Hi Habeebah, we would not recommend using a Thermo Mixer for the bead beating step. The Thermo Mixer will just shake the beads, but it won't move them in the right orientation so that they break open the microbial cells. In this case, you most likely will end up with low yields and underrepresentation of harder-to-lyse microbes like gram-positive bacteria or fungi. Here is a list of bead beating instruments and conditions that we would recommend: files.zymoresearch.com/documents/bead_beating_short_protocol_tables.pdf The easiest to set up would be the Vortex Genie from Scientific Industries with a Horizontal Tube Adapter (see links below): www.zymoresearch.com/products/vortex-genie-2 www.zymoresearch.com/products/horizontal-microtube-holder
Hi Maxine, you can use the PowerLyzer 24 Homogenizer for the bead beating steps. I believe that instrument is very similar to the Omni Bead Ruptor Elite instrument, so you can use those conditions as we've outlined here in our Optimized Lysis Protocols sheet files.zymoresearch.com/documents/bead_beating_short_protocol_tables.pdf:
How do i store the filtered dna?
Hi Kevin, we would recommend storing the purified DNA in either a -20C or -80C freezer to ensure long term stability.
Hello Zymo, Can i use a thermo mixer in place of Bead beater?
Hi Habeebah, we would not recommend using a Thermo Mixer for the bead beating step. The Thermo Mixer will just shake the beads, but it won't move them in the right orientation so that they break open the microbial cells. In this case, you most likely will end up with low yields and underrepresentation of harder-to-lyse microbes like gram-positive bacteria or fungi.
Here is a list of bead beating instruments and conditions that we would recommend: files.zymoresearch.com/documents/bead_beating_short_protocol_tables.pdf
The easiest to set up would be the Vortex Genie from Scientific Industries with a Horizontal Tube Adapter (see links below):
www.zymoresearch.com/products/vortex-genie-2
www.zymoresearch.com/products/horizontal-microtube-holder
Hi, can I use a power lyzer instead of the bead beater? and for how long? Thanks !
Hi Maxine, you can use the PowerLyzer 24 Homogenizer for the bead beating steps. I believe that instrument is very similar to the Omni Bead Ruptor Elite instrument, so you can use those conditions as we've outlined here in our Optimized Lysis Protocols sheet
files.zymoresearch.com/documents/bead_beating_short_protocol_tables.pdf:
nice