Clearing and Staining Fishes
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- Опубликовано: 25 июл 2024
- Diaphonization -- otherwise known as clearing and staining -- is one of the most photogenic preparation methods used by research scientists. It's beautiful, but is it practical? We interviewed Dr. Caleb McMahan, Collection Manager of Fishes, on how he uses this technique to answer questions about the evolution of fish!
To learn more about what's happening in Fishes at The Field Museum, check out their webpage: www.fieldmuseum.org/science/r...
Big thanks to Caleb for taking the time to share his work with us, and to Alan Resetar for lending the reptile and amphibian specimens!... as well as the extra light table because ours broke right before filming. :(
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Producer, Writer, Creator, Host:
Emily Graslie
Producer, Editor, Camera, Graphics:
Brandon Brungard
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Filmed on Location and Supported by:
The Field Museum in Chicago, IL
(www.fieldmuseum.org)
I would love to see the clear and stain process performed on video.
+DaGizmoGuy We debated showing the process, but to be honest it isn't that exciting as it takes a while between putting the specimen in the various solutions and letting them sit for days without a whole lot visibly happening. Perhaps sometime soon we'll set up a timelapse in the lab and try to capture the transformation that way!
+thebrainscoop Sooner rather than later, please, on that timelapse!
+thebrainscoop Seconded, I want to see that time lapse too!
+thebrainscoop Yessss timelapse!
+DaGizmoGuy A timelapse would be absolutely fantastic and well appreciated if you guys can :)
I am amazed by how much research is being done at the Field Museum.
+ljmasternoob I'd say it's typical of all museums
666Tomato666
But at this level? Over 140 scientists is a lot.
+ljmasternoob Big museums.
+ljmasternoob Have you been there? It's a damn huge museum. I mean, Emily has shown us some parts of the museum on video, but until you've gone there in person, it's hard to understand how big it really is. I'm surprised it's ONLY 140.
ShadeSlayer1911
It is on my list of science pilgrimages I need to make.
I would love to see a timelapse of making these clearing and staining fishes (or something else). Just to see how everything changes. Awesome vid btw I now know how they do it!
I found this channel for the first time yesterday. I instantly subscribed and I can not stop watching.
+JakeBobProductions One of us! One of us!
+Mr. G. Ruff one of us, one of us
+Mr. G. Ruff we accept him one of us
Squirrel McNastyface
+Mr. G. Ruff Gooble Gobble! Gooble Gobble!
i could stare at those images for hours. damn, the colors are amazing :D
"Check the gonad jar jimmy" Oh Lord xD
right?....sometimes I feel like my ex wife still has my gonads in a jar
+William Scott Patterson Oh...Well I'm sorry
+William Scott Patterson You had a bad divorce lawyer.
Boy did I ever
Couples who stain together stay together
+Cindayyee it's only a little awkward when she looks directly into the eyes of her partner telling him to put the gonads in the jar.
I've waited for you to do this episode for so long and now it's finally here! Thank you so much, Emily, it was beautiful.
I don't know, I think it'd probably be worth doing this to a human to see what we can learn about our own bodies. Depending on how expensive it is it might be best to leave that to an eccentric billionaire instead of draining public funds, but isn't doing weird things with their corpses what eccentric billionaires are for?
Tacokel does it lol
Pretty sure the Body’s exhibit has parts that are clear
i always love your videos, but i've gotta say, this episode was shot particularly well -- and the audio was on point.
I'm not sure how useful a cleared and stained Emily specimen would be. I mean, even after the whole process I'm pretty sure we'd see that it's still got brains on it...
That little skate/ray looks AMAZING! What a great job!
Those specimens look like some form of art.
Woah there Jimmy, those fish bits are for science only.
This video was great! The audio, framing of shots and chapter videos/images. Great, great, just great.
I love stainings like these, they are the perfect combination of science and art!
He was great! Great video.. love hearing people talk about their passions, and he made it very clear :)
"I'm a mammal Greg, can you clear and stain me?"
AHHH I WAS THINKING THE SAME THING!!! I am glad I wasn't the only one to want to clear and stain a body.
IT MUST BE DONE
+ellock1998 They've done it with fetuses. Just ask the internet
Also check the search phrase "diaphonized fetus". If you are not creeped out by childrens skeletons.
THAT IS AWESOME! I LOVE IT (In a purely scientific way... of course)! Thanks for that. I didn't know it was called diaphonization (?). These are so cool.
