Plate Coating with Matrigel®

In this video, we will be going over the coating protocol to maintain induced pluripotent stem cells (iPSCs). Matrix coating is required to support cell attachment and expansion. At the EDDU, we use Matrigel to coat our dishes. Matrigel matrix is sensitive to temperature and will start to form a gel at 10 °C and will rapidly gel at temperatures greater than 22 °C. Matrigel is stored in a -80 °C freezer in individual aliquots for single use. DO NOT refreeze Matrigel.
List of Materials:
- Matrigel aliquot
- Cold DMEM/F12 media with Antibiotic-Antimycotic
- 10 mL plastic serological pipette
- Sterile pipette tips
- 15 mL conical centrifuge tube
- Sterile culture dish (6 or 10 cm)
For more information about the work being done at The Neuro's EDDU, please visit us at:
Website - www.mcgill.ca/...
Data portal - www.neuro-eddu...
Instagram - / neuro_eddu
LinkedIn - / the-neuro-eddu

[@Yhusi]

Thank for the usefull video. Do we need to remove the matrigel before seeding the cells?

[@user-sh2ct4sk2o]

thanks!

[@MontrealNeuro]

You are welcome

[@rakaaziz8479]

Thank you for the tutorial. Can we use any other variation of DMEM medium?

[@ratihrinendyaputri6841]

Is this 3D culture?

[@MontrealNeuro]

Hi Ratih, thanks for your message. This is the protocol to prepare matrigel-coated dishes for 2D cultures. We do have a video on how to make midbrain organoids: ruclips.net/video/RPepzaa6gic/видео.html

[@canbeone7277]

no

[@Yhusi]

Thank for the usefull video. Do we need to remove the matrigel before seeding the cells?

[@MontrealNeuro]

Yes, please remove the matrigel solution by aspiration before seeding the cells in the appropriate cell culture media.