🔬 Upgrading a microscope to darkfield | Amateur Microscopy

Поделиться
HTML-код
  • Опубликовано: 28 дек 2024

Комментарии • 68

  • @danev1969
    @danev1969 4 года назад +21

    Your recommendation of the Swift SW380T was good timing. I upgraded to this microscope and find it excellent for my photography goals. I will try the dark field, thanks for that too. For those of your viewers who have this model, I have a measurement that will be critical when using the trinocular camera mount with their DSLR or mirrorless camera. When I called customer Support in Texas, they could not tell me the focus distance the camera’s sensor needs to be.
    If you measure the distance from the base of the black camera mount (where it meets the blue-ish base, to your camera’s sensor, it should be close to 90mm +/- 10mm. What this means is that if you are using a deep t-mount adaptor, your camera may be too high. I am using a M4/3 Olympus and I had to order a thinner T-mount adaptor. Once you get you camera lower down, the imaging should be great.

  • @MemoriesRR
    @MemoriesRR 4 года назад +9

    I have been using dark field for a while but I sill learned from this video. Outstanding.

  • @wackyvorlon
    @wackyvorlon 4 года назад +12

    Oh my GOD. That is incredible! I need to tweak it, I think I’ve got the black part off centre, but it is absolutely astounding. Thank you so much for this! I have the SW380B, and I can’t believe an instrument I own is capable of this!

  • @matgggg55
    @matgggg55 4 года назад +6

    I made and used oblique illumination to look at a jelly fish I found on the beach it looked AMAZING!! I wish I could share pics/video in the comments I only have the 380B swift but it looks professional! Deff take this guys advice he has never let me down!

  • @Mythreesons137.
    @Mythreesons137. 3 года назад +2

    Dark field condenser is the way to go.

  • @AM-pp5rl
    @AM-pp5rl 4 года назад +6

    Awesome explanation, however my condenser does not have a filter holder ( abbe 1970) is it possible to have the same effect if you place the stop patch on top of the iris?

    • @dylanschulz9118
      @dylanschulz9118 5 месяцев назад

      It should be possible, but will definitely require a different size. What you are doing is getting rid of the light pathway that directly hits the microscope objective but leaving the light pathway that would go around. So no light enters the objective directly from the light source, only if it is scattered by something - i.e. your sample

  • @disc4653
    @disc4653 4 года назад +2

    Do you know how to make a diy specimen slide?

  • @sonidophore9100
    @sonidophore9100 4 года назад +2

    Thank you! I was actually really wondering about this. Gonna go try.

  • @attackemartin
    @attackemartin 4 года назад +2

    Thank you for the video! Could you share the stl files for these darkfield filters?

  • @colsanjaybajpai5747
    @colsanjaybajpai5747 Год назад

    The diameter of the center black disk will depend upon the aperture of the objective. One cannot use one size disk for all objectives

  • @aardvarker
    @aardvarker 4 года назад +4

    I have the exact same microscope as you from video #6 (the educational one in the middle). It does have the ability to add darkfield, and I 3d printed many shapes to try out, and it works well at low resolutions, but I can't get it to work with the 40x objective. Did you try your filter on this microscope? I'm wondering if I'm missing any critical adjustment (e.g. open the diaphragm, lower the condenser, etc). Any tips are highly appreciated! :-)

    • @gabrielyosei7565
      @gabrielyosei7565 4 года назад +2

      I have the same microscope and can't get it to work at 40x either
      I have been trying for some time

    • @roelmattheeussen1117
      @roelmattheeussen1117 4 года назад

      good i found this comment. I have the SW380T and have excellent results at 4x and 10x, but no luck at 40x - have all different filter sizes but no difference. Is your condensor perfectly centered? On the Swift there are no centering screws.

  • @jetjazz05
    @jetjazz05 Год назад

    Thanks for the video, I just made one but using a piece of electrical tape for the black dot, worked like a charm! It really is quite nice for 40x magnification.

  • @haydenbeames5585
    @haydenbeames5585 2 месяца назад

    If you are looking at something non-transparent, such as a computer chip, does this method of imitating DIC still work?

  • @UNDERST0RY
    @UNDERST0RY 2 года назад +1

    It's very beautiful. Is it not possible merely to tape a coin or thin washer onto a cheap blue filter or rigid plastic cutout?

