I have to admit, some of these compounds are impressive tongue-twisters. I can see why they go for so many acronyms. Breathe better, everyone, and thanks again, Professor Dave!
Great explanation Prof Dave. I believe at 4:20 the shift mentioned is tilted towards more phosphatase activity rather than kinase activity. Correct me if i am wrong.
Hi, I’m the script writer. That could certainly be the case. If you have seen some compelling data that backs that up thats totally fair. The point I’m trying to get across is these enzymes both contribute to transducing the efficacy of agonists at this receptor! Relative contributions are interesting to discuss.
@@mikek4025 a phosphatase dephosphorylates, a kinase phosphorylates. hence the myosin light chain phosphatase cleaves the phosphate of the myosin light chain which causes smooth muscle relaxation
You would be correct it was probably just a missed error. Increased MLCK activity would mean more myosin light chain phosphorylation which promotes smooth muscle contraction.
I have a question: Do we actually know these reactions take place? Or is it assumed based on waste products and the fact that expected results take place? My chemistry is very rudimentary so sorry if this is a stupid question.
We know how the cells work, and we know how the drugs work and what they are (we made them, duh), so i assume these effects are known to occur. We can use organic chemistry and cell biology to figure out what drugs will do, and make drugs that do what we want. So i assume the uncertainty is very low in terms of what reactions are taking place.
There are some very intricate experiments that can demonstrate these reactions are happening. Short answer is yes we know these reactions happen this way from multiple different experiments, it’s not because of waste products, in most biological systems most proteins and indeed most chemical entities are constantly recycled
One thing I don't get is, when I have a long lasting asthma attack, the efficiency of salbutamol decreases with time. What is creating that short-term drug resistance?
Hi, short term resistance is due to a beta2 adrenergic receptor internalisation from the cell surface membrane into intracellular compartments. This means the receptor is no longer present on the membrane to be activated by salbutamol, so the signalling mechanism can’t be transduced. Excellent question
Hi, Dave! Science Journalist Dave here. I keep getting sucked into your old flat Earth videos. RUclips just keeps pushing them at me. I have one request. Replace "in actuality"with actually, please. Just a small thing that you may not do anymore anyway. Thanks for all your work.
The academic tutorials never get tons of views on the day they are released, they accumulate views over time as students need them for their coursework.
massive resepct from me. you could make another anti-vaccine, political, or flat earth video and ger 40 trillion views, but you stay true to your content instead. respect from me.
I have to admit, some of these compounds are impressive tongue-twisters. I can see why they go for so many acronyms. Breathe better, everyone, and thanks again, Professor Dave!
Asthmatics, assemble!!!
Yeah (puff) just give me a sec (huff) gotta find my salbutamol
i was just prescribed albuterol lol. cool to see how it works
You really should make a video on the Magneto-Turboencabulator this incoming April
Great explanation Prof Dave. I believe at 4:20 the shift mentioned is tilted towards more phosphatase activity rather than kinase activity. Correct me if i am wrong.
Hi, I’m the script writer. That could certainly be the case. If you have seen some compelling data that backs that up thats totally fair. The point I’m trying to get across is these enzymes both contribute to transducing the efficacy of agonists at this receptor! Relative contributions are interesting to discuss.
@@mikek4025
a phosphatase dephosphorylates, a kinase phosphorylates. hence the myosin light chain phosphatase cleaves the phosphate of the myosin light chain which causes smooth muscle relaxation
@@lukas4235 yes
You would be correct it was probably just a missed error. Increased MLCK activity would mean more myosin light chain phosphorylation which promotes smooth muscle contraction.
@@Steven_Dunbar thanks
thanks, this is an important topic
When you have a "part N" video, can you please put a link to the earlier part(s) in the description? Right now, I'd like to find part 1 of this.
Just check the pharmacology playlist linked in the description it’ll be right before this one.
I have a question: Do we actually know these reactions take place? Or is it assumed based on waste products and the fact that expected results take place? My chemistry is very rudimentary so sorry if this is a stupid question.
We know how the cells work, and we know how the drugs work and what they are (we made them, duh), so i assume these effects are known to occur. We can use organic chemistry and cell biology to figure out what drugs will do, and make drugs that do what we want. So i assume the uncertainty is very low in terms of what reactions are taking place.
There are some very intricate experiments that can demonstrate these reactions are happening. Short answer is yes we know these reactions happen this way from multiple different experiments, it’s not because of waste products, in most biological systems most proteins and indeed most chemical entities are constantly recycled
Can I know sources you used to write for this video ? because I have a report about salbutamol and I need to added some sources when I write it😢
One thing I don't get is, when I have a long lasting asthma attack, the efficiency of salbutamol decreases with time. What is creating that short-term drug resistance?
Hi, short term resistance is due to a beta2 adrenergic receptor internalisation from the cell surface membrane into intracellular compartments. This means the receptor is no longer present on the membrane to be activated by salbutamol, so the signalling mechanism can’t be transduced. Excellent question
Unfortunately, yes, that happens. It is called, tachyphylaxis.
Hi, Dave!
Science Journalist Dave here.
I keep getting sucked into your old flat Earth videos.
RUclips just keeps pushing them at me.
I have one request. Replace "in actuality"with actually, please. Just a small thing that you may not do anymore anyway. Thanks for all your work.
Awesome 😎😎😎
Can salbutamol relax the uterine smooth muscle, which contracts during menstrual periods causing pain?
What happened to your channel, you deserve more views
The academic tutorials never get tons of views on the day they are released, they accumulate views over time as students need them for their coursework.
God bless you Professor David, very interesting video 👏
I'm sad this Chanel is almost dead :(
November was the best month in the history of my channel.
@@ProfessorDaveExplains I'm not being toxic man but really do love this Chanel even my science teacher use u as guide thanks man but wow ty
Yooo I'm EARLY!
Professor Dave, how do I message you directly?
massive resepct from me. you could make another anti-vaccine, political, or flat earth video and ger 40 trillion views, but you stay true to your content instead. respect from me.
👏👏
cocaine is the best bronchodilator!
Disgusting verbalism
Um, what?