You're welcome :)
+ellock1998 that is the kind of thing that would make a nice funeral
M , another great video and you do such a wonderful job at doing it. I always enjoy your videos. Thanks
This method is *so* awesome!
I am now going to look for videos, where I can see how the actual process is performed.
Yet another amazingly interesting behind-the-scenes episode, Emily ^^ Diaphonization... another word I had never heard before. Seems to be pretty useful. And it looks pretty cool, too.
I've always wanted to know about Diaphonization. Thank you BrainScoop!
I swear, that jingle makes me so happy! Ting ting ta ting ting...I light up every time I hear it.
Fascinating and extremely useful
YAY more episodes!!!
Brain scoop makes the bleary autumn far more fun!
It is so cool that The Field Museum has so many cool fish and amphibian specimens.
VERY COOL! I really enjoyed this episode, I'd only every seen these in passing but it was cool to understand the process :D And they look very awesome.
I didn't realize how cool this would be!
i’m so glad i found the brain scoop again this was always one of my favorite channels
I love this channel so much
Love watching your videos.
I love the ray! These are all beautiful, but that one looks like something you could hang on your wall! ^.^
I've gotta try that as a pick up line. "That would take a lot of trypsin, ladies." Thank you for the awesome episode! you guys always rock.
Great vid again!
Beautiful! What artwork that would make. But omg the disappearing and reappearing fish! What magic is this! LOL
This was truly fascinating
WHAAAT. WOAH! This was just so amazingly cool! I had no idea clearing and staining animals like this was a thing until watching this video. Now I really want to see one in person.
Fantastic! This is great example of the vast riches found on RUclipss video servers - the channel "for the people". Many thanks for your video. I just had to chime in and say, "COOL!".
They are so beautiful and useful! Cool!
Man, i would love to have a job like Emily! She gets to dip into all types of sectors of science (and look at some of the coolest things on earth) and spread knowledge that a lot of people would never get to see!
That was really cool! Throughout the video, I was wondering too what it would be like to do this to a human.
I think it's interesting just how informative this actually is, being able to move around the skeleton and joints, you could learn a lot by that.
Hypsophrys nicaraguensis! I had this fish in my aquarium for years. In the aquarium hobby it's known as the "Nicky."
Mr Emily is super cool. Great video guys
Nice video! keep up the good work (a.k.a my favorite one this channel)
I'm pretty sure I saw those colours used in the Bob Ross stream.
"Lets take some fish-bone-violet; just a little, yeah a little from the fish-bone-violet."
+Sardinfangfischnetz ! "And lets use that fish-bone-violet to have some happy little skeletons right here on the light box."
Informative and pretty
Awesome process! I found some of these teeny tiny stained specimens in 2 inch jars for sale when I visited Tokyo! I regret not buying one of them now :(
This is my new favorite channel of forever!!!!! *squee*
That ray looks so amazing, it's beautiful!
Edit: also that pelvic region on that frog looks so weird :D, cool
My internship had a really cool video conference with some internso at the Smithsonian and they had a puppy that was stained like this. It's such a cool way to look at a specimen. Woo brain scoop^^
I always wondered how this was done! Woo! :D
THIS IS SO COOL!
this looks so artsy
"Check the Gonad Jar" is my Jimmy Eats World cover band name
THIS IS SOOOOO COOOOL
I was really enjoying this, and then she asked if he could clear and stain her - I died laughing!
I love the editing done! Kudos to Brandon Brungard I see
fascinating
A cleared and stained Emily would be the coolest.
That's so cool to see inside a fish or reptiles and the bones
how beautiful!
Super cool.
amazing.
Yay! Cool things!
that is one of the coolest things i have ever seen. not only is it a fascinating process, it also looks very aesthetically pleasing for some reason.
Is this something a layman could do as well, or are these chemicals restricted in their availability outside of professional scientific research? Also, could you encase these specimens in something like clear urethane resin or would that not work due to their gelatinous surface?
I just want a tour of one of the stained specimen like that little ray.
Can we just get Dr. Caleb just explaining the morphology of the ray? That will be so cool
Oooh i would love to see this process done to larger animals!
Nice to see Raccoon keeping his/her skills up-to-date :D
Holy poop this looks awesome o.O
Huh. Very cool to know about.
Funny to see a technique I use in my lab shown on here. We study zebrafish fin regeneration and we use this staining to look at how fins regenerate.