  • @LostBeetle
    @LostBeetle 6 месяцев назад

    I used the clear plastic and paper method, I am perplexed. 4x and 10x objectives I get dark field, if I use 40x or 60x it looks like bright field with the light turned down too dim. I have tried sizes ranging from 15mm all the way to 24mm. I can get oblique illumination to work beautifully, which looks amazing I have to say.

  • @elenaacim
    @elenaacim 3 года назад

    Thank you! I learn something new every single video. 😊
    🌿

  • @daideno
    @daideno 2 года назад +1

    this is great, but i have a problem. darkfield only works using the 15x eyepiece + 5x Objective ( w a L101 that has 5X, 10X, 45X(s) +5, 10X, 15X. Eyepieces: total 675X,) followed your exact specs. making the dark circle bigger does not work... is it the mirror ilumination? ( using ext ligth source) sorry 4 the bad english!

  • @Metalsenpai
    @Metalsenpai 4 года назад

    You can move the place where you put the filter, like in 05:24, turn on the light and make some adjustments until you see a shadow in the slide. Will work like a filter. Somebody know how to make this tecnique work in high magnifications?

    • @gabrielyosei7565
      @gabrielyosei7565 4 года назад +1

      Only 4x and 10x
      Ican't set 40x in dark field

  • @MegaPugsley1
    @MegaPugsley1 3 месяца назад

    What if your microscope does not have a filter holder? Mine is low cost #150 type with a rotating ring of different size apertures.

    • @MicrobehunterMicroscopy
      @MicrobehunterMicroscopy  3 месяца назад

      I know these. The apertures are too large anyway to make a difference. In this case you can not do darkfield. You can attach other filters there (polarization, color etc). or a cut-up credit card (etc) with a larger pin hole. The aperture is then very small (dark) but the depth of field goes up greatly. Off-center pinhole might give you oblique, but not sure.

  • @wackyvorlon
    @wackyvorlon 4 года назад +2

    I was wondering, what is the movable condenser on the SW380B for? When might you want to move it?

  • @roelmattheeussen1117
    @roelmattheeussen1117 4 года назад +2

    hi, do you have any suggestion on how to enable dark field at 400x magnification? I have the swift SW380T, but somehow i cant get it to work. Best filter size for mine is actually 1,3 mm - 1,5 was a little too large (gave a dark brown blob in the center). Many thanks!

    • @MicrobehunterMicroscopy
      @MicrobehunterMicroscopy  4 года назад +2

      The filter size should be much larger, about 15mm for the 40x objective (it must be even larger). Watch this video: ruclips.net/video/6hh5ZyU35yc/видео.html

    • @roelmattheeussen1117
      @roelmattheeussen1117 4 года назад +1

      @@MicrobehunterMicroscopy thanks! Did you get it to work for the 40x objective? It doesnt seem the condensor size/lens combination allows for it

    • @legoworks-cg5hk
      @legoworks-cg5hk 2 года назад +1

      @@roelmattheeussen1117 switch to 10x objective and move the condenser until it appears brightest and then the switch back to 40x

  • @krishnakantanayak7667
    @krishnakantanayak7667 4 года назад +1

    Sir plz send a video on cleaning lens

  • @NengVang2007
    @NengVang2007 4 года назад +1

    After watching your videos I just bought this swift 380t from Amazon and I looking to 3d print a filter like the one u got any link to download the STL?

    • @federicacapace3318
      @federicacapace3318 3 года назад +2

      ruclips.net/video/UgT52XoQGQQ/видео.html
      you can find the the download links in the description of this video :)

  • @bobbysnickers249
    @bobbysnickers249 Год назад

    Do you think doing darkfield this way would work for live blood analysis?

  • @PeaceNinja007
    @PeaceNinja007 Год назад

    Is darkfield the same as grayfield?

  • @ralfschooneveld3186
    @ralfschooneveld3186 Год назад

    I have a Euromex BioBlue Lab, with halogen light. Anythingh I can try to mimic darkdfield? Or is it only the blue filter?

    • @ralfschooneveld3186
      @ralfschooneveld3186 Год назад

      Oh, wait. 3 W NeoLEDTM illumination . Cool I can use this. Many thanks for sharing. I will try.

  • @rustynuts4426
    @rustynuts4426 3 года назад

    Can this microscope be converted to phased contrast?

  • @jo_crespo11235
    @jo_crespo11235 Год назад

    Awesome! I have a SW380T but can't achieve this results. I tried to manually make darkfield filters but they are far from perfect. Did you change the lighting?

