UV effects on bacteria time-lapse
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- Опубликовано: 30 янв 2017
- Time-lapse footage of bacteria-covered petri plates growing after being exposed to UV light. UV light kills bacteria, with longer exposure times resulting in higher cell mortality.
The elapsed time is shown in each clip. The petri plates were incubated at about 37 degrees Celsius.
The lamp used has two 15 watt bulbs. The lamp has 110 volts and the wavelength is 254 nm, UV-C. 
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what if all the bacteria just ran away to the other side?
E. coli can't move on a solid medium. It would take them a very long time to move to the other side as well.
Sci- Inspi Not to mention that not all strains of E. coli are motile. Perhaps you could have used a nonmotile strain.
@@sci-inspi r/woosh
@@davidgamingremastered6784 r/notawooosh
@@davidgamingremastered6784 r/plzcommitapoptosis
What if bacteria apply sunscreen..???
It should protect them. If the chemicals in the sunscreen aren't bad for them in the first place.
If the sunscreen is zinc based, they should be fine. If the sunscreen is carbon based, the UV will just heat it up and they’ll either incubate or die trying.
Absolutely brilliant 👏👏👏👏👏👏
Let’s just hope no bacteria’s read this comment or else we’ll have a real problem on our hands.
@@sci-inspi I love how you respond to these questions that were meant to be a joke. Really shows how there are no stupid questions.
'Bacteria' is already plural
You should spread the colonies who survived into a new petri dish and repeat the process until they all survive.
That's a good way to demonstrate adaption and evolution! Thank you.
Lol
Yep
That's a good way to create a damn superbug, don't do it!
To create a new super bacteria. No, don't do that.
Germs: 'exist'
Light: im gonna end this man's whole career
The sun is a deadly laser
Did he just write them on his death note or something?
Low Quality Shitposts not anymore there's a blanket
A bacteria isn't a humanoid, if you are gonna meme do it properly. T_T
love how he answer most of the comments
I try my best
Why is there so many people talkin 'bout gloves? I work in a microbiology lab on pharma and we do not wear gloves. It' s not like splashes or something. It's all about knowing how to handle them. I believe you did it just fine. Normaly people who's afraid without knolwedge of bacterias get scared.
Larissa Amores umm! maybe because...its E-coli.
@@whendeathdeclareswar7458 Do you know anything about E. coli besides "It makes you very sick"?
You are a part of the problem Larissa describes.
@@whendeathdeclareswar7458 how you shit
if he gets e coli on his hand he could just wash it off
Yeah, in school, even when I worked with piranha solution or aqua regia I didn't really wear gloves. The only time we did was for hydrogen fluoride. If I didn't need to wear gloves for aqua regia you probably don't need to wear gloves for E. coli, especially when there's already a shit ton on your skin.
When Thanos even wipes out half of bacteria
Wayy underrated
noes noes PÉGATE UN TIRO
Would be interesting to see the comparison between this video and other types of UV light being used
Hold up bacteria! it is i THANOS
SkiLLsZ u beat me to it,
Your profile picture make it better
upvote
_"To show you the power of UV light, I covered this specimen in half!"_
Protect yourself against the might of UV light with Flex Seal!
that is a laboratory UV source, more powerful than the battery UV-C wands.
@@mango251 that funny, you know Flexseal really liquid Rubber. UV light eats and destroys elastic polymer Rubber. Rubber enemy is UV light. Don't care if it's vulcanizing like tires they degrade. That why sunscreen like a Rubber protection cream lotion. You are actually protecting the dermis, elastin elastic rubbers band's fibers in our skin, from degradation and wrinkles. Our skin, flesh matter or whatever you call it is similar to a Rubber coverage, over our body's. The pigment melanin a natural sunscreen is natural built-in our DNA. But what most don't understand is, the more skin pigmentation we have the more the skin is tighter lest ability to stretch, the less pigmentation and dna of lighter skin types the more elastin rubber like the skin is. That the way DNA ,dermis etc is , but UV will destroy Flexseal🤣😃😄😄, and Rubber and skin.
love these scientific demonstrations!
I was hoping a video like this existed. Thank you!
Thanks the video is a boon to explain community the effect of time and UV radiation on harmful ecoli found in so many water and food items we consume daily.
Excellent video! I was going to make a video showing UVC light tests using salmonella, but no need to now.
Afraid you have E. Coli on your skin!? No worries! Lay under a UV lamp for a few minutes. You'll be fine.
