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How to turn any light microscope into a dark field scope
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- Опубликовано: 1 июн 2014
- For less than $1 US, convert nearly any light microscope into a dark field microscope. Commercial conversion kits can cost upwards of $100. This cheap, easy, fully reversible technique allows even the most basic 'scopes to be upgraded.
(c) 2014 Ambithusia
Dark field always strikes me because of the beautiful colors. Thanks for sharing!
You're the Alton Brown of microscopy.
Well done, thank you.
More, please.
After watching this and doing a little more searching for ideas, I 3d printed a range of sized discs to fit in to my filter holder, which is at the same place you placed your blackout sheet you made, works good to about 0.65 Objectives (4x 10x 40x).
One thing I noticed on my microscope was I had to raise the condenser to sit a few MM under the slide to get the dark-field to work.
Big thanks for sharing this info as you saved me £80-£100. ; )
Thank you very much Dan! I was considering a dark field, but the price was too high, so having this ability to make a lightfield into a darkfield is much appreciated.
Gave a thumbs up just for the 'something somthing' bit of the vid lol. Thank you for the advice, I'm going to try dark field now!
Thanks! Suggest simplifying by cutting the opaque spots out of black construction paper, instead of using a Sharpie marker and stacking. You can just put a dab of adhesive on the construction paper dot to attach it to the clear plastic.
Thank you! If you will pardon the pun, I found your presentation "illuminating". I just got the wrong type of microscope for what I'd like to do and you gave me so much inspiration. I'll follow and subscribe so my thanks becomes a tangible thing. Carry on!
We need more people like you on RUclips, thanks! :)
Check out microbehunter
@@WillyDrengen thanks, I just discovered his channel :)
@@Seyhan60 No problem, Microbehunter is just such a great guy.
just ordered a microscope today and I was looking for ways to view darkfield on it ( quite cheap, no commercial adapter available) and with your amazing solution, it's brilliant! thanks man!
I have this strong urge to come down and help you maintain precision!
Thoroughly enjoyed the content, great work, and I got to learn something!
Very good idea! Thank you so much for sharing this with us ! I am going to make my light compound microscope dark field now :D
here this one can be done also easyand cheap, just 2 polarising filters , 1 for the lightsource and 1 for the Eyepiece (CPL Filters can be found from 3$ each in the analog cameras section on ebay for example on ebay in your country or on ebay china)
that's not darkfield, it's phase contrast you're appearing to be doing here.
That’s neither darkfield nor phase contrast but...wait for it...polarized light aka polarization contrast
Thanks for this useful Video. It confirmed by suspicion that darkfield is a very simple modification.
Thank you; this I will try. I accidentally partially blocked light from the condenser with a small ruler and ended up with a partial dark field. It helped with the contrast.
"Something something something dark field..."
Just waking up, thank you for the first laugh of the day! Better than a cup of coffee.
Excelent and funny video. I just buy a microscope and I am eager to convert it in a dark field microscope. I will try these great idea. Thank you.
very nice, as an amateur I appreciate your video, keep up the good work!
Well I'll tell ya that was just about the most useful vid I've seen on youtube in a long while, super job on that, I'll be scoping out plastic sheets pronto! Thx
Thank you very much for the video tutorial. I was just needing that for my microscope. You're great!
Thanks, easy and simple.
Keep the good work
Excellent tutorial and explanation, Bravo!
Very very useful! Well done!! Thank you very much :)
I make em from plastic bottle caps. Very rigid, no cost and easy to work with
I love your midnight movie voice! LoL!!
Thank you very much! I'm looking for buying new one darkfield condenser but now I am trying to create it.
olique illumination is better than dark field. Simply use LEDs putting them a bit aside on filterholder
I have done that at low magnification. Now I have the cash for the real thing. Hope I am not disappointed with it.
Hi, thanks for the video. I found it very informative and helpful to get more use from my microscope.
Very clever... Now please show us how to update it into a phase contrast xD
Niceeee! This is a killer microscope 'hack'. Thanks!
Excellent work Dan!
thats just awesome. thank you for this tutorial :-)
are you going to make some more videos ? you are good at it
Sir you are a wizard
thanks, i enjoyed your video, it was informative and concise
great tutorial, thank you so much!!
Thanks!! I was researching here, it's not that expensive slide sheets to print on a laser printer (fortunatly I already have such a printer). I'll try printing some!
