I love this video. I hope you still continue to make this kind of content coz this really simplifies the topic despite its complexity. keep up the good content. Salamat!!!!
Why would you subtract the 0.85% NaCl solution from the absorbance values if you are using it as the blank? Could you not just zero out the spectrophotometer with the 0.85% blank? If so, would it not just be the absorbance of the sample tube divided by the absorbance of the negative control tube, multiplied by 100?
Hi. The blank being used here is plain 0.85% NaCl without blood. Meanwhile for the equation, the absorbance being deducted is from the supernatant of the 0.85% NaCl tube with blood. Please check out this video for the demonstration: ruclips.net/video/NWn3gpXnS1k/видео.html
Nicely presented topic. Well done
I love this video. I hope you still continue to make this kind of content coz this really simplifies the topic despite its complexity. keep up the good content. Salamat!!!!
HI! What are the advantages and disadvantages of Sanford and Dacie's method?
Why would you subtract the 0.85% NaCl solution from the absorbance values if you are using it as the blank? Could you not just zero out the spectrophotometer with the 0.85% blank? If so, would it not just be the absorbance of the sample tube divided by the absorbance of the negative control tube, multiplied by 100?
Hi. The blank being used here is plain 0.85% NaCl without blood. Meanwhile for the equation, the absorbance being deducted is from the supernatant of the 0.85% NaCl tube with blood. Please check out this video for the demonstration: ruclips.net/video/NWn3gpXnS1k/видео.html
@@ClinicalLabScienceVideos Ok, I'm sorry. I missed that during my initial watch. Thanks for clarifying.
Thankyou
Is it the release of hemoglobin that causes colour differences
?
Yes it is.