That so cool.
Are there any simple guides out there for assembling fish skeletons? Because I have a few and it's impossible to keep track of all the parts, especially around the skull.
This is so cool! And now I want those fishes on my walls... Hmm, what would happen if you encased such a specimen in like clear resin? Would it rot? Would that have a different refractive density and would that make it cloudy? I mean, it would probably be easier to paint one to put on the wall, but the real thing would be so cool (especially if you put a light behind it)!
alizarin crimson (red) is the color red paint Bob Ross uses in the first season of "The Joy of Painting" neato!
That first word above should be spelled "diaphAnization" (process of making diaphanous or transparent). Nice video!
Emily and I asked the exact same question at the exact same time about clearing and staining ourselves.
THIS IS THE COOLEST THING EVER!
Is it possible to buy these to put on my wall?? It's so interesting and beautiful!
Not just biology today but chemistry too. :D
:D I've always wanted to know how this was done. Now I know, it's actual magic.
They had some fetuses at body worlds nyc that were prepared in this way! It was a very morbid room but it was amazing to see the tiny skeletons in fetuses that were only a few weeks old.
I was wondering if there is a published protocol for this method somewhere online?
+Erika Martin This paper from Taylor and Van Dyke (1985) seems to be one researchers reference as a good baseline, but as Caleb mentions in the video there are a few different ways to go about it depending on the size of the specimen, how old it is (e.g. he has cleared/stained specimens collected in the early 1900s that have sat in alcohol for a century), etc. But, you could start here: sfi.mnhn.fr/cybium/numeros/1985/92/01-Taylor[92]107-119.pdf
That's so cool :-D
You should start a kickstarter for getting cleared and stained now! That way you'll have enough!
How long does each stage take generally? Because these are being absorbed by static (dead) things I feel that each stage would take a considerable amount of time. Plus what looked like a small ray of some description looked great, can we get a close up of that . It kinda reminded me of the old screen-savers of dancing lines.
I've always thought of doing that but I don't know where to get the supplies. So I just preserve them.
Can you stain the bones other color for example green?
Would the process change if I wanted to do that with bats?
Thanks!
damn this looks cool
It's beautiful.
Is it possible to preserve a specimen in resin and still be able to see them clearly?
Is there shrinkage?
The stingray(-ish?) and turtles look amazing. Couple of questions:
- Can mammals be cleared too? He only mentions "other vertebrate groups"
- Can you reintroduce slight color variations in the soft tissue? It would be amazing to be able to distinguish muscle and such on a translucent level while still be able to see the bones
+Carlos Vives you can buy cleared and stained rats on Amazon, and there are several ways you can do it at home. I'm not sure if you can stain muscles, but you might be able to still see them if you don't completely clear the specimen.
Joseph Colley
Heh, it's true you can find anything on Amazon these days :) Thanks for the information
Why not just put them in oil or smoke them? Much tastier afterwards.
+Pelle Olsson Well, glycerine tastes sweet and is classified as non-toxic, so I guess you could think of the specimens as candied fish?
Edit: Now, purely out of curiosity, I wonder if a person could actually eat the specimens. Aside from the fact that there's probably no nutritional value and that it'd be like eating bones, plus it would destroy something with scientific value but...could it be done? I'm guessing yes.
E Hernandez Well, a human can eat practically anything. Some things but only once... *cheesy horror laugh*
Pelle Olsson Good point! Now, would you like to try my arsenic cookies? They are to die for!
E Hernandez I always liked my sweets bitter.
+E “Anonymous Nerdfighter” Hernandez Dibs on the eyes
I went to the same place Emily did immediately, where I wondered if I could be cleared and stained.
I wish I lived at The Field Museum.
New Kickstarter Campaign! Clear and Stain Emily.
i hope you can digitize those specimen with 3d scanner. its easier to shared and distributed that way
+Eko ari wibowo as a visual reference, sure. I loved it when he manipulated that one fish to show the articulation of the bone structure
kazoosc that will be great if anyone can make 3d model with manipulated joint
+Eko ari wibowo this is a cool idea. One issue I can see with it would be that typical small scale 3D scanners (which often use a form of triangulation) would have trouble modelling the refracted path of the light through the transparent collagen/glycerine (they have the same refractive indices as one another but not air) in order to extract a clear structure of the innards. I don't know that it would prevent it from working, but it would make it more complicated for sure :)