The experiment is cool enough, but you provide explaination too? Perfect.
How did you quantify the amount of bacteria left over?
Like is there a way to compare the growth of the control side and experimental side?
Not sure if that makes sense, but thanks
Hi Katie,
Usually you can count the number of bacterial colonies and compare the different sides. Highly concentrated cultures often leave a "lawn" of bacteria which we can see in the control side. Hope that helps.
ImageJ is also typically used so you can measure the area of patches or lawns of bacteria
You can literally see the living bacteria with the naked eye. Couldn't you see the difference between the sides? And he was just counting how many separate groups (colonies) were remaining. Just the number of colonies and total size (area, because they're on a very flat surface) should be all we care about.... not the actual number of bacterium left over.
I don't think the OP took basic biology class >.>
I honestly love watching bacteria colonies grow, it’s my favorite thing to watch, it’s interesting to see this though. It reminds me of my science fair project, how much bacteria does make up contain? I had a lot of fun watching the progress of the bacteria colonies and seeing them grow, it was very fun. I would like to try this one day at my schools lab, maybe with something different.
Coronavirus:uhhhh why i can't infected you!!!?!
Me with full uv armor: i came prepare
The apocalypse: bacteria edition
Today this year it seems more like virus, but you were close my friend!
What distance was the lamp from the petri dish? Nice experiment, thank you!!
I have thought of an experiment involving this method. I'll cover a plastic wrap above the petri dish after inoculation where I can apply a handful of sunscreen. In theory, this should filter, or at least some, UV rays; thus having more growth compared to those completely exposed to UV. It would be nice if you have an insight to this matter.
Plastic wrap would block the UV rays. An alternative would be a agar or clear overlay with sunscreen and see if that works.
*Casually pulls out E. Coli*
Thank you for the explanation at the end
Learnt a lot!
Keep posting such educational videos
स्टूडेंट की दुआ लगेगी😁
What if after being exposed the the UV light, you look down is shock as the E. coli takes of a pair of shades and says “Hey! Thanks for this nice, golden tan!”
Wait so that's not normal?
@U hahahahahahahahahhah
i saw u on the Lamborghini video too
Great experiment! Will it get the same results if use UVB or UVA?
This is brilliant. Thank you.
This guy answer everyone questions even silly one am subscribing
Wow, thanks for this! I will quit with overnight uv exposure. It was melting plastic tubes and Pipet tips
Nice! E.coli! I like to spread that on my cheese peenut butter sammich
And how long you are eating shit?
@@4ipon4ik lmfao
*you are scaring the bacteria! Shush!*
Are you sure that it's peanut butter?
@@4ipon4ik that's a r/whooosh
U r criminally underrated bro
Thanks for the video, very cool. How close were the 15w bulbs to the dish? Thanks
Nice work. Can you also run that using O3 generated from 185 nm and no direct UV?
This is what I was also wondering. Ozone is heavier than air so it will fall down onto the uncovered side of the petri dish. Perhaps they could seal the dishes and remove all oxygen.
Person: *AcHoO*
*BEGONE UNHOLY SPIRITS, I HAVE GOD AND UV ON MY SIDE*
have u tried picking up the ones that grew after exposure, spread them, and re-expose? luv ur channel, btw!
This is an awsome channel
How much E.Coli would i need for this for this experiment
one 5-milliliter 24 hour old broth culture of E. coli should be plenty.
Nice video, thanks for sharing. Would moving the light closer to the petri dish kill the bacteria faster? Just wondering...
I am not sure, but I think it would. I think bringing it closer would make it more intense I assume.
@@sci-inspi I'm not a biologist so idk if increasing light intensity would decrease disinfection time. But, I do know that uv follows the inverse square law so the light should be far more intense the closer it is.
@@Dysfunctionality15 I think it's also depend on UV wavelength, from all I know, 260-270 nm is the best to kill E.Coli. The lower is, the less effective. I'm not sure what happend if UV wavelength is higher.
Thank you so much for the information!
Great video !! You are the best!!
So bacteria are mini vampires actually, themore you know.
I know this may sound a bit insane, but if only we could do this same experiment but with even stronger EM waves to test how long microbes could withstand, like say, X-Ray.
A bunch of them would pass through, UV is pretty readily absorbed due to their respective photon energy levels.
Very relevant these days! Thanks!
Simple and nice. Thank you.
Basically of reasons planets become deserts when the atmosphere is removed, constant UV bombardment.
