Agilent 6230 LC/MS: Introduction and Overview

[@srikanthk6907]

Can we perform quantitative analysis with this instrument?

[@federicocortese777]

Yes, but you must use an analytical standard to quantify one specific molecule in your sample. For each new molecule you want to quantify, you need to have the corresponding analytical standard

[@srikanthkomakula211]

@@federicocortese777 Thanks for the reply. We have been trying with a standard for a particular compound but the response for the same concentration of the standard is changing in every analysis. What could be the reason?

[@user-tp2vc1ut4s]

I would suggest trying different known concentrations and see the response. Hope that helps

[@khalidomar5153]

I think make sure the condition is the same and when preparing standard make sure it is always the same and also the standard compound should be pure and try playing with solvent assuming you are injecting direct without using.