  • @rcpd33
    @rcpd33 3 года назад

    Would it be at all possible to add a or make a dark field filter for the telmu microscope or no ? If so how would I do that?

  • @td6935
    @td6935 4 года назад +2

    Can you send me a link to a proper darkfield filter for a swift sw380t ? (on german amazonsite would be optimal

  • @pishyenaz
    @pishyenaz Год назад

    Thanks for info I really like your channel have a request can you review the vabiooth microscopes?

  • @energiavadasz3328
    @energiavadasz3328 4 года назад

    WoW, that is a great info. Thank you, Can you help me what kind of Microscope and techniks do i hav eto use for viewing parazites in my blood. In my case i am a borrelia infected. Lyme sick.

  • @Sundrop_and_friends_
    @Sundrop_and_friends_ 2 года назад

    Hi! Can you recommend a good microscope to get to try this? Not too expensive....

  • @agapeguy777
    @agapeguy777 Год назад

    That is way too cool. I have an Amscope T490B-DK scope and most recently purchased their optional non-turret version of a PC (Phase Contrast) Kit for $300 to convert to and experiment with that lighting technique. Now I have both darkfield/PC lighting options to work with! 👍

  • @Aledostoevskij
    @Aledostoevskij 4 года назад

    Thank you !

  • @3ittybittypiggiesstorytime547
    @3ittybittypiggiesstorytime547 3 года назад

    Great video! Thanks!

  • @alhaeri1
    @alhaeri1 3 года назад

    thanks

  • @hannahcampbell5880
    @hannahcampbell5880 Год назад

    Thank you king!!!!!!

  • @rahulkumawat8606
    @rahulkumawat8606 4 года назад

    Hi Oliver this item is not available in India so please suggest a different microscope

    • @matgggg55
      @matgggg55 4 года назад

      Swift , Omax , and amscope are all basically the same in terms of quality hope u can find one of those brands for sale

    • @rahulkumawat8606
      @rahulkumawat8606 4 года назад +1

      @@matgggg55 thanks bro

  • @ahmetkaracaoglu5617
    @ahmetkaracaoglu5617 Год назад

    Awesome.Thanks...

  • @kadirdalmis4321
    @kadirdalmis4321 3 года назад

    Super İdea . Thank you .

  • @esphilee
    @esphilee 4 года назад +1

    They sell darkfield condenser separately. They want you to buy that.

  • @geovannyalvarez9241
    @geovannyalvarez9241 3 года назад

    Tal vez alguien sabe como centrar el condensador en este microscopio Swift 380B

  • @vidismax
    @vidismax 11 месяцев назад

    😁😁😁👌genius, THANK YOU!

  • @antonievanleeuwenhoek5772
    @antonievanleeuwenhoek5772 4 года назад

    Sir i want to make a lab in my home sir so can can u give me some tips

  • @oliviergrimard3845
    @oliviergrimard3845 3 года назад

    I did the same thing, same microscope but everything is dark…. I opened the iris diaphragm, and light to max, plus the condenser was all the way up….

  • @THATHATSALLFOLKS
    @THATHATSALLFOLKS 4 года назад +4

    WATCH FACE GLASS ON EBAY...THEN GLUE A PENNY ON IT

  • @suky108
    @suky108 3 года назад

    Hi thanks for video find very entertaining. Can you or someone help me please.. upgraded old microscope to modern one, will a RGB led light source be a good idea, in order to for example control colour source? I believe this is different to dark field?
    I plan to get out my old wiring and substitute for usb rgb led light source that is controlled led via app/phone.
    Kind regards
    :)

  • @namnueaintarabut7348
    @namnueaintarabut7348 2 года назад

    I love your chenel

  • @buzneg
    @buzneg 3 года назад

    I tried sticking my finger over the light, on a Swift 380 and got some good dark field/contrast effects. See it here: ruclips.net/video/_MXBhgWcIjY/видео.html

  • @eliasednie
    @eliasednie Месяц назад

    Saved me $99.90
    I used a 10¢ coin
    That's worth a sub😊

  • @moneypenni1694
    @moneypenni1694 3 года назад

    New sub here, THANK YOU SO MUCH for making this vid!!!

  • @abdulkadirabdirasak3644
    @abdulkadirabdirasak3644 4 года назад

    Masha Allah

  • @Molko701
    @Molko701 4 года назад

    Thank you!

  • @RonGunsolus
    @RonGunsolus 4 года назад

    THANK YOU!