E.coli: *Exsist*
Creator:* THE SUN IS A DEADLY LASER*
Very godd footage and good presentation. Subscribed!
very interesting video we can learn a lot from this!
Can you do a video on the effects of alcohol on microbes? It's kinda relevant nowadays. Thanks!
Who is here after COVID-19 ouybreak
I am! Remember to wash hands 👍
I am! I am looking to build a box to decontaminate packages before they go into the house without destroying it
SURVIVAL OUTPOST CHANNEL how do you figure? UVC rays do not pass through the ozone layer.
Seriously, can this kill viruses like covid-19 ??.
John Olson it does work, but it also kills everything else.
Using UV light to treat Covid in a person makes about as much sense as boiling them to sterilize them.
Great Video!
This is also why every probiotic drink is in a black bottle, so that UV light doesn't kill the bacteria.
(I liked and suscribed) so if I leave something out in the sun for 2 minutes, will all the bacteria on it die?
Not all the bacteria, but some will die.
These lights are UV-C which cannot penetrate or atmosphere.
What was the wavelength of your UV light? Did you use uva or uvb or uvc?
UVC is the highest energy and therefore more likely to form thymine dimers, making it most lethal.
What was the separation of the UV tube to the plate , and by 15W I assume you mean 15W of energy going in to the tubes and x% efficiency?
How strong is the UV radiation? Can it be measured on an index like the sunlight’s UV radiation?
Your content is amazing! Can you do the same experiment with fungi instead of bacteria????
I'll add it to my list of future videos.
@@sci-inspi Thanks
can anyone tell me what is the best uv wand to get i want to make sure it's not a knock off and it kills the bacteria and i want one i can change the bulb if possible for toilet bed sheets and kitchen counter and my lazyboy. how would a person go about testing if it kills germs as it should.?
Jeannette K i believe any regular uv light will work but you can always buy some off of amazon good luck:)
it s not good to use UV light to kill bacteria in closed area like your toilet, because Uv light reacts with Oxigen O2 in the air to form ozone O3, which is toxic!
i recieved a light in the mail with no instruction it is led and it is blue it showed how what surfices needed to be cleaned I think it is a black light. Is there such thing as a led uvc light?
+Hung Tr Ozone has no smell, this thing we are feeling is nitrous oxide and this is real threat. Because ozone is hyperactive then easy oxidate nitrogen.
Those with the strongest immune systems grew up playing in the dirt, those with the weakest immune systems grew up wrapped in cotton wool.
the video is 3 years old, but I really liked it, I just missed seeing the PPE protection in your hands when handling the bacteria, or with your eyes in the ultraviolet light. I'm from Brazil and this video will help me do some research
I recently purchased (and am still awaiting delivery) on 2 portable LED UV-C lights and one Quartz 50W UV-C light. I want to conduct this same experiment to see if the portables work at all.They claim to be between 270-395nm, just barely within the effective range, but I need to know if they will work at all. I'm confident the UV-C table top lamp will be fine, but the portable I may want to return, even tho they're dirt cheap.
hii,
you have got a good video.
would you do a video on Ozon air toward bacteria and mold pls ? there are lot of cheap ceramic ozone generator on ebay..
thanks
andrew
Let me check and see if I can get my hands on some Ozone.
great!! can't wait.. thanks
Anyone else freaking out about him not using gloves while handling the E. coli? Lmao. My microbiology teacher always made sure we handled our Petri dishes with gloves.
FightingMario54321 nope
The bulbs are LP or MP?How distant are lamps from the plate? have you irradiated the plates in a room or outdoors?
Great Video! Whic doses have you applied? 40mJ/cm2 for 30sec? and for 2 minutes? Thanks!
Hi EOS,
I don't know the dose but I could give you information about the conditions and maybe we can calculate it?
The real question is, where on earth did you GET E COLI??
I bought it from scientific suppliers.
its ecoli not ebola lmao
so this is is the cure to pimples - UV. thank you for this.
That wouldn't be a good idea. UV light causes sunburn. And prolonged exposure of UV after years can cause skin cancer.
UV - C are lethal
It's great for psoriasis though because it kills all the cells that are rapidly regenerating.
@@sci-inspi if im correct, it also does give a thing called arc eye or welders eye or something? witch tends to be quite painfull.
Is it possible to drink water that has been exposed to ultraviolet rays for several hours, 6 hours, in the sun ? Thanks !
