I tried your method on a mold. They were in the freezer for 4 weeks, and I incubated them again this wednesday. Checking back on saturday, I hope they will have survived
can we preserve bacterial colonies from agar medium ,without growing the bacteria in a broth ?this method do not use broth in the glycerol stock ? is it possible to store in this way?
nice video. but you probably contaminated your glycerol by using the same tip 3 times ;). I have done the same thing and it is a big hassle to work with contaminated reagents
Camroc37 I isolated them by heating a small amount of soil in a water bath up to 80°C, where most other bacteria die. But as Bacillus can make spores, they survive and you can easily streak them on LB agar plates :) Maybe I´ll make a video about that one day
All is Ok. One thing - you didn`t explain for what you using glycerol. I mean - what did the glycerol do with bacteria cell. ( Ahh, sorry: -30 degrees. ) Wikipedia says: "Like ethylene glycol and propylene glycol, glycerol is a non-ionic kosmotrope that forms strong hydrogen bonds with water molecules, competing with water-water hydrogen bonds. This disrupts the crystal lattice formation of ice unless the temperature is significantly lowered. The minimum freezing point temperature is about −36 °F (−38 °C) corresponding to 70% glycerol in water." Instead of itself glycerol melting point that is 17.8 °C.
@@kjnazari4734 you will need liquid nitrogen for kryoconservation of eucaryontic cells. By cooling the cells down at such a low temperature, the forming ice crystals are so small, that they won´t destroy the cell membrane. Your freezer will not cool the semen fast enough. But you could try to use Dimethyl Sulfoxide, which prevents the forming of ice crystals in cells :)
I tried your method on a mold. They were in the freezer for 4 weeks, and I incubated them again this wednesday. Checking back on saturday, I hope they will have survived
where did you get the centrifuge and how much did it cost?
How you made inoculation loop
can we preserve bacterial colonies from agar medium ,without growing the bacteria in a broth ?this method do not use broth in the glycerol stock ? is it possible to store in this way?
what is volum of glycerol used?
abeer gad 50%, which means if you use 200mL, you’ll need 100mL of water and the other 100mL of glycerol.😘
nice video. but you probably contaminated your glycerol by using the same tip 3 times ;). I have done the same thing and it is a big hassle to work with contaminated reagents
+Erki Sala thank you :D And yes, you´re right I did a mistake there.
Would putting my water bacteria samples in a box next to freezing cold ice keep my bacteria alive for at least a week?
No broth is needed??
How did you get your bacteria?
Camroc37 I isolated them by heating a small amount of soil in a water bath up to 80°C, where most other bacteria die. But as Bacillus can make spores, they survive and you can easily streak them on LB agar plates :)
Maybe I´ll make a video about that one day
All is Ok. One thing - you didn`t explain for what you using glycerol. I mean - what did the glycerol do with bacteria cell. ( Ahh, sorry: -30 degrees. )
Wikipedia says:
"Like ethylene glycol and propylene glycol, glycerol is a non-ionic kosmotrope that forms strong hydrogen bonds with water molecules, competing with water-water hydrogen bonds.
This disrupts the crystal lattice formation of ice unless the
temperature is significantly lowered. The minimum freezing point
temperature is about −36 °F (−38 °C) corresponding to 70% glycerol in
water." Instead of itself glycerol melting point that is 17.8 °C.
Is this done in a lab or your kitchen?
It doesn’t matter.
Nice channel, I like it!
Camroc37 Thank you!
Yours too, the graphics on your channel are on a much higher level than mine :)
thank you for the video, is it possible to use the same method for conversing semen?
Νο, you can not just freeze in eukaryontic cells and then revive them.
@@naturalscience3529 so how is semen conserved for such a long time without killing the sperms at such a freezing cold temperatures?
@@kjnazari4734 you will need liquid nitrogen for kryoconservation of eucaryontic cells. By cooling the cells down at such a low temperature, the forming ice crystals are so small, that they won´t destroy the cell membrane. Your freezer will not cool the semen fast enough. But you could try to use Dimethyl Sulfoxide, which prevents the forming of ice crystals in cells :)