Prothrombin Time Test.PT Test Method in HumaClot Duo Plus

Prothrombin Time Test.PT Test Method in HumaClot Duo Plus
Reference Range
For normal plasma a range of PT = 10-14 seconds can be expected.
Reagent Preparation
Reconstitute 1 vial of [RGT] with the entire content of 1 vial of [BUF] of the same lot.
Keep [RGT] warm at 37°C for at least 30 minutes for proper reconstitution. Swirl the
vial gently, horizontally more times (5-10) before use, but do not shake.
Storage and Stability
When stored at 2...8°C unopened vials are stable until the expiry date.
After reconstitution, [RGT] is stable for 12 days at 2...8°C and 24 hours at 22°C. Store
at 2...8°C when not in use. Do not freeze reconstituted [RGT]!
Specimen Collection
Use 3.2% sodium citrate as anticoagulant. The use of higher concentration of
trisodium citrate (3.8 %; 129 mmol/l) is not recommended. Obtain venous blood by
clean venipuncture. Immediately mix 9 parts of blood with 1 part of anticoagulant.3
Avoid foaming the specimen.
Specimen Processing
Centrifuge the blood specimen for 15 minutes at 1500 x g to yield platelet poor or
platelet free plasma. Remove plasma using a plastic pipette and store in a plastic
tube. Cover specimens to prevent pH changes that may affect test results3.
Specimens maintained at 22...24°C must be tested within 2 hours. Plasma held at
2...8°C may undergo cold activation resulting in significant shortening of the PT. For
longer periods of time, plasma should be frozen at -20°C for up to 2 weeks or at -70°C
for up to 6 months. Thaw samples rapidly at 37°C, swirl gently and test immediately.
Do not re-freeze.3
Safety Note
All materials contaminated with patient specimens, reagent and controls should
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