It would be very helpful to know what distance the light was from the E. coli plate
Does UV light have any effect on viruses, and if so, how much exposure is needed to kill them, especially hard-to-kill viruses like SARS-CoV2?
Cov-sars-2 isn't "hard to kill", just use soap or any disinfectant. The problem is, it *spreads* really easily.
@@NigelTolley Billetes, mascarillas, verduras talvez
Is the light here uv a, uv b, or uv c light
UVC
thanks
thanks I have 13w uv light.. I submerge it into my water dispenser for drinking.. I was wondering how long to turn on the disinfection cycles.. now I have an idea..
Are 70w UVC lamps for hvac systems effective in killing bacteria and mold or is the air moving by too quick to have any effect?
Do the same experiment with various levels of alcohol and hydrogen peroxide.
And bleach. That should be interesting.
So to protect myself from bacteria I just have to stand under an extremely intense UV light source for extended periods of time. Maybe if UV kills bacteria, it can also kill skin cancer. I think I just earned myself a noble prize.
@Dušan Xmetov And that's exactly how radiotherapy works!
Except it uses x-rays instead of UV radiation. (The former is more energetic)
UV kills every cell, high UV exposition is bad for your body.
Garlic Bread that’s kinda the whole joke
@@Sir_Budginton Yeah, since UV is literally the cause of skin cancer. Don't know how garlic didn't get it. :l
is one minute too much for the skin?-how much is too much exposure?
THANK YOU!
Is there particular brand of UV lamp?
What are the specs?
Been thinking of using UV light to kill mold on peanuts.
Also, is there a national or international organization with information on UV lighting and its uses?
Thanks
*Grabs E. Coli with his bare hands*
No he grabbed a test tube. The E. coli is inside the test tube
What temperature did you incubate the bacteria in??
37 degrees Celsius
Thank you. I was researching this on science buddies and they said 37 degrees Celsius but I wasn't sure and so I asked you what you did.
You're welcome Harish.
Could you list the item and where you bought them please, I would like to duplicate this experiment
Very Nice!
Madlad not wearing gloves when handling E. Coli
Would a simple 3W UV LED be enough ?
I am not sure. I used a lamp that had two 15 watt bulbs for this video. My guess would be the 3 Watt lamp would require more time to kill the bacteria. If you have access to a microbiology lab, you may be able to test the 3 watt lamp it.
what if you got a 5 gal of bucket of yeast and water that would take forever?
Sci- Inspi which wavlength in nanometers was the lamp you used?
The wavelength was 254 nanometers
Yes the wavelength is 254 nanometers. Sorry for the late reply, I never got a notification for this comment.
Will it be the same effect with 290nm, UVB ?
Really love your music :)
Thank you!
That's quite fast
6 likes only lmao
2:45
High level stuff
Nice explained
Thank you
So how long would it take a light like that to disinfect someones living room?
I love how he just handles potentially dangerous bacteria with no gloves on
Eh, the bacteria used in a lot of labs are non pathogenic, and if you use proper sterilization, handling, and clean up techniques it’s fine. You can use gloves for extra protection though (I generally do because I’m clumsy sometimes)
ive literally ate E.Coli its fine
Good video. Thanks
Thank you
How long of an exposure dows it take a virus to die?
Also what kind of UV light is used for the purpose of disinfecting?
Thank you!
distance to the light may also play an important factor in addition to the length of exposure.
a highly educating video clip with interesting comments
How effective would it be to treat scrapes and cuts with UV light as a method of sterilizing a wound?
i'd like to do the same experiment to test the power of one of my sanitizing UV light.
what harmless bacteria could i use to see a result with naked eye like that? e.coli seems dangerous, kinda. maybe just the germs on my hand?
and what solution should i use for them to grow in 24h? water+sugar maybe? when you're applying on the petri dish, it seems kinda watery, i guess there's a solution with it.
thanks
I have a question, if anyone knows I would appreciate an answer please.
I am about to buy a UV-C and Ozone wand/stick to sanitize things at home and car, and I see that there are lots of different Watts, but I don’t understand why, is it that the smaller the watts the less effective they are or that it takes a longer time to kill bacteria and viruses or what?
I see wands from 2.5w up to like 11.
Thanks a lot guys!
Is there a way I can do a similar test, but not E. coli? Preferably something that I can safely obtain. I have my own UV sanitizer light, but I don’t know if it works properly.
They've been using that system for years in the water purification plants
So does the explanation at the end apply to sunburns on our skin as well. Eventually the skin flaking off later?