Fantastic, thank you!
soooooo good thank you 🙏
Thanks. That´s only works in 5x and 10x. Someone know how to make that in 40x or 100x? I discovery a very easy way to do that , with 5x and 10x, with a specific kind of filter suporter: open the diaphragm and move the filter suporter to the right until you get the image. Will work too.
I have the same problem with 40x
Thanks
Great vid! Good presentation, simple, inexpensive, effective! Tnx!!! rc
Works well after some time spent with fitting. I made one a couple years ago.
How about polarizing for the DIY person?
Thanks Dan!!
brilliant. you saved me a few hundred dollars. thanks.
The field can be darkened further by enlarging the shaded diameter.
Can we use a similar technique for solar filters on telescopes?
I really enjoyed this.
I wonder if you could just paint the coin you use black and glue it to the transparent material.
Thanks for the tip!! When will you be producing another video?
My thumbs up came at the something something something part😂😂😂
Awesome. Best tutorial ever!
Great, for the beginner to understand the case - super!!! What about polarization and DIC? I just bought a microscope and all that extra stuff is so expensive and i have no idea how to apply it :-) i am gratefull for help.
Hi Dan!
What kind of microscope would you recommend a beginner that wants to view red blood cells under darkfield?
Thanks for this DIY!
This is my question as well. Did you ever find the answer?
great work .
Much appreciated top job. Thankyou.
Terrific, thanks!
So it’s not true or true because of strength that only Arizona & Nevada allow dark field microscopes
Excellent ! Thank you so much :)
Thanks! It worked very well!
What would be a good low cost scope for viewing live blood?
can't you just colour a paper circle in black and cover it in plastic circles
You are a good speaker
Nice information, do you have any trick for inverted microscope to turn them in phase contrast such easier way?
Oh, thank you very much for that cool idea =)
Thanks sir!
Genius! I’ll use it thx
This might be a silly question but could you do something like this with a flashlight and jewlers loup. By covering the light from the flashlight with the black plastic and looking thru the loup? I know nothing about microscopes! I still have a lot of learning to do.
I've been looking at gems as a hobby. I stare at them for hours with different magnification loups and a flashlight. I keep hearing about "dark field illumination" when I watch gem identification videos on RUclips. Until I can get my own microscope I thought maybe this might be a very rough diy way!
Thanks for the video... now when I do get a microscope I'll know how make my own dark field :) Plus it was funny! Nicely done!
Thankyou
Nice, i need help or direction. I want to see water clusters. Is thier a way.
I tried this method to look at the spirochetes in my blood, and it worked well at 10x. But when I try it at 100x with immersion oil it doesn't work. The image shows up faded and looking like a poor lit version of a light field microscope. Do have any ideas why this would be, and how I can make this work for high power with immersion oil?
Phillycheesecreampie Cake u need a smaller cirkel for bigger magnifications
Phillycheesecreampie Cake no just kidding u cant get darkfield with high aperture objectives
Uhm... Why are there spirochetes in your blood?
Did you get it managed to see good pictures of the blood in 100x?
Excellent
THANK YOU!!! I FINALLY FOUND SOMETHING ON MAKING A DARK FIELD FILTER!!! **Explodes.**
Nice video bro.
How do I know when I have the right diameter for the light block? Thx.
Thank you so much for your vid. Please give me some advice, I am growing some lactobacillus cultures (now trying to replicate some L. Reuteri). I would like to be able to see the action and make videoclips and pictures. Would an Omax 40-2500x work, is that enough amplification??? Good luck!!
thank u ...so help ful msg ...keep it up ..
How do you film the microscope? do you have a usb lens or you put the camera in front of the eyepiece?
❤❤❤ ⚘️🌷⚘️. .Thank You Verry Much..🙏🙏😊😊😊👍👍👍
Brightfield
Nice video ! Nice person.
Great idea, thanks for sharing. Wouldn’t it be quicker and easier to glue something opaque onto a single plastic sheet instead of colouring several sheets? Or am I missing something?
I was wondering this as well.
Yes that would work just as well - opaque disc should be as thin as possible
I gotta look at some mites from the dog
I love you.
Meet Dan, He is a BAMF!!
omg we need this :D
That guy used is Brain 🧠= 110% 😅
Somthing Somthing Somthing...not cheap...Ha!!! You can say that about so many things today...
Asewome!!!
I think 7 don´t like this Idea becauce they are selling Darkfield Microscopes .
stop waiving your hands in the face of the viewer--it;s offensive
What is with your odd enunciations ?
Moronic bordering on bonafide lunacy
I am here from the future to tell you to buy Dogecoin.... you'll tank me later
Very very useful! Well done!! Thank you very much :)