybe this is why my mother call me bacteria when she was grasping her life on the hospital then died few seconds later
Very relevant these days! Thanks!
What happens to Protozones and protozoa
Great video !! You are the best!!
Me: grows bacteria
My little brother: *Opens the samples and licks them*
Me: *TIMMY NO-*
Lol
Is it possible to drink water that has been exposed to ultraviolet rays for several hours, 6 hours, in the sun ? Thanks !
So how long would it take a light like that to disinfect someones living room?
Will it be the same effect with 290nm, UVB ?
What was the separation of the UV tube to the plate , and by 15W I assume you mean 15W of energy going in to the tubes and x% efficiency?
Cool. What's the uv bulb power?